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Chromosomal mapping of a locus associated with adult-stage resistance to powdery mildew from Agropyron cristatum chromosome 6PL in wheat

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Abstract

Key message

The powdery mildew resistance locus was mapped to A. cristatum chromosome 6PL bin (0.27–0.51) and agronomic traits evaluation indicated that this locus has potential breeding application value.

Abstract

Agropyron cristatum (2n = 4x = 28, PPPP) is a wild relative of wheat with an abundance of biotic and abiotic stress resistance genes and is considered one of the best exogenous donor relatives for wheat breeding. A number of wheat-A. cristatum derived lines have been generated, including addition lines, translocation lines and deletion lines. In this study, the 6P disomic addition line 4844-12 (2n = 2x = 44) was confirmed to have genetic effects on powdery mildew resistance. Four 6P deletion lines (del16a, del19b, del21 and del27) and two translocation lines (WAT638a and WAT638b), derived from radiation treatment of 4844-12, were used to further assess the 6P powdery mildew resistance locus by powdery mildew resistance assessment, genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH) and 6P specific sequence-tagged-site (STS) markers. Collectively, the locus harboring the powdery mildew resistance gene was genetically mapped to a 6PL bin (0.27–0.51). The genetic effects of this chromosome segment on resistance to powdery mildew were further confirmed by del16a and del27 BC3F2 lines. Comprehensive evaluation of agronomic traits revealed that the powdery mildew resistance locus of 6PL (0.27–0.51) has potential application value in wheat breeding. A total of 22 resistant genes were annotated and 3 specific gene markers were developed for detecting chromatin of the resistant region based on genome re-sequencing. In summary, this study could broaden the powdery mildew resistance gene pool for wheat genetic improvements.

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Acknowledgements

Thanks to Wheat Resource Center, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences.

Funding

This research was funded by National Science Foundation of China (31801359) and the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2021-ICS). National Aerospace Science Foundation of China, 31801359, Shenghui Zhou.

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, 712100, China

    Yida Lin & Lihui Li

  2. National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Yida Lin, Shenghui Zhou, Xuezhong Liang, Baojin Guo, Bing Han, Haiming Han, Jingpeng Zhang, Yuqing Lu, Zhi Zhang, Xinming Yang, Xiuquan Li, Weihua Liu & Lihui Li

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  1. Yida Lin
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  2. Shenghui Zhou
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  3. Xuezhong Liang
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  4. Baojin Guo
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  5. Bing Han
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  12. Weihua Liu
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  13. Lihui Li
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Contributions

LHL conceived the research. YDL performed the research. YDL and SHZ wrote the paper. BH and XZL participated in part of cytology work. BJG, HMH, JPZ, YQL, ZZ, XMY, XQL and WHL participated in the preparation of the reagents and materials used in this study.

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Correspondence to Lihui Li.

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Communicated by Reem Aboukhaddour.

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Yida Lin and Shenghui Zhou have contributed equally to this work.

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Lin, Y., Zhou, S., Liang, X. et al. Chromosomal mapping of a locus associated with adult-stage resistance to powdery mildew from Agropyron cristatum chromosome 6PL in wheat. Theor Appl Genet 135, 2861–2873 (2022). https://doi.org/10.1007/s00122-022-04155-3

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