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Molecular mapping of an apical branching gene of cultivated sunflower (Helianthus annuus L.)

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Abstract

Commercial hybrids of cultivated sunflower (Helianthus annuus L.) are obtained by crossing a cytoplasmic male sterile line (A-line) with a restorer pollinator (R-line). The incorporation of a recessive branching trait to extend the pollination period of R-lines during hybrid seed production is laborious and time-consuming. By using target region polymorphism (TRAP) and bulked segregant analysis (BSA), we identified 15 TRAP markers linked to the b 1 (branching) locus in a population of 229 F2 plants derived from a cross between nonbranched (HA 234) and branched (RHA 271) lines. TBr4-720 and TBr8-555 markers were linked to the b 1 gene in the coupling phase at 0.5 cM (0.004 recombination frequency). The Tbr20-297 and Tbr20-494 markers flanked the b 1 locus in the repulsion phase at genetic distances of 7.5 and 2.5 cM, respectively. Tbr19-395, also in the repulsion phase, mapped at 3.8 cM from the b 1 locus and on the opposite side of the marker Tbr20-297. The 8A1 and 15B3 restriction fragment length polymorphic (RFLP) markers of linkage group (LG) 16 of the RHA 271 × HA 234 cultivated sunflower map anchored the b 1 LG onto the RFLP map. Polymerase chain reaction (PCR)-based markers tightly linked to the recessive b 1 gene have been developed. Their identification and the incorporation of the LG containing the b 1 locus onto an RFLP map will be useful for marker-assisted selection (MAS) in breeding programs and provide the bases for map-based cloning of this gene.

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Acknowledgments

This work was supported by a postdoctoral grant to P.R.B. from the Spanish Secretary of Education and Universities, and the European Social Fund. The authors thank Dr. Jose Luis Gonzalez and Dr. Dennis J. Tobias for their assistance, valuable advice and suggestions throughout this work. Authors also thank Lisa Brown and Angelia Hogness for their technical assistance in the greenhouse and laboratory.

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  1. Pilar Rojas-Barros

    Present address: Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, St. Paul, MN, 55108, USA

Authors and Affiliations

  1. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58105, USA

    Pilar Rojas-Barros

  2. US Department of Agriculture, Agricultural Research Service, Northern Crop Science Laboratory, PO Box 5677, Fargo, ND, 58105, USA

    Jinguo Hu & C. C. Jan

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  1. Pilar Rojas-Barros
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  2. Jinguo Hu
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Correspondence to C. C. Jan.

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Communicated by M. Xu.

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Rojas-Barros, P., Hu, J. & Jan, C.C. Molecular mapping of an apical branching gene of cultivated sunflower (Helianthus annuus L.). Theor Appl Genet 117, 19–28 (2008). https://doi.org/10.1007/s00122-008-0748-9

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