Abstract
In vitro regeneration of plants from immature leaflets of 3 day-old pea (Pisum sativum L. cv. Century) seedlings was studied under defined nutritional, hormonal and environmental conditions. Immature leaflets isolated from the second and third apical leaves of aseptically germinated seeds were cultured on MS medium containing vitamins as in B5 medium, 3% sucrose, 0.8% agar and supplemented with 0.1, 1, and 10 μM concentrations of naphthaleneacetic acid (NAA) and 1 and 10 μM levels of benzyladenine (BA) in various combinations. Shoot regeneration from the primary callus occurred within 45 to 90 days of culture in most of the hormone combinations. Although the number of calli producing shoots was maximal at 10 μM levels of NAA and BA, multiple shoot regeneration was predominant at a combination of 0.1 μM NAA and 10 μM BA. Indoleacetic acid (IAA) and kinetin (K), both at 10 μM, also induced shoot regeneration. No shoots were regenerated when 10 day-old leaflets were used as explants. Root production generally occurred on non-shoot regenerating calli. Roots were induced to differentiate by transferring the regenerated shoots onto half-strength B5 medium supplemented with 1 μM NAA.
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Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50: 151–158
Gamborg OL, Constabel F, Shyluk JP (1974) Physiol. Plant. 30: 125–128
Haskins RH, Kartha KK (1980) Can. J. Bot. 58: 833–840
Jacobsen HJ, Ingensiep HW, Herlt M, Kaul MLH (1980) In: Sala F, Parisi B, Cella R, Ciferri O (eds.) Plant Cell Cultures: Results and Perspectives, Elsevier/North Holland Biomedical Press. pp 319–324
Kameya T, Widholm J (1981) Plant Sci. Lett. 21: 289–294
Kartha KK, Gamborg OL, Constabel F (1974) Z. Pflanzenphysiol. 72: 172–176
Kartha KK, Gamborg OL (1979) In: Maraite H, Meyer JA (eds.) Diseases of tropical food crops, Universite Catholique de Louvain, Belgium, pp 267–283
Kartha KK, Leung NL, Gamborg OL (1979) Plant Sci. Lett. 15: 7–15
Malmberg RL (1979) Planta 146: 243–244
McComb AJ (1977) In: Sutcliffe JF, Pate JS (eds.) The physiology of the garden pea, Academic Press, London, pp 235–263
Mroginski LA, Kartha KK Shyluk JP (1981) Can. J. Bot. 59: 826–830
Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497
Thomas E, Wernicke W (1978) In: Thorpe TA (ed.) Frontiers of Plant Tissue Culture 1978. University of Calgary, Canada, pp 403–410
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NRCC # 19712
Visiting Scientist, supported by a research fellowship from “Consejo Nacional de Investigaciones Científicas y Técnicas (Rep. Argentina)”. Permanent address: Facultad de Ciencias Agrarias, IBONE, Casilla de Correos 209, Corrientes (3400), Argentina.
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Mroginski, L.A., Kartha, K.K. Regeneration of pea (Pisum sativum L. cv. Century) plants by in vitro culture of immature leaflets. Plant Cell Reports 1, 64–66 (1981). https://doi.org/10.1007/BF00269274
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DOI: https://doi.org/10.1007/BF00269274