Abstract
The main theme of the present study was to investigate whether the bovine breast cancer 1 (BRCA1) gene was associated with mastitis resistance in Frieswal (HF × Sahiwal) cattle. Two SNPs (G22231T, T25025A) were genotyped by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and 4 combinations (haplotypes) of these SNPs were observed. The single SNP and their genetic effects on somatic cell score (SCS) were evaluated and a significant association with SCS was found for both the SNP. The mean SCS of individuals with genotype AA for G22231T and CD for T25025A were significantly (P < 0.01) lower than those of genotypes AB and DD respectively. The results of combined genotypes analysis of two SNPs showed that ABDD and AACD haplotypes with significantly (P < 0.05) highest and lowest SCS.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Deb R, Kumar A, Chakraborty S, Verma AK, Tiwari R, Dhama K, Singh U, Kumar S (2013) Trends in diagnosis and control of bovine mastitis. Pak J Biol Sci 16:1653–1661
Deb R, Singh U, Kumar S, Kumar A, Sharma A, Mann S, Singh R (2013) TIR domain of bovine TLR4 gene in Frieswal crossbred cattle: an early marker for mastitis resistance. Indian J Anim Sci 83:66–68
Zhang LP, Gan QF, Ma TH, Li HD, Wang XP, Li JY, Gao X, Chen JB, Ren HY, Xu SZ (2009) Toll-like receptor 2 gene polymorphism and its relationship with SCS in dairy cattle. Anim Biotechnol 20:87–95
Deb R, Singh U, Kumar S, Mann S, Singh R, Sengar G, Kumar A, Sharma A (2013) Genotypic to expression profiling of bovine calcium channel, voltage-dependent, alpha-2/delta subunit 1 gene and their association with bovine mastitis among Frieswal (HF × Sahiwal) crossbred cattle of Indian origin. Anim Biotechnol. doi:10.1080/10495398.2013.836106
Yuan ZR, Li J, Liu L, Zhang LP et al (2011) Single nucleotide polymorphism of CACNA2D1 gene and its association with milk somatic cell score in cattle. Mol Biol Rep 38:5179–5183
Chu MX, Ye SC, Qiao L, Wang JX, Feng T, Huang DW, Cao GL, Di R, Fang L, Chen GH (2012) Polymorphism of exon 2 of BoLA-DRB3 gene and its relationship with somatic cell score in Beijing Holstein cows. Mol Biol Rep 39:2909–2914
Youngerman SM, Saxton AM, Oliver SP, Pighetti GM (2004) Association of CXCR2 polymorphisms with subclinical and clinical mastitis in dairy cattle. J Dairy Sci 87:2442–2448
Mahfoudh W, Bouaouina N, Ahmed SB, Gabbouj S, Shan J, Mathew R, Uhrhammer N, Bignon YJ, Troudi W, Elgaaied AB, Hassen E, Chouchane L (2012) Hereditary breast cancer in Middle Eastern and North African (MENA) populations: identification of novel, recurrent and founder BRCA1 mutations in the Tunisian population. Mol Biol Rep 39:1037–1046
Xu J, Wang B, Zhang Y, Li R, Wang Y, Zhang S (2011) Clinical implications for BRCA gene mutation in breast cancer. Mol Biol Rep. doi:10.1007/s11033-011-1073-y
Bennewitz J, Reinsch N, Guiard V, Fritz S, Thomsen H, Looft C, Kuhn C, Schwerin M, Weimann C, Erhardt G, Reinhardt F, Reents R, Boichard D, Kalm E (2004) Multiple quantitative trait loci mapping with cofactors and application of alternative variants of the false discovery rate in an enlarged granddaughter design. Genetics 168:1019–1027
Krum SA, Womack JE, Lane TF (2003) Bovine BRCA1 shows classic responses to genotoxic stress but low in vitro transcriptional activation activity. Oncogene 22:6032–6044
Yuan Z, Chu G, Dan Y, Li J, Zhang L, Gao X, Gao H, Li J, Xu S, Liu Z (2012) BRCA1: a new candidate gene for bovine mastitis and its association analysis between single nucleotide polymorphisms and milk somatic cell score. Mol Biol Rep 39(6):6625–6631. doi:10.1007/s11033-012-1467-5
Schalm OW, Carroll EJ, Jain NC (1971) Bovine Mastitis. Lea & Febiger, Philadelphia
Philipsson J, Ral G, Berglund B (1995) Somatic-cell count as a selection criterion for mastitis resistance in dairy-cattle. Livest Prod Sci 41:195–200
Shook GE, Schutz MM (1994) Selection on somatic cell score to improve resistance to mastitis in the United States. J Dairy Sci 77:648–658
Chen R, Yang Z, Ji D, Mao Y, Chen Y, Li Y, Wu H, Wang X, Chang L (2011) Polymorphisms of the IL8 gene correlate with milking traits, SCS and mRNA level in Chinese Holstein. Mol Biol Rep 38:4083–4088
Daetwyler HD, Schenkel FS, Sargolzaei M, Robinson JA (2008) A genome scan to detect quantitative trait loci for economically important traits in Holstein cattle using two methods and a dense single nucleotide polymorphism map. J Dairy Sci 91:3225–3236
Singh U, Kumar S, Deb R, Mann S, Sharma A (2013) Genotypic profiling of coding region of leptin gene and their association studies on reproductive and milk production traits in Sahiwal and Frieswal cattle of India. Afr J Biotechnol 12(42):6140–6146. doi:10.5897/AJB2013.13061
Zheng X, Ju Z, Wang J et al (2011) Single nucleotide polymorphisms, haplotypes and combined genotypes of LAP3 gene in bovine and their association with milk production traits. Mol Biol Rep 38:4053–4061
Fallin D, Cohen A, Essioux L et al (2001) Genetic analysis of case/control data using estimated haplotype frequencies: application to APOE locus variation and Alzheimer’s disease. Genome Res 11:143–151
Acknowledgments
Authors are thankful to the Director, CIRC, ICAR, Meerut, UP, India for providing necessary facilities to carry out the present work. Authors also express their gratitude to the Military Farm, Meerut, UP, India for providing necessary help for the present study.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Deb, R., Singh, U., Kumar, S. et al. Profiling of Bovine Breast Cancer 1, Early Onset (BRCA1) Gene Among Frieswal (HF × Sahiwal) Cows and Their Association with Mastitis. Natl. Acad. Sci. Lett. 37, 579–583 (2014). https://doi.org/10.1007/s40009-014-0283-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40009-014-0283-y