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Case–control approach application for finding a relationship between candidate genes and clinical mastitis in Holstein dairy cattle

  • Animal Genetics • Original Paper
  • Published:
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Abstract

Mastitis is a major source of economic loss in dairy herds. The objective of this research was to evaluate the association between genotypes within SLC11A1 and CXCR1 candidate genes and clinical mastitis in Holstein dairy cattle using the selective genotyping method. The data set contained clinical mastitis records of 3,823 Holstein cows from two Holstein dairy herds located in two different regions in Iran. Data included the number of cases of clinical mastitis per lactation. Selective genotyping was based on extreme values for clinical mastitis residuals (CMR) from mixed model analyses. Two extreme groups consisting of 135 cows were formed (as cases and controls), and genotyped for the two candidate genes, namely, SLC11A1 and CXCR1, using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), respectively. Associations between single nucleotide polymorphism (SNP) genotypes with CMR and breeding values for milk and protein yield were carried out by applying logistic regression analyses, i.e. estimating the probability of the heterogeneous genotype in the dependency of values for CMR and breeding values (BVs). The sequencing results revealed a novel mutation in 1139 bp of exon 11 of the SLC11A1 gene and this SNP had a significant association with CMR (P < 0.05). PCR-RFLP analysis leads to three banding patterns for CXCR1c.735C>G and these genotypes had significant relationships with CMR. Overall, the results showed that SLC11A1 and CXCR1 are valuable candidate genes for the improvement of mastitis resistance as well as production traits in dairy cattle populations.

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Acknowledgements

Data and blood samples were kindly obtained from two dairy cattle farms (Foka and Taliseh). The authors are thankful for the help of these farms’ owners and staff.

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Authors and Affiliations

  1. Department of Animal Science, Tehran University, Daneshkade Avenue, Pardis of Agriculture and Natural Resources, Karaj, Iran

    Masoumeh Bagheri, M. Moradi-Sharhrbabak, R. Miraie-Ashtiani & M. Safdari-Shahroudi

  2. Department of Animal Breeding, Kassel University, Kassel, Germany

    Masoumeh Bagheri

  3. Department of Animal and Poultry Science, Abureyhan Campus, University of Tehran, Pakdasht, Iran

    R. Abdollahi-Arpanahi

Authors
  1. Masoumeh Bagheri
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  2. M. Moradi-Sharhrbabak
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  3. R. Miraie-Ashtiani
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  4. M. Safdari-Shahroudi
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  5. R. Abdollahi-Arpanahi
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Correspondence to Masoumeh Bagheri.

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Communicated by: Maciej Szydlowski

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Bagheri, M., Moradi-Sharhrbabak, M., Miraie-Ashtiani, R. et al. Case–control approach application for finding a relationship between candidate genes and clinical mastitis in Holstein dairy cattle. J Appl Genetics 57, 107–112 (2016). https://doi.org/10.1007/s13353-015-0299-0

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  • DOI: https://doi.org/10.1007/s13353-015-0299-0

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