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Involvement of CsCDPK20 and CsCDPK26 in Regulation of Thermotolerance in Tea Plant (Camellia sinensis)

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Abstract

Calcium-dependent protein kinases (CDPKs) play critical roles in the regulation of plant growth, development, and stress response. In this study, Camellia sinensis CsCDPK1, CsCDPK3, CsCDPK20, and CsCDPK26 were cloned and functionally characterized. Bioinformatics analyses showed that all analyzed CsCDPK genes encoded the expected CDPK structures. Subcellular localization indicated that the four CsCDPK proteins were localized to the cytoplasm and nucleus in onion epidermal cells. The quantitative real-time PCR results indicated that the four CsCDPK genes exhibited tissue-specific expression patterns. Exposure to heat stress and exogenous abscisic acid induced increases in CsCDPK20 and CsCDPK26 transcript abundance at different time points. Furthermore, overexpression of CsCDPK20 and CsCDPK26 increased the thermotolerance in transgenic Arabidopsis thaliana plants. The proline content in leaves was significantly higher in transgenic plants than that in wild-type under heat stress, whereas malondialdehyde content was lower in transgenic plants. Additionally, expression of stress-responsive genes (i.e., AtAPX1, AtPOD, AtProT1, AtP5CS2, AtHSFA2, AtHSP70, AtHSP101, AtRD29B, AtRAB18, AtABI1, AtRBOHD, and AtRBOHF) was increased in the CsCDPK20 and CsCDPK26 transgenic A. thaliana plants compared with that in the wild type. Collectively, our results suggest that CsCDPK20 and CsCDPK26 may act as positive regulator in C. sinensis response to heat stress.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (31470690), the earmarked fund for Modern Agro-industry Technology Research System (CARS-19), and the Fundamental Research Funds for the Central Universities (2662017QD036).

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Authors and Affiliations

  1. Tea Research Institute, Nanjing Agricultural University, Weigang No. 1, Nanjing, 210095, Jiangsu Province, People’s Republic of China

    Mingle Wang, Qinghui Li, Kang Sun, Xuan Chen, Qiongqiong Zhou, Huan Li & Xinghui Li

  2. College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China

    Mingle Wang & Xuyang Zhang

  3. State Key Laboratory of Agricultural Microbiology, Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, China

    Qinghui Li

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  1. Mingle Wang
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Contributions

MLW performed the experiments and drafted the manuscript. QHL helped in data collection, sample preparation, and RNA extraction. KS, XYZ, QQZ, and HL revised the language of the manuscript. XC contributed with valuable discussions. XHL managed and designed the research and experiments.

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Correspondence to Xinghui Li.

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Wang, M., Li, Q., Sun, K. et al. Involvement of CsCDPK20 and CsCDPK26 in Regulation of Thermotolerance in Tea Plant (Camellia sinensis). Plant Mol Biol Rep 36, 176–187 (2018). https://doi.org/10.1007/s11105-018-1068-0

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