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Somatic embryogenesis and plant regeneration inPisum sativum L.

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Biologia Plantarum

Abstract

Somatic embryogenesis was induced in immature zygotic embryos of pea (Pisum sativum L.), synthetic auxins α-naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-amino-3,5,6-trichloropicolinic acid (picloram, PIC) being used. Only one (line HM-6) of 46 genotypes tested exhibited good potential for somatic embryogenesis. 2,4-D was found as the best somatic embryo inductor. Three different ways of somatic embryo conversion have been described. Plantlets from individual somatic embryos were micropropagated as somaclones and subsequently rooted. A sterile morphological mutant has been found within a group of fertile plants of T0-generation. Sufficient amount of T1-seeds is available for somaclonal variation studies.

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Authors and Affiliations

  1. Oseva Research and Breeding Institute of Technical Crops and Legumes, CS -78701, Šumperk, Czechoslovakia

    J. Stejskal & M. Griga

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  1. J. Stejskal
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  2. M. Griga
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Stejskal, J., Griga, M. Somatic embryogenesis and plant regeneration inPisum sativum L.. Biol Plant 34, 15–22 (1992). https://doi.org/10.1007/BF02925784

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  • DOI: https://doi.org/10.1007/BF02925784

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