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Identification of novel genomic regions associated with resistance to Pyrenophora tritici-repentis races 1 and 5 in spring wheat landraces using association analysis

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Abstract

Tan spot, caused by Pyrenophora tritici-repentis, is a major foliar disease of wheat worldwide. Host plant resistance is the best strategy to manage this disease. Traditionally, bi-parental mapping populations have been used to identify and map quantitative trait loci (QTL) affecting tan spot resistance in wheat. The association mapping (AM) could be an alternative approach to identify QTL based on linkage disequilibrium (LD) within a diverse germplasm set. In this study, we assessed resistance to P. tritici-repentis races 1 and 5 in 567 spring wheat landraces from the USDA-ARS National Small Grains Collection (NSGC). Using 832 diversity array technology (DArT) markers, QTL for resistance to P. tritici-repentis races 1 and 5 were identified. A linear model with principal components suggests that at least seven and three DArT markers were significantly associated with resistance to P. tritici-repentis races 1 and 5, respectively. The DArT markers associated with resistance to race 1 were detected on chromosomes 1D, 2A, 2B, 2D, 4A, 5B, and 7D and explained 1.3–3.1% of the phenotypic variance, while markers associated with resistance to race 5 were distributed on 2D, 6A and 7D, and explained 2.2–5.9% of the phenotypic variance. Some of the genomic regions identified in this study correspond to previously identified loci responsible for resistance to P. tritici-repentis, offering validation for our AM approach. Other regions identified were novel and could possess genes useful for resistance breeding. Some DArT markers associated with resistance to race 1 also were localized in the same regions of wheat chromosomes where QTL for resistance to yellow rust, leaf rust and powdery mildew, have been mapped previously. This study demonstrates that AM can be a useful approach to identify and map novel genomic regions involved in resistance to P. tritici-repentis.

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Acknowledgments

The authors gratefully acknowledge financial support for this project from the Wheat Research and Promotion Council, Minnesota, North Dakota Wheat Commission, and State Board of Agricultural Research and Education, North Dakota, and USDA-ARS specific cooperative agreement 58-5366-0-133. We are grateful to Dr. Andrzej Kilian for DArT marker data analysis and Jana Hansen for technical help.

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  1. S. Gurung

    Present address: Department of Plant Pathology, University of California, Davis, c/o U.S. Agricultural Research Station, 1636 E. Alisal Street, Salinas, CA, 93905, USA

Authors and Affiliations

  1. Department of Plant Pathology, North Dakota State University, NDSU Dept. 7660, P.O. Box 6050, Fargo, ND, 58108-6050, USA

    S. Gurung, L. E. del Río, M. Acevedo & T. B. Adhikari

  2. Department of Plant Sciences, North Dakota State University, NDSU Dept. #7670, PO Box 6050, Fargo, ND, 58108-6050, USA

    S. Mamidi & M. Mergoum

  3. Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID, 83210, USA

    J. M. Bonman & E. W. Jackson

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Correspondence to T. B. Adhikari.

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Communicated by B. Keller.

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Gurung, S., Mamidi, S., Bonman, J.M. et al. Identification of novel genomic regions associated with resistance to Pyrenophora tritici-repentis races 1 and 5 in spring wheat landraces using association analysis. Theor Appl Genet 123, 1029–1041 (2011). https://doi.org/10.1007/s00122-011-1645-1

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