Abstract
Energy crisis and environmental sustainability have attracted global attention to microalgal biofuels. The present study investigated the impact of organic carbon sources on growth and bio-oil accumulation by an oleaginous microalga Desmodesmus subspicatus LC172266 under mixotrophic culture condition. Glucose and glycerol supported higher growth rates and lipid productivities than sucrose, fructose, mannitol and acetate. Each of the organic carbon source tested supported significantly (P < 0.05) higher growth rates and lipid productivities than the photoautotrophic culture (without organic carbon source). The lipid productivity obtained with a mixture of optima concentrations of glucose and glycerol (5.0 gL−1 glycerol + 10.0 gL−1glucose) (0.14875 ± 0.002 g/L/day) was about 25% and 66% higher than the values obtained with only 10.0 gL−1glucose and 5.0 gL−1glycerol, respectively. When a batch culture with 5gL−1glycerol was fed with 0.5 gL−1glucose daily the cell growth and lipid productivity were lower than the values obtained in a batch culture with a mixture of glucose and glycerol. The lipid productivity obtained in a 4-L photobioreactor was 94% (0.217 gL−1 day−1), higher than the value obtained in a flask culture with 10.0 g/Lglucose (0.112 gL−1 day−1) and 46% higher than the value obtained in a flask culture with 5.0 gL−1glycerol (0.086 gL−1 day−1).
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The data sets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Financial support from the Tertiary Education Trust fund of the Federal Republic of Nigeria is acknowledged.
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All authors contributed to the study conception and design. Material preparation and data collection were done by CNE and JCO, and analysis were performed by CNE, JCO and IOO. HA provided technical support and supervision. The first draft of the manuscript was written by CNE and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Communicated by Erko Stackebrandt.
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Eze, C.N., Ogbonna, I.O., Aoyagi, H. et al. Effects of organic carbon sources on growth and oil accumulation by Desmodesmus subspicatus LC172266 under mixotrophic condition. Arch Microbiol 204, 553 (2022). https://doi.org/10.1007/s00203-022-03165-5
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DOI: https://doi.org/10.1007/s00203-022-03165-5