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R2R3 MYB transcription factor SbMYBHv33 negatively regulates sorghum biomass accumulation and salt tolerance

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Abstract

Key Message

SbMYBHv33 negatively regulated biomass accumulation and salt tolerance in sorghum and Arabidopsis by regulating reactive oxygen species accumulation and ion levels.

Abstract

Salt stress is one of the main types of environmental stress leading to a reduction in crop yield worldwide. Plants have also evolved a variety of corresponding regulatory pathways to resist environmental stress damage. This study aimed to identify a SbMYBHv33 transcription factor that downregulates in salt, drought, and abscisic acid (ABA) in the salt-tolerant inbred line sorghum M-81E. The findings revealed that overexpression of SbMYBHv33 in sorghum significantly reduced sorghum biomass accumulation at the seedling stage and also salinity tolerance. Meanwhile, a heterologous transformation of Arabidopsis with SbMYBHv33 produced a similar phenotype. The loss of function of the Arabidopsis homolog of SbMYBHv33 resulted in longer roots and increased salt tolerance. Under normal conditions, SbMYBHV33 overexpression promoted the expression of ABA pathway genes in sorghum and inhibited growth. Under salt stress conditions, the gene expression of SbMYBHV33 decreased in the overexpressed lines, and the promotion of these genes in the ABA pathway was attenuated. This might be an important reason for the difference in growth and stress resistance between SbMYBHv33-overexpressed sorghum and ectopic expression Arabidopsis. Hence, SbMYBHv33 is an important component of sorghum growth and development and the regulation of salt stress response, and it could negatively regulate salt tolerance and biomass accumulation in sorghum.

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Data availability

The data presented in this study can be freely accessed by any researcher wishing to use them for non-commercial purposes. Na Sui (suina@sdnu.edu.cn) can be reached for any data-related requests in accordance with the policy described in the Instructions for Authors (www.springer.com).

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Funding

We are grateful for the financial support from the National Natural Science Research Foundation of China (31871538 and U1906204), the National Key R&D Program of China (2018YFD1000700 and 2018YFD1000704), the Development Plan for Youth Innovation Team of Shandong Province (2019KJE012), the Shandong Province Key Research and Development Program (2019GSF107079), and the Science and Technology Demonstration Project of “Bohai Granary” of Shandong Province (2019BHLC002).

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  1. Hongxiang Zheng, Yinping Gao, Yi Sui contributed equally to this work.

Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, 250014, China

    Hongxiang Zheng, Yinping Gao, Yingying Dang, Fenghui Wu, Xuemei Wang, Fangning Zhang, Xihua Du & Na Sui

  2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Yi Sui

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  1. Hongxiang Zheng
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Contributions

HZ, XD, and NS initiated the manuscript preparation. YS performed the transformation of sorghum. HZ, YG, YD, FW, XW, and FZ performed the experiments. NS conceptualized the idea and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Xihua Du or Na Sui.

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Zheng, H., Gao, Y., Sui, Y. et al. R2R3 MYB transcription factor SbMYBHv33 negatively regulates sorghum biomass accumulation and salt tolerance. Theor Appl Genet 136, 5 (2023). https://doi.org/10.1007/s00122-023-04292-3

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