Skip to main content
SpringerLink
Log in

RFLPs of the rRNA genes in the genusPhaseolus

  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Restriction fragment length polymorphisms of the rRNA genes were investigated among 20 genotypes ofP. vulgaris, P. coccineus, P. polyanthus, P. microcarpus, P. glabellus, P. acutifolius, P. maculatus, P. oligospermus andP. lunatus. Detection of the polymorphisms was performed nonradioactively with a digoxigenin-labeled rDNA probe. RFLP-based phylogenetic trees for the rDNA of the species studied were computed with several distance matrix and parsimony methods. The estimated molecular relationships within the genusPhaseolus coincide, on principle, with the classical taxonomical investigations. Hence, RFLP analyses of the rRDA have been proven useful for systematic studies in the genusPhaseolus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Appels, R. & R. L. Honeycutt, 1986. rDNA: Evolution over a billion years. In: S. K. Dutta (Ed.), DNA systematics, Vol. 2, pp. 81–135, CRC Press, Boca Raton, USA.

    Google Scholar 

  • Baudoin, J. P. & K. Katanga, 1990. Phyletic relationships within the genusPhaseolus on basis of pollen morphology and experimental hybridization. Ann. Rep. Bean Improv. Coop. 33: 117–118.

    Google Scholar 

  • Baudoin, J. P., Schmit, V. & B. Wathelet, 1991. Observation on seed protein electrophoretic patterns of some species within the genusPhaseolus. Ann. Rep. Bean Improv. Coop. 34: 85–87.

    Google Scholar 

  • Benedetelli, S., Taurchini, D. & R. D'Ovidio, 1992. Ribosomal DNA structure inCastanea spp. (Fagaceae) and their hybrids. J. Genet. Breed. 46: 57–62.

    Google Scholar 

  • Berglund-Brücher, O. & H. Brücher, 1976. The South American wild bean (Phaseolus aborigineus BURK.) as ancestor of the common bean. Econ. Bot. 30: 257–272.

    Google Scholar 

  • Brücher, H., 1988. The wild ancestor ofPhaseolus vulgaris in South American. In: P. Gepts (Ed.), Genetic resource ofPhaseolus beans, pp. 185–214, Kluwer Academic Publishers, Dordrecht, The Netherlands.

    Google Scholar 

  • Bukasov, S. M., 1931. The cultivated plants of Mexico, Guatemala and Columbia. Bull. Appl. Bet. Genet. Plant Breed., Leningrad 47:450–553.

    Google Scholar 

  • Camarena, M. F. & J. P. Baudoin, 1987. Obtention des premiers hybrides interspécifiques entrePhaseolus vulgaris etPhaseolus polyanthus avec le cytoplasme de cette dernière forme. Bull. Rech. Agron. Gembloux 22:43–45.

    Google Scholar 

  • Debouck, D. G., 1988.Phaseolus germplasm exploration. In: P. Gepts (Ed.), Genetic resources ofPhaseolus beans, pp. 3–29, Kluwer Academic Publishers, Dordrecht, The Netherlands.

    Google Scholar 

  • Debouck, D. G., 1991. Systematics and morphology. In: A. van Schoonhoven & O. Voysest (Eds.), Common beans: research for crop improvement, pp. 55–118, Commonwealth Agricultural Bureaux International, Wallingford, United Kingdom.

    Google Scholar 

  • Debouck, D. G., 1992. Views on variability inPhaseolus beans. Ann. Rep. Bean Improv. Coop. 35: 9–10.

    Google Scholar 

  • Debouck, D. G., Maquet, A. & C. E. Posso, 1989. Biochemical evidence for two different gene pools in Lima beans,Phaseolus lunatus L. Ann. Rep. Bean Improv. Coop. 32: 58–59.

    Google Scholar 

  • Debouck, D. G., Schmit, V., Libreros, D. & H. Ramirez, 1990. Biochemical evidence for a fifth cultigen within the genusPhaseolus. Ann. Rep. Bean Improv. Coop. 33: 106–107.

    Google Scholar 

  • Delgado Salinas, A., 1988. Variation, taxonomy, domestication and germplasm potentialities inP. coccineus. In: P. Gepts (Ed.), Genetic resources ofPhaseolus beans, pp. 441–463, Kluwer Academic Publishers, Dordrecht, The Netherlands.

    Google Scholar 

  • Dellaporta, S. L., Wood, J. & J. B. Hicks, 1984. Maize-miniprep. DNA isolation. In: R. Malmberg, J. Messing & I. Sussex (Eds.), Molecular biology of plants — a laboratory course manual, pp. 36–37, Cold Spring Harbor Laboratory, New York; USA.

    Google Scholar 

  • Doyle, J. J., 1993. DNA, phylogeny, and the flowering of plant systematics. BioScience 43: 380–389.

    Article  Google Scholar 

  • Düring, K., 1991. Ultrasensitive chemiluminescent and colorigenic detection of DNA, RNA, and proteins in plant molecular biology. Anal. Biochem. 196: 433–438.

