Abstract
Main conclusion
Mutation of OsSHR2 adversely impacted root and shoot growth and impaired plant response to N conditions, further reducing the yield per plant.
Abstract
Nitrogen (N) is a crucial factor that regulates the plant architecture. There is still a lack of research on it. In our study, it was observed that the knockout of the SHORTROOT 2 (OsSHR2) which was induced by N deficiency, can significantly affect the regulation of plant architecture response to N in rice. Under N deficiency, the mutation of OsSHR2 significantly reduced root growth, and impaired the sensitivity of the root meristem length to N deficiency. The mutants were found to have approximately a 15% reduction in plant height compared to wild type. But mutants showed a significant increase in tillering at post-heading stage, approximately 26% more than the wild type, particularly in high N conditions. In addition, due to reduced seed setting rate and 1000-grain weight, mutant yield was significantly decreased by approximately 33% under low N fertilizer supply. The mutation also changed the distribution of N between the vegetative and reproductive organs. Our findings suggest that the transcription factor OsSHR2 plays a regulatory role in the response of plant architecture and yield per plant to N in rice.
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Data availability
No data were used for the study described in the article.
Abbreviations
- SHR:
-
Shortroot
- SLR:
-
Slender rice
- DLT:
-
Dwarf and low-tillering
- N:
-
Nitrogen
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Acknowledgements
This work was partially supported by the Sanya Yazhou Bay Science and Technology City (SCKJ-JYRC-2023-71), National Key Research and Development Program of China (2021YFF1000404) and National Natural Science Foundation of China (42107428). We would like to thanks to Professor Chuanzao Mao for providing access to the Zhejiang University Agricultural Experimental Station, Changxing County, Zhejiang Province, China, where we planted the experimental materials.
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SSB and HZ conceived and designed research. HZ, XHH, HX and LHX conducted experiments. WXW, XGH and SSB contributed to the resources and supervision. YSS, HX, CMY and SYS analyzed data. HZ and SSB wrote the manuscript. SSB, HZ, SK, HX, XHH and LHX modified the manuscript. All authors read and approved the manuscript.
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Hu, Z., Huang, X., Xia, H. et al. Transcription factor OsSHR2 regulates rice architecture and yield per plant in response to nitrogen. Planta 259, 148 (2024). https://doi.org/10.1007/s00425-024-04400-7
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DOI: https://doi.org/10.1007/s00425-024-04400-7