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Molecular characterization and validation of adult-plant stripe rust resistance gene Yr86 in Chinese wheat cultivar Zhongmai 895

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Abstract

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Adult-plant stripe rust resistance gene Yr86 in Chinese wheat cultivar Zhongmai 895 was mapped to the physical interval 710.2–713.2 Mb on the long arm of chromosome 2A.

Abstract

Adult-plant resistance to stripe rust is generally more durable than all-stage resistance. Chinese wheat cultivar Zhongmai 895 showed stable stripe rust resistance at the adult-plant stage. To map the genetic loci underlying its resistance, 171 doubled haploid (DH) lines from a Yangmai 16/Zhongmai 895 cross were genotyped with the wheat 660 K SNP chip. Disease severities of the DH population and parents were assessed in four environments. A major QTL designated QYryz.caas-2AL was mapped to interval 703.7–715.3 Mb on the long arm of chromosome 2A using both chip-based and KASP (kompetitive allele-specific PCR) marker-based methods, explaining 31.5 to 54.1% of the phenotypic variances. The QTL was further validated in an F2 population of cross Emai 580/Zhongmai 895 with 459 plants and a panel of 240 wheat cultivars using KASP markers. Three reliable KASP markers predicted a low frequency (7.2–10.5%) of QYryz.caas-2AL in the test panel and remapped the gene to the physical interval 710.2–713.2 Mb. Based on different physical positions or genetic effects from known genes or QTL on chromosome arm 2AL, the gene was predicted to be a new one for adult-plant stripe rust resistance and was named Yr86. Twenty KASP markers linked to Yr86 were developed in this study based on wheat 660 K SNP array and genome re-sequencing. Three of them are significantly associated with stripe rust resistance in natural population. These markers should be useful for marker-assisted selection and also provide a starting point for fine mapping and map-based cloning of the new resistance gene.

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References

  • Bansal UK, Hayden MJ, Keller B, Wellings CR, Park RF, Bariana HS (2009) Relationship between wheat rust resistance genes Yr1 and Sr48 and a microsatellite marker. Plant Pathol 58:1039–1043

    Article  CAS  Google Scholar 

  • Beddow JM, Pardey PG, Chai Y, Hurley TM, Kriticos DJ, Braun HJ, Park RF, Cuddy WS, Yonow T (2015) Research investment implications of shifts in the global geography of wheat stripe rust. Nat Plants 1:15132

    Article  PubMed  Google Scholar 

  • Chen X (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp. tritici] on wheat. Can J Plant Pathol 27:314–337

    Article  Google Scholar 

  • Chen X (2020) Pathogens which threaten food security: Puccinia striiformis, the wheat stripe rust pathogen. Food Secur 12:239–251

    Article  Google Scholar 

  • Chen X, Kang Z (2017) Stripe Rust. Springer, Dordrecht, p 444

    Book  Google Scholar 

  • Chen C, He Z, Lu J, Li J, Ren Y, Ma C, Xia X (2016) Molecular mapping of stripe rust resistance gene YrJ22 in Chinese wheat cultivar Jimai 22. Mol Breeding 36:118

    Article  Google Scholar 

  • Eriksen L, Afshari F, Christiansen MJ, McIntosh RA, Jahoor A, Wellings CR (2004) Yr32 for resistance to stripe (yellow) rust present in the wheat cultivar Carstens V. Theor Appl Genet 108:567–575

    Article  CAS  PubMed  Google Scholar 

  • Fu D, Uauy C, Distelfeld A, Blechl A, Epstein L, Chen X, Sela H, Fahima T, Dubcovsky J (2009) A kinase-START gene confers temperature-dependent resistance to wheat stripe rust. Science 323:1357–1360

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Godoy JG, Rynearson S, Chen X, Pumphrey M (2018) Genome-wide association mapping of loci for resistance to stripe rust in North American elite spring wheat germplasm. Phytopathology 108:234–245

    Article  CAS  PubMed  Google Scholar 

  • Jia M, Yang L, Zhang W, Rosewarne G, Li J, Yang E, Chen L, Wang W, Liu Y, Tong H, He W, Zhang Y, Zhu Z, Gao C (2020) Genome-wide association analysis of stripe rust resistance in modern Chinese wheat. BMC Plant Biol 20:491

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jighly A, Oyiga BC, Makdis F, Nazari K, Youssef O, Tadesse W, Abdalla O, Ogbonnaya FC (2015) Genome-wide DArT and SNP scan for QTL associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in elite ICARDA wheat (Triticum aestivum L.) germplasm. Theor Appl Genet 128:1277–1295

    Article  CAS  PubMed  Google Scholar 

  • Klymiuk V, Yaniv E, Huang L, Raats D, Fatiukha A, Chen S, Feng L, Frenkel Z, Krugman T, Lidzbarsky G, Chang W, Jääskeläinen MJ, Schudoma C, Paulin L, Laine P, Bariana H, Sela H, Saleem K, Sørensen CK, Hovmøller MS, Distelfeld A, Chalhoub B, Dubcovsky J, Korol AB, Schulman AH, Fahima T (2018) Cloning of the wheat Yr15 resistance gene sheds light on the plant tandem kinase-pseudokinase family. Nat Commun 9:3735

