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Introgression of genes from bread wheat enhances the aluminium tolerance of durum wheat

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The aluminium tolerance of durum wheat was markedly enhanced by introgression of TaALMT1 and TaMATE1B from bread wheat. In contrast to bread wheat, TaMATE1B conferred greater aluminium tolerance than TaALMT1.

Abstract

Durum wheat (tetraploid AABB, Triticum turgidum) is a species that grows poorly on acid soils due to its sensitivity of Al3+. By contrast, bread wheat (hexaploid AABBDD, T. aestivum) shows a large variation in Al3+ tolerance which can be attributed to a major gene (TaALMT1) located on chromosome 4D as well as to other genes of minor effect such as TaMATE1B. Genotypic variation for Al3+ tolerance in durum germplasm is small and the introgression of genes from bread wheat is one option for enhancing the ability of durum wheat to grow on acid soils. Introgression of a large fragment of the 4D chromosome previously increased the Al3+ tolerance of durum wheat demonstrating the viability of transferring the TaALMT1 gene to durum wheat to increase its Al3+ tolerance. Here, we used a ph1 (pairing homoeologous) mutant of durum wheat to introgress a small fragment of the 4D chromosome harboring the TaALMT1 gene. The size of the 4D chromosomal fragment introgressed into durum wheat was estimated by markers, fluorescence in situ hybridisation and real-time quantitative PCR. In a parallel strategy, we introgressed TaMATE1B from bread wheat into durum wheat using conventional crosses. Both genes separately increased the Al3+ tolerance of durum wheat in both hydroponics and soil cultures. In contrast to bread wheat, the TaMATE1B gene was more effective than TaALMT1 in increasing the Al3+ tolerance of durum wheat grown on acid soil.

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Acknowledgments

C.H. thanks the China Scholarship Council for financial support of a studentship.

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Author notes

  1. Chang Han

    Present address: School of Agriculture and Technology, Zunyi Normal College, Zunyi, 563002, Guizhou, China

Authors and Affiliations

  1. CSIRO Agriculture, GPO Box 1600, Canberra, ACT, 2601, Australia

    Chang Han, Peter R. Ryan, Tina M. Rathjen & Emmanuel Delhaize

  2. The University of Sydney, 107 Cobbitty Rd, Cobbitty, NSW, 2570, Australia

    Peng Zhang

  3. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China

    ZeHong Yan

Authors
  1. Chang Han
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  2. Peng Zhang
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  6. Emmanuel Delhaize
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Correspondence to Emmanuel Delhaize.

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Communicated by X. Xia.

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122_2015_2661_MOESM1_ESM.docx

Supplementary material 1 (DOCX 12 kb) Supplementary Fig. S1. Relative quantity of D-genome in the TaMATE1B line as estimated by a RT-qPCR assay of the Dgas family of sequences. The Dgas sequence is expressed as a ratio of the 28S ribosomal RNA sequences with the data normalized to show the value of a durum line that has the long arm of chromosome 4D (LF line) set at 1.0. The durum wheat cv Jandaroi was used as a negative control for the D-genome. Error bars show the SE with n = 3. Bars with different letters denote statistically different values at P < 0.05 as determined with a one-way ANOVA

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Han, C., Zhang, P., Ryan, P.R. et al. Introgression of genes from bread wheat enhances the aluminium tolerance of durum wheat. Theor Appl Genet 129, 729–739 (2016). https://doi.org/10.1007/s00122-015-2661-3

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  • DOI: https://doi.org/10.1007/s00122-015-2661-3

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