Abstract
The orange inner leaf of the Chinese cabbage is controlled by a single recessive gene (or), which causes abnormal accumulation of carotene. In the present study, an F2 population consisting of 600 individuals was used for mapping or and developing new markers closely linked to this gene. Bulked segregant analysis was performed by screening 435 simple sequence repeat (SSR) markers well-distributed on 10 linkage groups and 16 SSR primers derived from nine bacterial artificial chromosome (BAC) clones. On the basis of linkage analysis, the or gene was mapped in a region covering a total interval of 4.6 centimorgans (cM) between two SSR markers derived from BAC clones AC172873 and AC189246 at the end of linkage group 9, which matches with chromosome 1 of A genome in Chinese cabbage. A genetic map of the or locus was constructed by using five SSR markers and two morphological markers. Three SSR markers were tightly linked to or and two of them, sau (C) 586 and syau19, were located on the same side at distances of 1.6 and 1.3 cM, respectively. The other marker, syau15, was located on the other side at a distance of 3.3 cM. The two morphological markers, orange flower and orange cotyledon (before cotyledon turns green during the germination period), were obtained by visual determination and screening of the differences in the morphological traits between parents and the two segregated F2 populations; the two markers were designated as or-f (orange flower) and or-c (orange cotyledon). It was suggested that these two markers co-segregate with orange inner leaf trait or that the three characters, namely orange inner leaf, orange flower, and orange cotyledon, are determined by the same gene. These markers could be very helpful for marker-assisted selection in Chinese cabbage hybrid breeding programs.
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References
Chen PF (2008) The study of identify and accumulation mechanisms and regulation control of carotenoid on Chinese cabbage [D]: College of Horticulture and Protection. Yangzhou University, China
Choi SR, Teakle GR, Plaha P, Kim JH, Allender CJ, Beynon E, Piao ZY, Soengas P, Han TH, King GJ, Barker GC, Hand P, Lydiate DJ, Batley J, Edwards D, Koo DH, Bang JW, Park BS, Lim YP (2007) The reference genetic linkage map for the multinational Brassica rapa genome sequencing project. Theor Appl Genet 115:777–792
Crisp P, Walkey DGA, Bellman E, Roberts E (1975) A mutation affecting curd colour in cauliflower (Brassica oleracea L. var. botrytis DC). Euphytica 24:173–176
Dickson MH, Lee CY, Blamble AE (1988) Orange-curd high carotene cauliflower inbreds, NY 156, NY 163, and NY 165. Hort Sci 23:778–779
Dijkhuizen A, Meglic V, Staub JE, Havey MJ (1994) Linkages among RFLP, RAPD, isozyme, disease-resistance, and morphological markers in narrow and wide crosses of cucumber. Theor Appl Genet 89:42–48
Feng H, Wei P, Piao ZY, Liu ZY, Li CY, Wang YG, Ji RQ, Ji SJ, Zou T, Choi SR, Lim YP (2009) SSR and SCAR mapping of a multiple-allele male-sterile gene in Chinese cabbage (Brassica rapa L.). Theor Appl Genet 119:333–339
Frank HA, Cogdell RJ (1996) Carotenoids in photosynthesis. Photochem Photobiol 63:257–264
Fraser PF, Bramley PM (2004) The biosynthesis and nutritional uses of carotenoids. Prog Lipid Res 43:228–265
Isaacson T, Ronen G, Zamir D, Hirschberg J (2002) Cloning of tangerine from tomato reveals a carotenoid isomerase essential for the production of β-carotene and xanthophylls in plants. Plant Cell 14:333–342
Kaga A, Ohnishi M, Ishii T, Kamijima O (1996) A genetic linkage map of azuki bean constructed with molecular and morphological markers using an interspecific population (Vigna angularis x V. nakashimae). Theor Appl Genet 93:658–663
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Li L, Garvin DF (2003) Molecular mapping of Or, a gene inducing β-carotene accumulation in cauliflower (Brassica oleracea var. botrytis). Genome 47:588–594
Li L, Paolillo DJ, Parthasarathy MV, DiMuzio EM, Garvin DF (2001) A novel gene mutation that confers abnormal patterns of β-carotene accumulation in cauliflower (Brassica oleracea var. botrytis). Plant J 26:59–67
Li L, Lu S, Halloran D, Garvin DF (2003) High-resolution genetic and physical mapping of the cauliflower high-β-carotene gene Or (Orange). Mol Genet Genomics 270:132–138
Liu XC, Zhang FL, Zhang DS, Yang JP, Wang M, Xu JB (2003) Identification of a RAPD marker linked to orange-red internal head gene in Chinese cabbage. Acta Agric Boreali-Sinica 18:51–54
Lu S, Joyce VE, Zhou XJ, Alex BL, Diana MO, Kelly MC, Brian JC, Dominick JP, David FG, Julia V, Leon VK, Hendrik K, Elizabeth DE, Cao J, Li L (2006) The cauliflower Or gene encodes a DnaJ cysteine-rich domain-containing protein that mediates high levels of β-carotene accumulation. Plant Cell 18:3594–3605
Matsumoto E, Yasui C, Met Ohi, Tsukada M (1998) Linkage analysis of RFLP markers for clubroot resistance and pigmentation in Chinese cabbage (Brassica rapa ssp. pekinensis). Euphytica 104:79–86