    Article  PubMed  PubMed Central  Google Scholar 

  • Klymiuk V, Chawla HS, Wiebe K, Ens J, Fatiukha A, Govta L, Fahima T, Pozniak CJ (2022) Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing. Commun Biol 5:826

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363

    Article  CAS  PubMed  Google Scholar 

  • Li Z, Zeng S (2002) Wheat rust in China. Chinese Agricultural Press, Beijing

    Google Scholar 

  • Liu J, He Z, Wu L, Bai B, Wen W, Xie C, Xia X (2015) Genome-wide linkage mapping of QTL for adult-plant resistance to stripe rust in a Chinese wheat population Linmai 2 × Zhong 892. PLoS ONE 10:e145462

    Article  Google Scholar 

  • Liu B, Liu T, Zhang Z, Jia Q, Wang B, Gao L, Peng Y, Jin S, Chen W (2017) Discovery and pathogenicity of CYR34, a new race of Puccinia striiformis f. sp. tritici in China. Acta Phytopathol Sin 5:681–687 (In Chinese with English abstract)

    Google Scholar 

  • Marchal C, Zhang J, Zhang P, Fenwick P, Steuernagel B, Adamski NM, Boyd L, McIntosh R, Wulff BBH, Berry S, Lagudah E, Uauy C (2018) Bed-domain-containing immune receptors confer diverse resistance spectra to yellow rust. Nat Plants 4:662–668

    Article  CAS  PubMed  Google Scholar 

  • McDonald BA, Linde C (2002) Pathogen population genetics, evolutionary potential, and durable resistance. Annu Rev Phytopathol 40:349–379

    Article  CAS  PubMed  Google Scholar 

  • McIntosh R, Mu J, Han D, Kang Z (2018) Wheat stripe rust resistance gene Yr24/Yr26: A retrospective review. Crop J 6:321–329

    Article  Google Scholar 

  • McIntosh RA, Dubcovsky J, Rogers WJ, Xia XC, Raupp WJ (2020) Catalogue of gene symbols for wheat: 2020 supplement. Annu Wheat Newsl 66:109–128

    Google Scholar 

  • Meng L, Li H, Zhang L, Wang J (2015) QTL IciMapping: Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. Crop J 3:269–283

    Article  Google Scholar 

  • Milus EA, Lee KD, Brown-Guedira G (2015) Characterization of stripe rust resistance in wheat lines with resistance gene Yr17 and implications for evaluating resistance and virulence. Phytopathol 105:1123–1130

    Article  Google Scholar 

  • Moore JW, Herrera-Foessel S, Lan C, Schnippenkoetter W, Ayliffe M, Huerta-Espino J, Lillemo M, Viccars L, Milne R, Periyannan S, Kong X, Spielmeyer W, Talbot M, Bariana H, Patrick JW, Dodds P, Singh R, Lagudah E (2015) A recently evolved hexose transporter variant confers resistance to multiple pathogens in wheat. Nat Genet 47:1494–1498

    Article  CAS  PubMed  Google Scholar 

  • Naruoka Y, Garland-Campbell KA, Carter AH (2015) Genome-wide association mapping for stripe rust (Puccinia striiformis f. sp. tritici) in US Pacific Northwest winter wheat (Triticum aestivum L.). Theor Appl Genet 128:1083–1101

    Article  CAS  PubMed  Google Scholar 

  • Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity of leaves and stems of cereals. Can J Res 26:496–500

    Article  Google Scholar 

  • Rosewarne GM, Herrera-Foessel SA, Singh RP, Huerta-Espino J, Lan C, He Z (2013) Quantitative trait loci of stripe rust resistance in wheat. Theor Appl Genet 126:2427–2449

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Singh RP, Huerta-Espino J, William HM (2005) Genetics and breeding for durable resistance to leaf and stripe rusts in wheat. Turk J Agric for 29:121–127

    CAS  Google Scholar 

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JOINMAP. Plant J 3:739–744

    Article  CAS  Google Scholar 

  • Wang Z, Ren J, Du Z, Che M, Zhang Y, Quan W, Jiang X, Ma Y, Zhao Y, Zhang Z (2019) Identification of a major QTL on chromosome arm 2AL for reducing yellow rust severity from a Chinese wheat landrace with evidence for durable resistance. Theor Appl Genet 132:457–471

    Article  CAS  PubMed  Google Scholar 

  • Wang H, Zou S, Li Y, Lin F, Tang D (2020) An ankyrin-repeat and WRKY-domain-containing immune receptor confers stripe rust resistance in wheat. Nat Commun 11:1353

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wellings CR (2011) Global status of stripe rust: a review of historical and current threats. Euphytica 179:129–141

    Article  Google Scholar 

  • Wu J, Xu D, Fu L, Wu L, Hao W, Li J, Dong Y, Wang F, Wu Y, He Z, Si H, Ma C, Xia X (2022) Fine mapping of a stripe rust resistance gene YrZM175 in bread wheat. Theor Appl Genet 135:3485–3496

