Abstract
Key message
Two major-effect QTL GlcA07.1 and GlcA09.1 for green leaf color were fine mapped into 170.25 kb and 191.41 kb intervals on chromosomes A07 and A09, respectively, and were validated by transcriptome analysis.
Abstract
Non-heading Chinese cabbage (NHCC) is a leafy vegetable with a wide range of green colors. Understanding the genetic mechanism behind broad spectrum of green may facilitate the breeding of high-quality NHCC. Here, we used F2 and F7:8 recombination inbred line (RIL) population from a cross between Wutacai (dark-green) and Erqing (lime-green) to undertake the genetic analysis and quantitative trait locus (QTL) mapping in NHCC. The genetic investigation of the F2 population revealed that the variation of green leaf color was controlled by two recessive genes. Six pigments associated with green leaf color, including total chlorophyll, chlorophyll a, chlorophyll b, total carotenoids, lutein, and carotene were quantified and applied for QTL mapping in the RIL population. A total of 7 QTL were detected across the whole genome. Among them, two major-effect QTL were mapped on chromosomes A07 (GlcA07.1) and A09 (GlcA09.1) corresponding to two QTL identified in the F2 population. The QTL GlcA07.1 and GlcA09.1 were further fine mapped into 170.25 kb and 191.41 kb genomic regions, respectively. By comparing gene expression level and gene annotation, BraC07g023810 and BraC07g023970 were proposed as the best candidates for GlcA07.1, while BraC09g052220 and BraC09g052270 were suggested for GlcA09.1. Two InDel molecular markers (GlcA07.1-BcGUN4 and GlcA09.1-BcSG1) associated with BraC07gA023810 and BraC09g052220 were developed and could effectively identify leaf color in natural NHCC accessions, suggesting their potential for marker-assisted leaf color selection in NHCC breeding.
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Data availability
The raw data has been deposited in Non-heading Chinese Cabbage and Watercress Database (NHCCP10001) (http://tbir.njau.edu.cn/NhCCDbHubs/DownloadDetail_detail.action?download_fileType=submitdata).
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Acknowledgements
The authors thank Xin Yu and Liping Tang provided valuable advice and revised the manuscript. This work was supported by the Bioinformatics Center of Nanjing Agricultural University.
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This work was supported by Jiangsu Seed Industry Revitalization Project (JBGS(2021)015), National Vegetable Industry Technology System (CARS-23-A-16) and the National Natural Science Foundation of China (32172562 and 31872106), the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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AMB performed majority of the reported work. Ying.L designed and supervised this experiment. HBW, FXZ, TZZ, and LG participated in filed trial, phenotype measurement, and InDel genotyping. Yan.L participated in phenotypic data collection. YHW participated in QTL mapping and data analysis. SHAS, TKL, and XLH participated in manuscript revision. AMB, YHW, and Ying.L wrote the manuscript with input from other coauthors. All authors reviewed and approved this submission.
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Bai, A., Zhao, T., Li, Y. et al. QTL mapping and candidate gene analysis reveal two major loci regulating green leaf color in non-heading Chinese cabbage. Theor Appl Genet 137, 105 (2024). https://doi.org/10.1007/s00122-024-04608-x
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DOI: https://doi.org/10.1007/s00122-024-04608-x