Skip to main content
SpringerLink
Log in

Analysis of tissue culture-derived variation in pea (Pisum sativum L.)-preliminary results

  • Published:
Euphytica Aims and scope Submit manuscript

Summary

The possibility of producing agronomically-useful somaclones via organogenesis and somatic embryogenesis from callus cultures of pea (Pisum sativum L.) was studied. Organogenic calli were induced from immature leaflets on MSB medium with NAA and BAP. Embryogenic calli were derived either from immature zygotic embryos (using 2,4-D) or from shoot apices (using picloram) of aseptically-germinated seedlings.

The seed progenies (T1 to T3-generation) of primary regenerants were grown in field conditions and their phenotypic variation was evaluated and compared with control, non-tissue culture-derived plant material. In addition, electrophoretic analyses of selected isoenzyme systems and total proteins have been done. The results do not show dramatic changes in qualitative and quantitative traits. The evaluation of at least two future generations (T4, T5) is planned.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BAP:

6-benzylaminopurine

IBA:

indole-3-butyric acid

MSB:

medium (mineral salts after Murashige & Skoog, 1962, vitamins after Gamborg et al., 1968)

NAA:

α-naphthalene-acetic acid, picloram-4-amino-3,5,6-trichloro picolinic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

ORG:

organogenesis

SE:

somatic embryogenesis

References

  • Bajaj Y.P.S. (Ed), 1990. Biotechnology in Agriculture and Forestry H, Somaclonal Variation in Crop Improvement I. Springer-Verlag, Berlin-Heidelberg-New York.

    Google Scholar 

  • Cecchini E., L. Natali, A. Cavallini & M. Durante, 1992. DNA variations in regenerated plants of pea (Pisum sativum L.). Theor. Appl. Genet. 84: 874–879.

    Article  CAS  Google Scholar 

  • De Klerk G.-J., 1990. How to measure somaclonal variation. Acta Bot. Neerl. 39: 129–144.

    Google Scholar 

  • Gamborg O.L., R.A. Miller & K. Ojima, 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158.

    Article  PubMed  CAS  Google Scholar 

  • Griga, M., 1990. The study of regeneration systems in vitro in pea, faba bean and soybean. Ph.D.-Thesis, Masaryk University, Bmo 1990 (in Czech).

  • Griga, M., J. Stejskal & H. Klenotičová, 1992. Plant regeneration in soybean, pea and faba bean via somatic embryogenesis. p. 107–108. In: Proc. 1st Europ. Conf. on Grain Legumes. Angers, France.

  • Griga M., E. Tejklová, F.J. Novák & M. Kubaláková, 1986. In vitro clonal propagation of Pisum sativum L. Plant Cell Tissue Organ Cult. 6: 95–104.

    Article  CAS  Google Scholar 

  • Hussey G. & H.V. Gunn, 1984. Plant production in pea (Pisum sativum L. cvs. Puget and Upton) from long-term callus with superficial meristems. Plant Sci. Lett. 37: 143–148.

    Article  CAS  Google Scholar 

  • Kysely W., J.R. Myers, P.A. Lazzeri, G.B. Collins & H.J. Jacobsen, 1987. Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.). Plant Cell Rep. 6: 305–308.

    Article  CAS  Google Scholar 

  • Kysely W. & H.J. Jacobsen, 1990. Somatic embryogenesis from pea embryos and shoot apices. Plant Cell Tissue Organ Cult. 20: 7–14.

    Article  CAS  Google Scholar 

  • Lehminger-Mertens R. & H.J. Jacobsen, 1989. Plant regeneration from pea protoplasts via somatic embryogenesis. Plant Cell Rep. 8: 379–382.

    Article  Google Scholar 

  • Lutova L.A. & E.K. Zabelina, 1988. Callus and shoot formation in different forms of Pisum sativum L. in vitro. Genetika 24: 1632–1640 (in Russian).

    Google Scholar 

  • Mikhailova-Krumova A.B., I.P. Gavrilyuk, M.I. Azarkovich & R.G. Butenko, 1991. Study of somaclonal variations in pea seed proteins. Fiziol. Rast. 38: 521–529 (in Russian).

    CAS  Google Scholar 

  • Mroginski L.A. & K.K. Kartha, 1981. Regeneration of pea (Pisum sativum L. cv. Century) plants by in vitro culture of immature leaflets. Plant Cell Rep. 1: 64–66.

    Article  Google Scholar 

  • Murashige T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 472–497.

    Article  Google Scholar 

  • Natali L. & A. Cavallini, 1987. Nuclear cytology of callus and plantlets regenerated from pea (Pisum sativum L.) meristems. Protoplasma 141: 121–125.

    Article  Google Scholar 

  • Özcan S., M. Barghchi, S. Firek & J. Draper, 1993. Efficient adventitious shoot regeneration and somatic embryogenesis in pea. Plant Cell Tissue Organ Cult. 34: 271–277.

    Article  Google Scholar 

  • Rubluo A., K.K. Kartha, L.A. Mroginski & J. Dyck, 1984. Plant regeneration from pea leaflets cultured in vitro and genetic stability of regenerants. J. Plant Physiol. 117: 119–130.

    CAS  Google Scholar 

  • Stejskal J. & M. Griga, 1992. Somatic embryogenesis and plant regeneration in Pisum sativum L. Biol. Plant 34: 15–22.

    Article  Google Scholar 

  • Tétu T., R.S. Sangwan & B.S. Sangwan-Norreel, 1990. Direct somatic embryogenesis and organogenesis in cultured immature zygotic embryos of Pisum sativum L. J. Plant Physiol. 137: 102–109.

    Google Scholar 

  • Van Doorne L.E., G. Marshall & R.C. Kirkwood, 1991. Development of herbicide tolerance in peas. II. Regeneration via somatic embryogenesis. p. 271–274. In: R.J. Froud-Williams, P. Gladders, M.C. Heath, J.F. Jenkyn, C.M. Knott, A. Lane & D. Pink (Eds). Aspects of Appl. Biol. 27, Production and Protection of Legumes. Assoc. Appl. Biol., Wellesbourne, Warwick.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. OSEVA, Research Institute of Technical Crops & Legumes, 787 01, Šumperk, Czech Republic

    Miroslav Griga & Jaromír Stejskal

  2. Research Institute for Cattle Breeding, Rapotín 267, 787 13, Vikýřovice, Czech Republic

    Karel Beber

Authors
  1. Miroslav Griga
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jaromír Stejskal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Karel Beber
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Griga, M., Stejskal, J. & Beber, K. Analysis of tissue culture-derived variation in pea (Pisum sativum L.)-preliminary results. Euphytica 85, 335–339 (1995). https://doi.org/10.1007/BF00023964

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00023964

Key words

Search

Navigation