Skip to main content
SpringerLink
Log in

Identification and characterization of pleiotropic and co-located resistance loci to leaf rust and stripe rust in bread wheat cultivar Sujata

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Key message

Two new co-located resistance loci, QLr.cim - 1AS/QYr.cim - 1AS and QLr.cim - 7BL/YrSuj , in combination with Lr46 / Yr29 and Lr67/Yr46 , and a new leaf rust resistance quantitative trait loci, conferred high resistance to rusts in adult plant stage.

Absract

The tall Indian bread wheat cultivar Sujata displays high and low infection types to leaf rust and stripe rust, respectively, at the seedling stage in greenhouse tests. It was also highly resistant to both rusts at adult plant stage in field trials in Mexico. The genetic basis of this resistance was investigated in a population of 148 F5 recombinant inbred lines (RILs) derived from the cross Avocet × Sujata. The parents and RIL population were characterized in field trials for resistance to leaf rust during 2011 at El Batán, and 2012 and 2013 at Ciudad Obregón, Mexico, and for stripe rust during 2011 and 2012 at Toluca, Mexico; they were also characterized three times for stripe rust at seedling stage in the greenhouse. The RILs were genotyped with diversity arrays technology and simple sequence repeat markers. The final genetic map was constructed with 673 polymorphic markers. Inclusive composite interval mapping analysis detected two new significant co-located resistance loci, QLr.cim-1AS/QYr.cim-1AS and QLr.cim-7BL/YrSuj, on chromosomes 1AS and 7BL, respectively. The chromosomal position of QLr.cim-7BL overlapped with the seedling stripe rust resistance gene, temporarily designated as YrSuj. Two previously reported pleiotropic adult plant resistance genes, Lr46/Yr29 and Lr67/Yr46, and a new leaf rust resistance quantitative trait loci derived from Avocet were also mapped in the population. The two new co-located resistance loci are expected to contribute to breeding durable rust resistance in wheat. Closely linked molecular markers can be used to transfer all four resistance loci simultaneously to modern wheat varieties.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Bariana HS, Bansal UK, Schmidt A, Lehmensiek A, Kaur J, Miah H, Howes N, McIntyre CL (2010) Molecular mapping of adult plant stripe rust resistance in wheat and identification of pyramided QTL genotypes. Euphytica 176:251–260

    Article  CAS  Google Scholar 

  • Basnet BR, Singh RP, Herrera-Foessel SA, Ibrahim AMH, Huerta-Espino J, Calvo-Salazar V, Rudd J (2013) Genetic analysis of adult plant resistance to yellow rust and leaf rust in common spring wheat Quaiu#3. Plant Dis 97:728–736

    Article  CAS  Google Scholar 

  • Basnet BR, Singh RP, Ibrahim AMH, Herrera-Foessel SA, Huerta-Espino J, Lan CX, Rudd JC (2014) Characterization of Yr54 and other genes associated with adult plant resistance to yellow rust and leaf rust in common wheat Quaiu 3. Mol Breed 33:385–399

    Article  CAS  Google Scholar 

  • Bjarko ME, Line RF (1988) Heritability and number of genes controlling leaf rust resistance in four cultivars of wheat. Phytopathology 78:457–461

    Article  Google Scholar 

  • Chen XM (2005) Epidemiology and control of stripe rust (Puccinia striiformis f. sp. tritici) on wheat. Can J Plant Pathol 27:314–337

    Article  Google Scholar 

  • Chen XM (2013) Review article: High-temperature adult-plant resistance, key for sustainable control of stripe rust. Am J Plant Sci 4:608–627

    Article  Google Scholar 

  • Chu CG, Friesen TL, Xu SS, Faris JD, Kolmer JA (2009) Identification of novel QTLs for seedling and adult plant leaf rust resistance in a wheat doubled haploid population. Theor Appl Genet 119:263–269

    Article  PubMed  Google Scholar 

  • CIMMYT (2005) Laboratory protocols: CIMMYT Applied Molecular Genetics Laboratory, 3rd edn. CIMMYT, Mexico

    Google Scholar 

  • Das MK, Rajaram S, Mundt CC, Kronstad WE, Singh RP (1992) Inheritance of slow rusting resistance in wheat. Crop Sci 32:1452–1456

    Article  Google Scholar 

  • Dedryver F, Paillard S, Mallard S, Robert O, Trottet M, Nègre S, Verplancke G, Jahier J (2009) Characterization of genetic components involved in durable resistance to stripe rust in the bread wheat ‘Renan’. Phytopathology 99:968–973

