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The rice OsLpa1 gene encodes a novel protein involved in phytic acid metabolism

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Abstract

The rice low phytic acid 1 (lpa1) mutant was originally identified using a forward genetics approach. This mutant exhibits a 45% reduction in rice seed phytic acid with a molar-equivalent increase in inorganic phosphorus; however, it does not appear to differ significantly in productivity from its wild-type progenitor. A second lpa1 mutant was identified from additional screening for high seed inorganic phosphorus phenotypes. Using a positional cloning strategy, we identified a single candidate gene at the rice Lpa1 locus. Sequence analysis of the candidate gene from the lpa1 mutants revealed two independent mutations (a single base pair substitution and a single base pair deletion) that confirmed the identification of this candidate as the rice low phytic acid 1 gene, OsLpa1. The OsLpa1 gene has three splice variants. The location and nature of the two mutations suggests that these lesions only affect the translation of the predicted protein derived from the longest transcript. The proteins encoded by OsLpa1 do not have homology to any of the inositol phosphate metabolism genes recently characterized in plants, although there is homology to 2-phosphoglycerate kinase, an enzyme found in hyperthermophilic methanogens that catalyzes the formation of 2,3-bisphosphoglycerate from 2-phosphoglycerate. OsLpa1 represents a novel gene involved in phytic acid metabolism.

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Acknowledgments

This was supported by National Research Initiative Competitive Grant 2005-35301-15708 from the USDA Cooperative State Research, Education, and Extension Service (T.H.T). We gratefully acknowledge technical assistance from P.M. Colowit and D. Farzaneh. We are also thankful to J.N. Rutger and V. Raboy for critical reading of the manuscript and helpful suggestions for improvement.

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Authors and Affiliations

  1. USDA-ARS Crops Pathology and Genetics Research Unit, Department of Plant Sciences, University of California at Davis, One Shields Avenue, Davis, CA, 95616, USA

    S. I. Kim & T. H. Tai

  2. Department of Plant Sciences, University of California at Davis, One Shields Avenue, Davis, CA, 95616, USA

    C. B. Andaya & S. S. Goyal

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  1. S. I. Kim
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  2. C. B. Andaya
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  3. S. S. Goyal
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  4. T. H. Tai
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Correspondence to T. H. Tai.

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Communicated by Y. Xu.

S.I. Kim and C.B. Andaya contributed equally to this work. The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.

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Kim, S.I., Andaya, C.B., Goyal, S.S. et al. The rice OsLpa1 gene encodes a novel protein involved in phytic acid metabolism. Theor Appl Genet 117, 769–779 (2008). https://doi.org/10.1007/s00122-008-0818-z

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  • DOI: https://doi.org/10.1007/s00122-008-0818-z

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