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Mannheimia ovis sp. nov., Isolated from Dead Sheep with Hemorrhagic Pneumonia

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Abstract

Two Gram-stain-negative, facultatively anaerobic bacteria, designated ZY170218T and ZY180512, were isolated from lungs of dead sheep with hemorrhagic pneumonia in Yunnan Province, China and their taxonomic positions were studied by a polyphasic approach. The two isolates grew optimally at 37 °C, pH 9.0 and 1.0% NaCl (w/v), and showed identical 16S rRNA, recN and rpoB gene sequences. Phylogenetic analysis based on 16S rRNA gene sequence showed that the two strains fell within the cluster of species in the genus Mannheimia and formed a separated lineage with comparatively low similarity to the closest related species M. granulomatis (96.5%). Phylogenetic analysis based on rpoB gene indicated that the strains formed a monophyletic evolutionary lineage, with low sequence similarity ≤ 89.0% to the species of the genus Mannheimia. The genomic OrthoANI values between strain ZY170218T and M. granulomatis and M. haemolytica were 80.4% and 83.1%, respectively. The genomic G + C content of strain ZY170218T was 39.1 mol%. The predominant fatty acids (> 5%) of the two strains were C16:0, C14:0, C18:1ω7c, summed feature 3 (C16:1 ω7c and/ or C16:1ω6c) and summed feature 2 (C14:0 3OH/ C16:1 Iso). The major polar lipids of strain ZY170218T were phosphatidylglycerol, phosphatidylcholine, monogalactosyldiacylglycerol, bis(monoacylglycero)phosphate and diacylglycerols. The sole respiratory quinone of the two strains was CoQ-7. On the basis of phylogenetic, phenotypic and chemotaxonomic features, strain ZY170218T and ZY180512 clearly represents a novel species of the genus Mannheimia, for which the name Mannheimia ovis sp. nov. is proposed. The type strain is ZY170218T (= CGMCC 1.13620 T = KCTC 15731 T).

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Data Availability

The GenBank/EMBL/DDBJ accession number for the 16S rRNA, recN, rpoB gene and complete genome sequence of strain ZY170218T is MH254941, MN745099, MN745097 and CP046531, respectively. The GenBank/EMBL/DDBJ accession number for the 16S rRNA, recN and rpoB gene of strain ZY1180512 is MT113948, MT113950 and MT113949, respectively.

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Acknowledgements

This work was supported by grant from the Open Foundation of the State Key Laboratory of Veterinary Etiological Biology (BYSWX2018KFKT12) and the National Key Research and Development Program of China (No. 2017YFD0501800).

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Authors and Affiliations

  1. Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, 650224, P. R. China

    Fuxiang Li, Wenhua Zhao, Jianbo Zhu & Shibiao Yang

  2. Yunnan Provincial Meat Caprine Engineering Research Center, Yunnan Animal Science and Veterinary Institute, Kunming, 650224, P. R. China

    Qionghua Hong & Qingyong Shao

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  1. Fuxiang Li
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  2. Wenhua Zhao
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  3. Jianbo Zhu
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  6. Shibiao Yang
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Contributions

FL isolated the strain, performed morphological, biochemical and chemotaxonomic analysis and wrote the manuscript, WZ and QS performed the phylogenetic analysis, JZ performed the genomic analysis, SY and QH supervised the study and revised the manuscript. All authors read and approved the final version of the manuscript.

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Correspondence to Shibiao Yang.

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Li, F., Zhao, W., Zhu, J. et al. Mannheimia ovis sp. nov., Isolated from Dead Sheep with Hemorrhagic Pneumonia. Curr Microbiol 77, 3504–3511 (2020). https://doi.org/10.1007/s00284-020-02209-7

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  • DOI: https://doi.org/10.1007/s00284-020-02209-7

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