    Article  CAS  PubMed  Google Scholar 

  • Dubin HJ, Brennan JP (2009) Fighting a ‘shifty enemy’. In: The international collaboration to contain wheat rusts, chapter 2, pp 19–24 (book)

  • Dyck PL, Samborski DJ (1979) Adult plant resistance in PI250413, an introduction of common wheat. Can J Plant Sci 59:329–332

    Article  Google Scholar 

  • Faris JD, Li WL, Liu DJ, Chen PD, Gill BS (1999) Candidate gene analysis of quantitative disease resistance in wheat. Theor Appl Genet 98:219–225

    Article  CAS  Google Scholar 

  • Feuillet C, Travella S, Stein N, Albar L, Nublat A, Keller B (2003) Map-based isolation of the leaf rust disease resistance gene Lr10 from the hexaploid wheat (Triticum aestivum L.) genome. Proc Natl Acad Sci 100:15253–15258

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Flor HH (1942) Inheritance of pathogenicity in Melampsora lini. Phytopathology 32:653–669

    Google Scholar 

  • Francki MG, Walker E, Crawford AC, Broughton S, Ohm HW, Barclay I, Wilson RE, McLean R (2009) Comparison of genetic and cytogenetic maps of hexaploid wheat (Triticum aestivum L.) using SSR and DArT markers. Mol Genet Genomics 281:181–191

    Article  CAS  PubMed  Google Scholar 

  • Fu DL, Uauy C, Distelfeld A, Blechl A, Epstein L, Chen XM, Sela H, Fahima T, Dubcovsky J (2009) A Kinase-START gene confers temperature-dependent resistance to wheat stripe rust. Science 323:1357–1359

    Article  CAS  PubMed  Google Scholar 

  • Hasabnis SN, Joi MB, Shinde VK, Ilhe BM (2003) Adult plant resistance of wheat varieties against leaf rust (Puccinia recondita f. sp. tritici). Agric Sci Dig 23:6–9

    Google Scholar 

  • Herrera-Foessel SA, Singh RP, Huerta-Espino J, Rosewarne GM, Periyannan SK, Viccars L, Calvo-Salazar V, Lan CX, Lagudah ES (2012) Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor Appl Genet 124:1475–1486

    Article  CAS  PubMed  Google Scholar 

  • Herrera-Foessel SA, Singh RP, Lillemo M, Huerta-Espino J, Bhavani S, Singh S, Lan CX, Calvo-Salazar V, Lagudah ES (2014) Lr67/Yr46 confers adult plant resistance to stem rust and powdery mildew in wheat. Theor Appl Genet 127:781–789

    Article  CAS  PubMed  Google Scholar 

  • Huang BE, George AW, Forrest KL, Kilian A, Hayden MJ, Morell MK, Cavanagh CR (2012) A multiparent advanced generation inter-cross population for genetic analysis in wheat. Plant Biol J 10:826–839

    CAS  Google Scholar 

  • Johnson R, Law CN (1973) Cytogenetic studies on the resistance of the wheat variety Bersée to Puccinia striiformis. Cereal Rusts Bull 1:38–43

    Google Scholar 

  • Khan M, Bukhari A, Dar Z, Rizvi S (2013) Status and strategies in breeding for rust resistance in wheat. Agric Sci 4:292–301

    Google Scholar 

  • Knott DR, Padidam M (1988) Inheritance of resistance to stem rust in six wheat lines having adult plant resistance. Genome 30:283–288

    Article  Google Scholar 

  • Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1362

    Article  CAS  PubMed  Google Scholar 

  • Lan CX, Rosewarne GM, Singh RP, Herrera-Foessel SA, Huerta-Espino J, Basnet BR, Zhang YL, Yang EN (2014) QTL characterization of resistance to leaf rust and stripe rust in the spring wheat line Francolin#1. Mol Breed 34:789–803

    Article  CAS  Google Scholar 

  • Li ZF, Lan CX, He ZH, Singh RP, Rosewarne GM, Chen XM, Xia XC (2014) Overview and application of QTL for adult plant resistance to leaf rust and powdery mildew in wheat. Crop Sci 54:1907–1925

    Article  Google Scholar 

  • Lillemo M, Asalf B, Singh RP, Huerta-Espino J, Chen XM, He ZH, Bjørnstad A (2008) The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet 116:1155–1166

