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Polymorphisms of the IL8 gene correlate with milking traits, SCS and mRNA level in Chinese Holstein

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Abstract

To explore the relation of Interleukin-8 (IL8) gene polymorphism with immunity against mastitis in dairy cow, the polymorphism of IL8 gene was investigated in 610 Chinese Holstein cow from 30 bull families in a dairy farm in Shanghai using polymerase chain reaction–single strand conformation polymorphism (PCR–SSCP) technique, and milk yield, milk fat percentage, milk protein percentage, 305 day corrected milk yield, 305 day milk fat yield, 305 day milk protein yield and somatic cell score (SCS) were measured and analyzed, and the mRNA levels of IL8 genotypes in blood were detected by real-time PCR. The results showed that three genotypes, KK, KA and AA were detected with frequencies of 0.187, 0.451, and 0.362, respectively. The gene frequencies of K and A were 0.412 and 0.588, respectively. The significant association of the IL8 mutations with milk yield, 305 day milk protein yield, 305 day corrected milk yield and 305 day milk fat yield, SCS, and significant association with milk protein percentage were identified, while their association with milk fat percentage were not significant. KK had higher milk yield, 305 day milk protein yield, 305 day corrected milk yield and 305 day milk fat yield than AA or KA, and the least square mean of SCS of KK was significantly lower than that of AA or KA. AA had significant lower milk protein yield than KK or KA. The relative IL8 mRNA level of KK in blood was the highest. These findings demonstrated that IL8 genotype significantly correlated with mastitis resistance and the locus could be a useful genetic marker for mastitis resistance selection and breeding in Chinese Holstein.

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Acknowledgments

The research was financially supported by the National “863” Program of China (2008AA10Z144), the Natural Science Foundation Key Research of University of Jiangsu Province (09KJA230002), and the Technology Supported Program of Jiangsu Province (BE2008302-2),the Natural Science Foundation Research of University of Jiangsu Province (08KJB230005).

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  1. Animal Science and Technology College, Yangzhou University, Yangzhou, Jiangsu, China

    Renjin Chen, Zhangping Yang, Dejun Ji, Yongjiang Mao, Ying Chen, Yunlong Li, Haitao Wu, Xiaolong Wang & Lingling Chang

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  1. Renjin Chen
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  2. Zhangping Yang
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  3. Dejun Ji
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  4. Yongjiang Mao
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  7. Haitao Wu
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  8. Xiaolong Wang
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  9. Lingling Chang
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Correspondence to Zhangping Yang.

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Chen, R., Yang, Z., Ji, D. et al. Polymorphisms of the IL8 gene correlate with milking traits, SCS and mRNA level in Chinese Holstein. Mol Biol Rep 38, 4083–4088 (2011). https://doi.org/10.1007/s11033-010-0528-x

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  • DOI: https://doi.org/10.1007/s11033-010-0528-x

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