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Locating QTLs controlling overwintering seedling rate in perennial glutinous rice 89-1 (Oryza sativa L.)

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Abstract

A new cold tolerant germplasm resource named glutinous rice 89-1 (Gr89-1, Oryza sativa L.) can overwinter using axillary buds, with these buds being ratooned the following year. The overwintering seedling rate (OSR) is an important factor for evaluating cold tolerance. Many quantitative trait loci (QTLs) controlling cold tolerance at different growth stages in rice have been identified, with some of these QTLs being successfully cloned. However, no QTLs conferring to the OSR trait have been located in the perennial O. sativa L. To identify QTLs associated with OSR and to evaluate cold tolerance. 286 F12 recombinant inbred lines (RILs) derived from a cross between the cold tolerant variety Gr89-1 and cold sensitive variety Shuhui527 (SH527) were used. A total of 198 polymorphic simple sequence repeat (SSR) markers that were distributed uniformly on 12 chromosomes were used to construct the linkage map. The gene ontology (GO) annotation of the major QTL was performed through the rice genome annotation project system. Three main-effect QTLs (qOSR2, qOSR3, and qOSR8) were detected and mapped on chromosomes 2, 3, and 8, respectively. These QTLs were located in the interval of RM14208 (35,160,202 base pairs (bp))–RM208 (35,520,147 bp), RM218 (8,375,236 bp)–RM232 (9,755,778 bp), and RM5891 (24,626,930 bp)–RM23608 (25,355,519 bp), and explained 19.6%, 9.3%, and 11.8% of the phenotypic variations, respectively. The qOSR2 QTL displayed the largest effect, with a logarithm of odds score (LOD) of 5.5. A total of 47 candidate genes on the qOSR2 locus were associated with 219 GO terms. Among these candidate genes, 11 were related to cell membrane, 7 were associated with cold stress, and 3 were involved in response to stress and biotic stimulus. OsPIP1;3 was the only one candidate gene related to stress, biotic stimulus, cold stress, and encoding a cell membrane protein. After QTL mapping, a total of three main-effect QTLs—qOSR2, qOSR3, and qOSR8—were detected on chromosomes 2, 3, and 8, respectively. Among these, qOSR2 explained the highest phenotypic variance. All the QTLs elite traits come from the cold resistance parent Gr89-1. OsPIP1;3 might be a candidate gene of qOSR2.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (31670326), the key Natural Science Foundation of Chongqing (CSTC2015jcyjBX0091), the Program for Innovative Research Team in University, Chongqing (CXTDX201601018).

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  1. Xiaoshu Deng and Lu Gan had made the equal contribution for the research.

Authors and Affiliations

  1. Chongqing Normal University/Chongqing Engineering Research Center of Specialty Crop Resources, Chongqing, 401331, China

    Xiaoshu Deng, Lu Gan, Yan Liu, Ancai Luo, Jiao Chen, Ruyu Tang, Lixia Lei, Jianghong Tang, Jiani Zhang & Zhengwu Zhao

  2. Chongqing University, Chongqing, 401331, China

    Liang Jin

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  1. Xiaoshu Deng
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  2. Lu Gan
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  3. Yan Liu
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  11. Zhengwu Zhao
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Correspondence to Zhengwu Zhao.

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Deng, X., Gan, L., Liu, Y. et al. Locating QTLs controlling overwintering seedling rate in perennial glutinous rice 89-1 (Oryza sativa L.). Genes Genom 40, 1351–1361 (2018). https://doi.org/10.1007/s13258-018-0731-z

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  • DOI: https://doi.org/10.1007/s13258-018-0731-z

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