    Article  CAS  PubMed  Google Scholar 

  • Xu X, Zhu Z, Jia A, Wang F, Wang J, Zhang Y, Fu C, Fu L, Bai G, Xia X, Hao Y, He Z (2020) Mapping of QTL for partial resistance to powdery mildew in two Chinese common wheat cultivars. Euphytica 216:3

    Article  CAS  Google Scholar 

  • Zeng Q, Wu J, Liu S, Chen X, Yuan F, Su P, Wang Q, Huang S, Mu J, Han D, Kang Z (2019) Genome-wide mapping for stripe rust resistance loci in common wheat cultivar Qinnong 142. Plant Dis 103:439–447

    Article  CAS  PubMed  Google Scholar 

  • Zeng Q, Zhao J, Wu J, Zhan G, Han D, Kang Z (2022) Wheat stripe rust and integration of sustainable control strategies in China. Front Agr Sci Eng 9:37–51

    Article  Google Scholar 

  • Zhang C, Huang L, Zhang H, Hao Q, Lyu B, Wang M, Epstein L, Liu M, Kou C, Qi J, Chen F, Li M, Gao G, Ni F, Zhang L, Hao M, Wang J, Chen X, Luo M-C, Zheng Y, Wu J, Liu D, Fu D (2019) An ancestral NB-LRR with duplicated 3’ UTRs confers stripe rust resistance in wheat and barley. Nat Commun 10:4023

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhang Y, Yan J, Xiao Y, Hao Y, Zhang Y, Xu K, Cao S, Tian Y, Li S, Yan J, Zhang Z, Chen X, Wang D, Xia X, He Z (2021) Characterization of wheat cultivar Zhongmai 895 with high yield potential, broad adaptability, and good quality. Sci Agri Sin 54:3158–3167 (In Chinese with English abstract)

    Google Scholar 

  • Zhou X, Wang W, Wang L, Hou D, Jing J, Wang Y, Xu Z, Yao Q, Yin J, Ma D (2011) Genetics and molecular mapping of genes for high temperature resistance to stripe rust in wheat cultivar Xiaoyan 54. Theor Appl Genet 123:431–438

    Article  CAS  PubMed  Google Scholar 

  • Zhu Z, Xu X, Fu L, Wang F, Dong Y, Fang Z, Wang W, Chen Y, Gao C, He Z, Xia X, Hao Y (2021) Molecular mapping of quantitative trait loci for Fusarium head blight resistance in a doubled haploid population of Chinese bread wheat. Plant Dis 105:1339–1345

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, for critical review of the draft manuscript, and thank Dr. Caixia Lan at Huazhong Agricultural University for providing Pst inoculum for the Ezhou site in 2021.

Funding

This study was financially supported by the National Key Research and Development Program of China (2022YFD1200900 and 2020YFE0202300), the National Natural Science Foundation of China (31961143007), the Agricultural Science and Technology Innovation Program (CAAS-ZDRW202109) and Fundamental Research Funds for Central Non-Profit of Institute of Crop Sciences, CAAS.

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Author notes

  1. Zhanwang Zhu and Qiang Cao contributed equally to this work.

Authors and Affiliations

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081, China

    Zhanwang Zhu, Qiang Cao, Jingyang Tong, Xiaowan Xu, Yong Zhang, Fengju Wang, Yachao Dong, Zhonghu He, Xianchun Xia & Yuanfeng Hao

  2. Food Crops Institute, Hubei Academy of Agricultural Sciences/Wheat Disease Biology Research Station for Central China, Wuhan, 430064, Hubei, China

    Zhanwang Zhu & Chunbao Gao

  3. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Qiang Cao, Dejun Han & Jianhui Wu

  4. Crop Research Institute, Sichuan Academy of Agricultural Sciences, Environment Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory, Chengdu, 610066, Sichuan, China

    Ling Wu

  5. Institute of Cotton Research, CAAS, Anyang, 455000, Henan, China

    Jun Yan & Kaijie Xu

  6. CIMMYT-China Office, C/O CAAS, Beijing, 100081, China

    Zhonghu He

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Contributions

ZZ and QC performed the experiment. ZZ wrote the paper. JW, DH, LW, and CG participated in field trials. JW, JT contributed to seedling test of stripe rust resistance. XX and YD assisted in marker development. JY, YZ, KX, and ZH participated in data analysis. FW assisted in population development. XX and YH designed the experiment and assisted in writing the paper. All authors have reviewed the manuscript.

Corresponding authors

Correspondence to Xianchun Xia or Yuanfeng Hao.

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The authors have no relevant financial or non-financial interests to disclose.

Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Communicated by Lee Hickey.

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Zhu, Z., Cao, Q., Han, D. et al. Molecular characterization and validation of adult-plant stripe rust resistance gene Yr86 in Chinese wheat cultivar Zhongmai 895. Theor Appl Genet 136, 142 (2023). https://doi.org/10.1007/s00122-023-04374-2

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