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Characterization of a novel cotton MYB gene, GhMYB108-like responsive to abiotic stresses

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Abstract

Transcriptional factors are the major regulators of plant signaling pathways in response to environmental stresses i.e., drought, salinity and cold. Hereby, the GhMYB108-like was characterized to determine whether it regulate these stresses. The GhMYB108-like cDNA consisted of 1107 base pairs (bp) with 807 open reading frame encoded a protein of 268 amino acids. Its isoelectric point and molecular weight are 5.51 and 30.3 kDa respectively. Phylogenetic analysis and online databases revealed that GhMYB108-like proteins are closely related with the Arabidopsis thaliana MYB2. Important cis-elements were detected in the promotor region of GhMYB108-like responding to stresses and phytohormones. The 3D structure of GhMYB108-like protein has been predicted. In addition, various physico-chemical properties of GhMYB108-like have been determined. Subcellular localization confirmed that GhMYB108-like are nuclear localized protein. Quantitative expression analysis showed that polyethylene glycol and salt treatments significantly induced the expression of GhMYB108-like. Overall, our findings suggest that GhMYB108-like is an important gene that would plays important regulatory role in response to drought and salt stresses.

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Acknowledgements

I am grateful to Chinese Scholarship Council who financially supported me during my PhD study. This study was funded by the National Key Project of Research and the Development Plan (2016YFD0101006). There is no conflict of interest between the authors.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Abid Ullah, Muhammad Tahir Ul Qamar, Aamir Hamid Khan & Xiyan Yang

  2. Department of Botany, University of Malakand, Chakdara Dir Lower, 18800, Khyber Pakhtunkhwa, Pakistan

    Abid Ullah, Mohammad Nisar, Ali Hazrat & Gul Rahim

  3. Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Muhammad Tahir Ul Qamar

  4. Key Laboratory of Urban Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Kashif Hayat

  5. Department of Zoology, University of Malakand, Chakdara Dir Lower, 18800, Khyber Pakhtunkhwa, Pakistan

    Saeed Ahmed

  6. Department of Biotechnology, University of Malakand, Chakdara Dir Lower, 18800, Khyber Pakhtunkhwa, Pakistan

    Waqar Ali

  7. Key Laboratory of Plant Genetic and Breeding, College of Agriculture, Guanxi University, Nanning, 530005, Guanxi, China

    Aziz khan

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  1. Abid Ullah
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Correspondence to Abid Ullah or Xiyan Yang.

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Ullah, A., Ul Qamar, M.T., Nisar, M. et al. Characterization of a novel cotton MYB gene, GhMYB108-like responsive to abiotic stresses. Mol Biol Rep 47, 1573–1581 (2020). https://doi.org/10.1007/s11033-020-05244-6

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  • DOI: https://doi.org/10.1007/s11033-020-05244-6

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