    Article  PubMed  Google Scholar 

  • Evans, A. M., 1980. Structure, variation, evolution and classification inPhaseolus. In: R. J. Summerfield & A. H. Bunting (Eds.), Advances in legume science, pp. 337–347. Royal Botanic Gardens, Kew, England.

    Google Scholar 

  • Farris, J. S., 1977. Phylogenetic analysis under Dollo's law. Syst. Zool. 26: 77–88.

    Article  Google Scholar 

  • Feinberg, A. P. & B. Vogelstein, 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132: 6–18.

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein, J., 1988. Phylogenies from molecular sequences: inference and reliability. Annu. Rev. Genet. 22: 521–565.

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein, J., 1989. PHYLIP (Phylogeny Inference Package) Version 3.2. Cladistics 5: 164–166.

    Google Scholar 

  • Flavell, R. B., O'Dell, M., Sharp, P., Nevo, E. & A. Beiles, 1986. Variation in the intergenic spacer of ribosomal DNA of wild wheatTriticum dicoccoides, in Israel. Mol. Biol. Evol. 3: 547–558.

    CAS  Google Scholar 

  • Gebhardt, C. & F. Salamini, 1992. Restriction fragment length polymorphism analysis of plant genomes and its application to plant breeding. Intern. Rev. Cytol. 135: 201–237.

    Article  CAS  Google Scholar 

  • Gepts, P. & F. A. Bliss, 1986. Phaseolin variability among wild and cultivated common beans (Phaseolus vulgaris) in Colombia. Econ. Bot. 40: 469–478.

    CAS  Google Scholar 

  • Gepts, P. & D. G. Debouck, 1991. Origin, domestication and evolution of the common bean (P. vulgaris L.). In: A. van Schoonhoven & O. Voysest (Eds.), Common beans: research for crop improvement, pp. 7–53, Commonwealth Agricultural Bureaux International, Wallingford, United Kingdom.

    Google Scholar 

  • Gerbi, S. A., 1985. Evolution of ribosomal RNA. In: R. J. McIntyre (Ed.), Molecular evolutionary genetics, pp. 419–518, Plenum Press, New York, USA.

    Google Scholar 

  • Gerlach, W. L. & J. R. Bedbrook, 1979. Cloning and characterisation of ribosomal RNA genes from wheat and barley. Nucl. Acid. Res. 7:1869–1883.

    CAS  Google Scholar 

  • Havey, M. J., 1992. Restriction enzyme analysis of the nuclear 45S ribosomal DNA of six cultivted Alliums (Alliaceae). Pl. Syst. Evol. 181: 45–55.

    Article  CAS  Google Scholar 

  • Hemleben, V., 1990. Molekularbiologie der Pflanzen, Gustav Fischer Verlag, Stuttgart, Germany.

    Google Scholar 

  • Hemleben, V., Ganal, M., Gerstner, J., Schiebel, K. & R. A. Tones, 1988. Organisation and length heterogeneity of plant ribosomal RNA genes. In: G. Kahl (Ed.), Architecture of eucaryotic genes, pp. 371–383, VCH Verlagsgesellschaft, Weinheim, Germany.

    Google Scholar 

  • Hemleben, V., Zentgraf, U., King, K., Borisjuk, N. & G. Schweizer, 1992. Middle repetitive and highly repetitive sequences detect polymorphisms in plants. In: G. Kahl, H. Appelhans, J. Kömpf & A. J. Driesel (Eds.), DNA-polymorphisms in eucaryotic genomes, pp. 157–170, Hüthig Buch Verlag, Heidelberg, Germany.

    Google Scholar 

  • Hernandez, X., Miranda-Colin, S. & C. Prwyer, 1959. El origin dePhaseolus coccineus L.darwinianus Hernandez X. & Miranda C. subspecies nova. Rev. Soc. Mex. Hist. Nat. 20: 99–121.

    Google Scholar 

  • Jackson, D. A., Somers, K. M. & H. H. Harvey, 1989. Similarity coefficients: measures of co-occurence and association or simply measures of occurrence. Am. Nat. 133: 436–453.

    Article  Google Scholar 

  • Kaplan, L. & L. N. Kaplan, 1988.Phaseolus in archaeology. In: P. Gepts (Ed.), Genetic resources ofPhaseolus beans, pp. 125–142, Kluwer Academic Publishers, Dordrecht, The Netherlands.

    Google Scholar 

  • King, K., Torres, R. A., Zentgraf, U. & V. Hemleben, 1993. Molecular evolution of the intergenic spacer in the nuclear ribosomal RNA ofCucurbitaceae. J. Mol. Evol. 36: 144–152.

    Article  PubMed  CAS  Google Scholar 

  • Kluge, A. G. & J. S. Farris, 1969. Quantitative phyletics and evolution of anurans. Syst. Zool. 18: 1–32.

