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Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.)

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Abstract

Whole plant regeneration via somatic embryogenesis was obtained in pea (Pisum sativum L.) using explants from immature embryos or shoot apex segments. The induction of somatic embryos required picloram or 2,4-D. Germination of fully-developed embryos was accomplished by subculture on medium with only cytokinin and then on medium supplemented with cytokinins in combination with a reduced auxin concentration. Plantlets obtained from both zygotic embryos and shoot apices were transferred to soil and were grown to maturity. Nine plants were examined cytologically, revealing three tetraploids (2n=4x=28) and six diploids (2n=2x=14).

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Abbreviations

Picloram:

4-amino-3,5,6-trichloropicolinic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

1-naphthaleneacetic acid

BA:

6-benzylaminopurine

IBA:

indole-3-butyric acid

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Author notes

  1. Wilfried Kysely

    Present address: Institut für Genetik, Universität Bonn, Kirschallee 1, D-5300, Bonn 1, Germany

  2. James R. Myers

    Present address: Research and Extension Center, University of Idaho, 83341, Kimberly, ID, USA

  3. Paul A. Lazzeri

    Present address: Max-Planck-Institut, D-5000, Köln 30, Germany

Authors and Affiliations

  1. Department of Agronomy, University of Kentucky, 40546-0091, Lexington, KY, USA

    Wilfried Kysely, James R. Myers, Paul A. Lazzeri & Glenn B. Collins

  2. Institut für Genetik, Universität Bonn, Kirschallee 1, D-5300, Bonn 1, Germany

    Hans-Jorg Jacobsen

Authors
  1. Wilfried Kysely
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  2. James R. Myers
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  3. Paul A. Lazzeri
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  4. Glenn B. Collins
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  5. Hans-Jorg Jacobsen
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Additional information

Communicated by O.L. Gamborg

KAES Journal Article No. 87-3-4

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Kysely, W., Myers, J.R., Lazzeri, P.A. et al. Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.). Plant Cell Reports 6, 305–308 (1987). https://doi.org/10.1007/BF00272005

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  • DOI: https://doi.org/10.1007/BF00272005

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