    Article  CAS  PubMed  Google Scholar 

  • Lin F, Chen XM (2007) Genetics and molecular mapping of genes for race-specific all-stage resistance and non-race-specific high-temperature adult-plant resistance to stripe rust in spring wheat cultivar Alpowa. Theor Appl Genet 114:1277–1287

    Article  CAS  PubMed  Google Scholar 

  • McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO, Australia

  • McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2013) Catalogue of gene symbols for wheat: 2013 supplement. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/2013/GeneCatalogueIntroduction.pdf

  • McNeal FH, Konzak CF, Smith EP, Tate WS, Russell TS (1971) A uniform system for recording and processing cereal research data, pp 34–121. USDA-ARS Bull, Washington

  • Messmer MM, Seyfarth R, Keller M, Schachermayr G, Winzeler M, Zanetti S, Feuillet C, Keller B (2000) Genetic analysis of durable leaf rust resistance in winter wheat. Theor Appl Genet 100:419–431

    Article  CAS  Google Scholar 

  • Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res 26:496–500

    Article  Google Scholar 

  • Ren Y, He ZH, Li J, Lillemo M, Wu L, Bai B, Lu QX, Zhu HZ, Zhou G, Du JY, Lu QL, Xia XC (2012a) QTL mapping of adult-plant resistance to stripe rust in a population derived from common wheat cultivars Naxos and Shanghai 3/Catbird. Theor Appl Genet 125:1211–1221

    Article  PubMed  Google Scholar 

  • Ren Y, Li ZF, He ZH, Wu L, Bai B, Lan CX, Wang CF, Zhou G, Zhu HZ, Xia XC (2012b) QTL mapping of adult-plant resistances to stripe rust and leaf rust in Chinese wheat cultivar Bainong 64. Theor Appl Genet 125:1253–1262

    Article  CAS  PubMed  Google Scholar 

  • Ren RS, Wang MN, Chen XM, Zhang ZJ (2012c) Characterization and molecular mapping of Yr52 for high-temperature adult-plant resistance to stripe rust in spring wheat germplasm PI 183527. Theor Appl Genet 125:847–857

    Article  CAS  PubMed  Google Scholar 

  • Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. CIMMYT, Mexico

  • Rosewarne GM, Singh RP, Huerta-Espino J, William HM, Bouchet S, Cloutier S, McFadden H, Lagudah ES (2006) Leaf tip necrosis, molecular markers and β1-proteasome subunits associated with the slow rusting resistance genes Lr46/Yr29. Theor Appl Genet 112:500–508

    Article  CAS  PubMed  Google Scholar 

  • Rosewarne GM, Singh RP, Huerta-Espino J, Rebetzke GJ (2008) Quantitative trait loci for slow-rusting resistance in wheat to leaf rust and stripe rust identified with multi-environment analysis. Theor Appl Genet 116:1027–1034

    Article  CAS  PubMed  Google Scholar 

  • Rosewarne GM, Singh RP, Huerta-Espino J, Herrera-Foessel SA, Forrest KL, Hayden MJ, Rebetzke GJ (2012) Analysis of leaf and stripe rust severities reveals pathotype changes and multiple minor QTLs associated with resistance in an Avocet × Pastor wheat population. Theor Appl Genet 124:1283–1294

    Article  CAS  PubMed  Google Scholar 

  • Rosewarne GM, Herrera-Foessel SA, Singh RP, Huerta-Espino J, Lan CX, He ZH (2013) Quantitative trait loci of stripe rust resistance in wheat. Theor Appl Genet 126:2427–2449

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Schnurbusch T, Paillard S, Schori A, Messmer M, Schachermayr G, Winzeler M, Keller B (2004) Dissection of quantitative and durable leaf rust resistance in Swiss winter wheat reveals a major resistance QTL in the Lr34 chromosome region. Theor Appl Genet 108:477–484

    Article  CAS  PubMed  Google Scholar 

  • Singh RP, Rajaram S (1992) Genetics of adult-plant resistance to leaf rust in ‘Frontana’ and three CIMMYT wheats. Genome 35:24–31

    Article  Google Scholar 

  • Singh RP, Mujeeb-Kazi A, Huerta-Espino J (1998) Lr46: a gene conferring slow rusting resistance to leaf rust in wheat. Phytopathology 88:890–894

    Article  CAS  PubMed  Google Scholar 

  • Singh RP, Nelson JC, Sorrells ME (2000) Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci 44:1148–1155