    Article  Google Scholar 

  • Maggini, F., Tucci G., Demartis, A., Gelati, M. T. & S. Avanzi, 1992. Ribosomal RNA genes ofPhaseolus coccineus. I. Plant Mol. Biol. 18: 1073–1082.

    Article  CAS  Google Scholar 

  • Maquet, A., Gutierrez, A. & D. G. Debouck, 1990. Further biochemical evidence for the existence of two gene pools in Lima beans. Ann. Rep. Bean Improv. Coop. 33: 128–129.

    Google Scholar 

  • Maréchal, R., Mascherpa, J. M. & F. Stainer, 1978. Étude taxonomique d'un groupe complexe d'espèces des genres Phaseolus etVigna (Papilionaceae) sur la base de données morphologiques et polliniques, traitées par l'analyse informatique. Boissiera 28: 1–278.

    Google Scholar 

  • Miranda-Colin, S., 1967. Infiltración genética entrePhaseolus coccineus L. Colegio de Postgraduados, Escuela National de Agricultura, Chapingo, México. Serie de Investigación 9: 1–48.

    Google Scholar 

  • Molnar, S. J., Gupta, P. K., Fedak, G. & R. Wheatcroft, 1989. Ribosomal DNA repeat unit polymorphism in 25Hordeum species. Theor. Appl. Genet. 78: 387–392.

    Article  CAS  Google Scholar 

  • Olsen, G. J. & C. R. Woese, 1993. Ribosomal RNA: a key to phylogeny? FASEB J. 7: 113–123.

    PubMed  CAS  Google Scholar 

  • Piñiero, D. & L. Eguiarte, 1988. The origin and biosystematic status ofPhaseolus coccineus ssp.polyanthus: electrophoretic evidence. Euphytica 37: 199–203.

    Google Scholar 

  • Rogers, S. O. & A. J. Bendich, 1987. Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. Plant Mol. Biol. 9: 509–520.

    Article  CAS  Google Scholar 

  • Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A. & R. W. Allard, 1984. Ribosomal spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA 81: 8014–8018.

    Article  PubMed  CAS  Google Scholar 

  • Santoni, S. & A. Berville, 1992. Characterization of the nuclear ribosomal DNA units and phylogeny ofBeta L. wild forms and cultivated beets. Theor. Appl. Genet. 83: 533–542.

    Article  Google Scholar 

  • Schaal, B. A. & G. H. Learn, 1988. Ribosomal DNA variation within and among populations. Ann. Missouri Bot. Gard. 75: 1207–1216.

    Article  Google Scholar 

  • Schmit, V. & D. G. Debouck, 1990.Phaseolus glabellus PIPER, a noteworthy variant of theP. coccineus complex? Ann. Rep. Bean Improv. Coop. 33: 124–125.

    Google Scholar 

  • Schmit, V. & D. G. Debouck, 1991. Observations on the origin ofPhaseolus polyanthus Greenman. Econ. Bot. 45: 345–364.

    Google Scholar 

  • Schmit, V., Du Jardin, P, Baudoin, J. P. & D. G. Debouck, 1992. Diversity studies of somePhaseolus taxa using chloroplast DNA as molecular marker. Ann. Rep. Bean Improv. Coop. 35: 213–214.

    Google Scholar 

  • Smith, G. E. & M. D. Summers, 1980. The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper. Anal. Biochem. 109:123–129.

    Article  PubMed  CAS  Google Scholar 

  • Sullivan, J. G. & G. Freytag, 1986. Predicting interspecific compatibilities in beans (Phaseolus) by seed protein electrophoresis. Euphytica 35: 201–209.

    Article  Google Scholar 

  • Swofford, D. L. & G. J. Olsen, 1990. Phylogeny reconstruction. In: D. M. Hillis & C. Moritz (Eds.), Molecular systematics, pp. 41–501, Sinauer Associates, Inc. Publishers, Sunderland, Massachusetts, USA.

    Google Scholar 

  • Wittmack, L., 1988. Die Heimath der Bohnen und der Kürbisse. Ber. Deutsch. Bot. Ges. 6: 374–380.

    Google Scholar 

  • Young, N. D., 1992. Restriction fragment length polymorphisms (RFLPs) and crop improvement. Exp. Agricult. 28: 385–397.

    Article  CAS  Google Scholar 

  • Zimmer, E. A., Jupe, E. R. & V. Walbot, 1988. Ribosomal gene structure, variation, and its inheritance in maize and its ancestors. Genetics 120: 1125–1136.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, and The Biotechnology Program, University of Kaiserslautern, 67653, Kaiserslautern, Germany

    Maren Jacob, Dorothea Zink & Walter Nagl

Authors
  1. Maren Jacob
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dorothea Zink
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Walter Nagl
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jacob, M., Zink, D. & Nagl, W. RFLPs of the rRNA genes in the genusPhaseolus . Genet Resour Crop Evol 42, 97–106 (1995). https://doi.org/10.1007/BF02539513

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02539513

Key words

Search

Navigation