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Mapping of novel powdery mildew resistance gene(s) from Agropyron cristatum chromosome 2P

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Abstract

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A physical map of Agropyron cristatum 2P chromosome was constructed for the first time and the novel powdery mildew resistance gene(s) from chromosome 2P was(were) also mapped.

Abstract

Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), a wild relative of common wheat, is highly resistant to powdery mildew. Previous studies showed that wheat-A. cristatum 2P disomic addition line II-9-3 displayed high resistance to powdery mildew, and the resistance was attributable to A. cristatum chromosome 2P. To utilize and physically map the powdery mildew resistance gene(s), 15 wheat-A. cristatum 2P translocation lines and three A. cristatum 2P deletion lines with different chromosomal segment sizes, obtained from II-9-3 using 60Co-γ ray irradiation, were characterized using cytogenetic and molecular marker analysis. A. cristatum 2P chromosomal segments in the translocations were translocated to different wheat chromosomes, including 1A, 4A, 5A, 6A, 7A, 1B, 2B, 3B, 7B, 3D, 4D, and 6D. A physical map of the 2P chromosome was constructed with 82 STS markers, consisting of nine bins with 34 markers on 2PS and eight bins with 48 markers on 2PL. The BC1F2 populations of seven wheat-A. cristatum 2P translocation lines (2PT-3, 2PT-4, 2PT-5, 2PT-6, 2PT-8, 2PT-9, and 2PT-10) were developed by self-pollination, tested with powdery mildew and genotyped with 2P-specific STS markers. From these results, the gene(s) conferring powdery mildew resistance was(were) located on 2PL bin FL 0.66–0.86 and 19 2P-specific markers were identified in this bin. Moreover, two new powdery mildew-resistant translocation lines (2PT-4 and 2PT-5) with small 2PL chromosome segments were obtained. The newly developed wheat lines with powdery mildew resistance and the closely linked molecular markers will be valuable for wheat disease breeding in the future.

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Acknowledgments

We would like to thank Dr. Hongjie Li (Institute of Crop Science, Chinese Academy of Agricultural Sciences) for providing Bgt isolates E09 and E20. This research was supported by Grants from the National Natural Science Foundation of China (Grant No. 31471493 and 31271714), the National High Technology Research and Development Program of China (863 Grant No. 2011AA100101) and the National Key Technology Support Program of China (Grant No. 2013BAD01B02).

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  1. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Huanhuan Li, Bo Jiang, Yuqing Lu, Jinpeng Zhang, Cuili Pan, Xinming Yang, Xiuquan Li, Weihua Liu & Lihui Li

  2. Baoji Academy of Agricultural Sciences, Baoji, 722499, China

    Jingchang Wang

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  1. Huanhuan Li
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Li, H., Jiang, B., Wang, J. et al. Mapping of novel powdery mildew resistance gene(s) from Agropyron cristatum chromosome 2P. Theor Appl Genet 130, 109–121 (2017). https://doi.org/10.1007/s00122-016-2797-9

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