    Article  Google Scholar 

  • Singh RP, Herrera-Foessel SA, Huerta-Espino J, Bariana H, Bansal U, McCallum B, Hiebert C, Bhavani S, Singh S, Lan CX, Lagudah ES (2012) Lr34/Yr18/Sr57/Pm38/Bdv1/Ltn1 confers slow rusting, adult plant resistance to Puccinia graminis tritici. In: Chen W-Q (ed) Proceedings of the 13th international cereal rusts and powdery mildews conference, Beijing

  • Singh RP, Herrera-Foessel SA, Huerta-Espino J, Lan CX, Basnet BR, Bhavani S, Lagudah ES (2013) Pleiotropic gene Lr46/Yr29/Pm39/Ltn2 confers slow rusting, adult plant resistance to wheat stem rust fungus. In: Proceedings Borlaug global rust initiative, 2013 technical workshop, New Delhi, p 17.1, 19–22 August 2013

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  CAS  PubMed  Google Scholar 

  • Suenaga K, Singh RP, Huerta-Espino J, William HM (2003) Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology 93:881–890

    Article  CAS  PubMed  Google Scholar 

  • Van Ooijen JW (2006) Join Map 4, software for the calculation of genetic linkage maps in experimental population. Plant Research International, Wageningen

    Google Scholar 

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. Heredity 93:77–78

    Article  CAS  Google Scholar 

  • Wan AM, Chen XM, He ZH (2007) Wheat stripe rust in China. Aust J Agric Res 58:605–619

    Article  Google Scholar 

  • Wellings CR (2011) Global status of stripe rust: a review of historical and current threats. Euphytica 179:129–141

    Article  Google Scholar 

  • Wilkinson PA, Winfield MO, Barker GLA, Allen AM, Burridge A, Coghill JA, Burridge A, Edwards KJ (2012) CerealsDB 2.0: an integrated resource for plant breeders and scientists. BMC Biol 13:219

  • William HM, Singh RP, Huerta-Espino J, Palacios G, Suenaga K (2006) Characterization of genetic loci conferring adult plant resistance to leaf rust and stripe rust in spring wheat. Genome 49:977–990

    Article  CAS  PubMed  Google Scholar 

  • Wright S (1968) Evolution and the genetics of populations, vol 1. In: Genetic and biometric foundations. University of Chicago Press, Chicago

  • Xu HX, Zhang J, Zhang P, Qie YM, Niu YC, Li HJ, Ma PT, Xu YF, An DG (2014) Development and validation of molecular markers closely-linked to the wheat stripe rust resistance gene YrC591 for marker-assisted selection. Euphytica 198:317–323

    Article  CAS  Google Scholar 

  • Yang EN, Rosewarne GM, Herrera-Foessel SA, Huerta-Espino J, Tang ZX, Sun CF, Ren ZL, Singh RP (2013) QTL analysis of the spring wheat ‘Chapio’ identifies stable stripe rust resistance despite inter-continental genotype × environment interactions. Theor Appl Genet 126:1721–1732

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We thank the Grains Research and Development Corporation (GRDC) of Australia and CIMMYT, SAGARPA-CONACYT of Mexico (Fondo Sectorial project 146788) for their financial support. Technical editing by Alma McNab and Mike Listman is greatly appreciated.

Conflict of interest

No conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication.

Ethical standards

I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously, and is not under consideration for publication elsewhere. All the authors listed have approved the manuscript.

Author information

Authors and Affiliations

  1. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico, DF, Mexico

    Caixia Lan, Sybil A. Herrera-Foessel, Bhoja R. Basnet & Ravi P. Singh

  2. Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences/Hebei Laboratory of Crop Genetics and Breeding, Shijiazhuang, 050031, Hebei, People’s Republic of China

    Yelun Zhang

  3. Campo Experimental Valle de México INIFAP, Apdo. Postal 10, 56230, Chapingo, State of Mexico, Mexico

    Julio Huerta-Espino

  4. CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia

    Evans S. Lagudah

Authors
  1. Caixia Lan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yelun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sybil A. Herrera-Foessel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bhoja R. Basnet
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Julio Huerta-Espino
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Evans S. Lagudah
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ravi P. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ravi P. Singh.

Additional information

Communicated by Beat Keller.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lan, C., Zhang, Y., Herrera-Foessel, S.A. et al. Identification and characterization of pleiotropic and co-located resistance loci to leaf rust and stripe rust in bread wheat cultivar Sujata. Theor Appl Genet 128, 549–561 (2015). https://doi.org/10.1007/s00122-015-2454-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-015-2454-8

Keywords

Search

Navigation