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Predicting interspecific compatibilities in beans (Phaseolus) by seed protein electrophoresis

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Summary

Seed proteins of 17 wild species of Phaseolus were separated by electrophoresis on SDS polyacrylamide gels. There was very little variation of the protein pattern within most species, while considerable variation among species was evident. Relative interspecific similarities of protein patterns were estimated using Jaccard's similarity index, and a cluster analysis was performed on these values. The resultant dendrogram generally agreed with previous research on interspecific relationships in Phaseolus based on morphological characteristics, and also generally agrees with current information on interspecific similarities based on hybridization studies. Suggestions are made regarding interspecific hybridization among Phaseolus species which have not been attempted, but which may be possible based on cluster analysis. These hybrids include P. vulgaris × P. polystachyus, P. vulgaris × (P. polystachyus × P. metcalfei) and P. anisotrichos × P. angustissimus.

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Supported by the USDA Specific Cooperative Agreement to the University of Puerto Rico 458-7B30-0-203 ‘Bean Improvement by Interspecific Crosses’.

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Sullivan, J.G., Freytag, G. Predicting interspecific compatibilities in beans (Phaseolus) by seed protein electrophoresis. Euphytica 35, 201–209 (1986). https://doi.org/10.1007/BF00028558

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  • DOI: https://doi.org/10.1007/BF00028558

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