Michelmore RW, Paran I, Kesseli RV (1991) Identification of marker linked to disease-resistance gene by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Molnar J, Gyemant N, Mucsi I, Molnar A, Szabo M, Kortvelyesi T, Varge A, Molnar P, Toth G (2004) Modulation of multidrug resistance and apoptosis of cancer cells by selected carotenoids. In Vivo 18:237–244
Mortensen A, Skibsted LH (1997) Importance of carotenoid structure in radical-scavenging reactions. J Agric Food Chem 45:2970–2977
Mun JH, Kwon SJ, Yang TJ, Kim HS, Choi BS, Baek SH, Kim JS, Jin M, Kim JA, Lim MH, Lee SI, Kim HI, Kim HT, Lim YP, Park BS (2008) The first generation of a BAC-based physical map of Brassica rapa. BMC Genomics 9:280
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4326
Olson JA (1989) Provitamin A function of carotenoids: the conversion of β-carotene into vitamin A. J Nutr 119:105
Piao ZY, Deng YQ, Choi SR, Park YJ, Lim YP (2004) SCAR and CAPS mapping of CRb, a gene conferring resistance to Plasmodiophora brassica in Chinese cabbage (Brassica rapa ssp. pekinensis). Theor Appl Genet 108:1458–1465
Pogson BJ, Niyogi KK, Bjorkman O, Dellapenna D (1998) Altered xanthophyll compositions adversely affect chlorophyll accumulation and nonphotochemical quenching in Arabidopsis mutants. Proc Natl Acad Sci USA 95:13324–13329
Rieseberg LH, Ellstrand NC (1993) What can molecular and morphological markers tell us about plant hybridization. Crit Rev Plant Sci 12:213–241
Ronen G, Carmel-Goren L, Zamir D, Hirschberg J (2000) An alternative pathway to β-carotene formation in plant chromoplasts discovered by map-based cloning of Beta and old-gold color mutations in tomato. Proc Natl Acad Sci USA 97:11102–11107
Saito M, Kubo N, Matsumoto S, Suwabe K, Tsukada M, Hirai M (2006) Fine mapping of the clubroot resistance gene, Crr3, in Brassica rapa. Theor Appl Genet 114:81–91
Staub JE, Serquen FC, Gupta M (1996) Genetic markers, map construction, and their application in plant breeding. Hort Sci 31:729–741
Tanksley SD, Ganal MW, Martin GB (1995) Chromosome landing: a paradigm for map-based gene cloning in plants with large genomes. Trends Genet 11:63–68
Trick M, Bancroft I, Lim YP (2007) The Brassica rapa genome sequencing initiative. Genes Genomes Genomics 1:35–39
Van Ooijen JW, Voorrips RE (2001) JoinMap® 3.0, Software for the calculation of genetic linkage maps. Plant Research International, Wageningen, the Netherlands
Von Lintig J, Welsch R, Bonk M, Giuliano G, Batschauer A, Kleinig H (1997) Light-dependent regulation of carotenoid biosynthesis occurs at the level of phytoene synthase expression and is mediated by phytochrome in Sinapis alba and Arabidopsis thaliana seedlings. Plant J 12:625–634
Voorrips RE (2002) MapChart, software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78
Wang GC, Zhang FL, Yu YJ, Zhang DS, Zhao XY (2007) Identification of SCAR markers linked to orange head leaf gene in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Acta Hort Sin 34:217–220
Wei P, Feng H, Piao ZY, Li CY, Liu ZY, Wang YG, Ji RQ, Zou T, Ji SJ (2009) Identification of AFLP markers linked to Ms, a genic multiple allele inherited male-sterile gene in Chinese cabbage. Breed Science 59:333–339
Yan Z, Denneboom C, Hattendorf A, Dolstra O, Debener T, Stam P, Visser PB (2005) Construction of an integrated map of rose with AFLP, SSR, PK, RGA, RFLP, SCAR and morphological markers. Theor Appl Genet 110:766–777
Yie HP, Zhu ZL, Sun MM (2005) Synergistic formulation for preventing and/or treating diabetes. US Patent App 2008/0268066
Yu SC, Zhang FL, Yu RB, Zou YM, Qi JN, Zhao XY, Yu YJ, Zhang DS, Li L (2009) Genetic mapping and localization of a major QTL for seedling resistance to downy mildew in Chinese cabbage (Brassica rapa ssp. pekinensis). Mol Breed 23:573–590
Zhang SB, Zhu Z, Zhao L, Zhang YD, Chen T, Lin J, Wang CL (2007) Identification of SSR markers closely linked to eui gene in rice. Hereditas (Beijing) 29:365–370
Zhang FL, Wang GC, Wang M, Liu XC, Zhao XY, Yu YJ, Zhang DS, Yu SC (2008) Identification of SCAR markers linked to or, a gene inducing beta-carotene accumulation in Chinese cabbage. Euphytica 164:463–471
Zhao MF, Cai CM, Che RH, Xu XZ, Zheng JH, Fang SR, Wu CZ, Shi BH (2008) Molecular mapping of photoperiod-therom sensitive male sterile gene in rice 45S. Mol Plant Breed l6:1045–1049
Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20:176–183
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This research was supported by grants from the National High Technology Research and Development Program of China (863 Program) (No. 2006AA10Z170), Specialized Research Fund for the Doctoral Program of Higher Education (20092103110001).
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Feng, H., Li, Y., Liu, Z. et al. Mapping of or, a gene conferring orange color on the inner leaf of the Chinese cabbage (Brassica rapa L. ssp. pekinensis). Mol Breeding 29, 235–244 (2012). https://doi.org/10.1007/s11032-010-9542-x
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DOI: https://doi.org/10.1007/s11032-010-9542-x