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Mapping of quantitative trait loci controlling low-temperature germinability in rice (Oryza sativa L.)

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Abstract

Low-temperature germination is one of the major determinants for stable stand establishment in the direct seeding method in temperate regions, and at high altitudes of tropical regions. Quantitative trait loci (QTLs) controlling low-temperature germinability in rice were identified using 122 backcross inbred lines (BILs) derived from a cross between temperate japonica varieties, Italica Livorno and Hayamasari. The germination rate at 15°C was measured to represent low-temperature germination and used for QTL analysis. The germination rate at 15°C for 7 days of Italica Livorno and Hayamasari was 98.7 and 26.8%, respectively, and that of BILs ranged from 0 to 83.3%. Using restriction fragment length polymorphism (RFLP) and simple sequence repeat (SSR) markers, we constructed a linkage map which corresponded to about 90% of the rice genome. Three putative QTLs associated with low-temperature germination were detected. The most effective QTL, qLTG-3-1 on chromosome 3, accounted for 35.0% of the total phenotypic variation for low-temperature germinability. Two additional QTLs, qLTG-3-2 on chromosome 3 and qLTG-4 on chromosome 4, were detected and accounted for 17.4 and 5.5% of the total phenotypic variation, respectively. The Italica Livorno alleles in all detected QTLs increased the low-temperature germination rate.

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Acknowledgments

This work was supported in part by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project DM-1102).

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Author notes

  1. T. Sato

    Present address: Hokkaido Prefectural Kamikawa Agriculture Experimental Station, Pippu, 078-0397, Hokkaido, Japan

  2. S. Y. Lin

    Present address: Honda R & D Co., Ltd., Creationcore Kazusa, 1688 Kamisawata, Yana, Kisarazu, 292-0812, Chiba, Japan

  3. Y. Takeuchi

    Present address: National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, 3058518, Ibaraki, Japan

Authors and Affiliations

  1. Hokkaido Green-Bio Institute, Naganuma, 067-1317, Hokkaido, Japan

    K. Fujino, H. Sekiguchi & T. Sato

  2. Hokkaido Prefectural Kamikawa Agriculture Experimental Station, Pippu, 078-0397, Hokkaido, Japan

    H. Kiuchi

  3. Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, 305-0854, Ibaraki, Japan

    Y. Nonoue, Y. Takeuchi, T. Ando & S. Y. Lin

  4. Department of Molecular Genetics, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602, Ibaraki, Japan

    M. Yano

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  1. K. Fujino
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  2. H. Sekiguchi
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  8. S. Y. Lin
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  9. M. Yano
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Corresponding author

Correspondence to K. Fujino.

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Communicated by F. Salamini

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Fujino, K., Sekiguchi, H., Sato, T. et al. Mapping of quantitative trait loci controlling low-temperature germinability in rice (Oryza sativa L.). Theor Appl Genet 108, 794–799 (2004). https://doi.org/10.1007/s00122-003-1509-4

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  • DOI: https://doi.org/10.1007/s00122-003-1509-4

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