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Nuclear Ribosomal RNA Genes: ITS Region

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Molecular Plant Taxonomy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2222))

Abstract

Despite possible drawbacks (intraspecific polymorphisms and possible fungal contamination), sequencing of the ITS region of the ribosomal RNA genes remains one of the most popular nuclear sequences used for plant taxonomy and phylogeny. A protocol for PCR amplification and sequencing of this region using universal plant primers is provided.

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Acknowledgments

Denis Da Silva (Université de La Réunion, UMR PVBMT) and Michel Grisoni (CIRAD, UMR PVBMT) are acknowledged for their help in the ITS amplification protocol design. Adeline Trebel and Nicolas Cazanove are thanked for running ITS2 secondary structure analyses as part of their master 1 thesis work.

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Authors and Affiliations

  1. UMR PVBMT, Universite de la Reunion, St Pierre, Réunion, France

    Pascale Besse

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  1. Pascale Besse
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Correspondence to Pascale Besse .

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  1. UMR PVBMT, Universite de la Reunion, St Pierre, Réunion, France

    Pascale Besse

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Besse, P. (2021). Nuclear Ribosomal RNA Genes: ITS Region. In: Besse, P. (eds) Molecular Plant Taxonomy. Methods in Molecular Biology, vol 2222. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0997-2_7

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  • DOI: https://doi.org/10.1007/978-1-0716-0997-2_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0996-5

  • Online ISBN: 978-1-0716-0997-2

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