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Somatic embryogenesis from bud and leaf explants of date palm (Phoenix dactylifera L.) cv. Najda

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Abstract

An efficient regeneration system through somatic embryogenesis was developed for date palm cv. Najda. Adventitious bud and proximal leaf segments cultured on Murashige and Skoog (MS) medium supplemented with various combinations of auxins and cytokinins induced embryogenesis after at least 6 months of culture. Somatic embryogenesis induction seemed correlated with the type of the explant, the induction period and the auxin used. The highest rate of somatic embryogenesis (86.0%) was obtained on bud explants cultured on MS medium supplemented with 45.0 µM 2,4-dichlorophenoxyacetic acid (2,4-D), and 4.5 µM kinetin or 4.5 µM 6-(dimethylallylamino) purine (2iP). Whereas, low levels of embryogenesis were obtained on media supplemented with 1-naphthalene acetic acid (NAA) or 2-naphthoxyacetic acid (NOA). Proximal leaf segments showed somatic embryogenesis only when cultured on media supplemented with 2,4-D or picloram. Statistical analysis revealed significant effects of explant type and plant growth regulators (PGRs) combination on somatic embryogenesis. Somatic embryos were germinated successfully on PGR-free MS medium with or without activated charcoal (50.0–60.0 and 26.6–36.6%, respectively), and 80.0% of plantlets survived after transferring to a glasshouse for 6 months. Our results will be useful for large-scale propagation of date palm cv. Najda, characterized by high fruit quality and bayoud disease resistance.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

2iP:

6-(Dimethylallylamino) purine

AC:

Activated charcoal

FW:

Fresh weight

MS:

Murashige and Skoog

NAA:

1-Naphthalene acetic acid

NOA:

2-Naphthoxyacetic acid

PGR:

Plant growth regulator

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Acknowledgements

Part of this research was funded by the Centre National pour la Recherche Scientifique et Technique (CNRST, Morocco) through the PPR2 program.

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Authors and Affiliations

  1. Institut National de la Recherche Agronomique, CRRA-Marrakech, UR Agro-Biotechnologie, Laboratoire de Biotechnologie Végétale, Avenue Mohammed 6, BP 533, Marrakech, Morocco

    Mouaad Amine Mazri

  2. Ecole Nationale d’Agriculture, Laboratoire de Culture In Vitro, Département des Sciences de Base, BP S/40, Meknes, Morocco

    Ilham Belkoura & Boutaïna Mokhless

  3. Institut National de la Recherche Agronomique, CRRA-Errachidia, UR Systèmes Oasiens, Laboratoire National de Culture des Tissus du Palmier Dattier, Avenue Moulay Ali Cherif, B.P. 2, Errachidia, Morocco

    Reda Meziani

  4. Institut National de la Recherche Agronomique, CRRA-Marrakech, UR Protection des Plantes, Avenue Mohammed 6, BP 533, Marrakech, Morocco

    Souad Nour

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  1. Mouaad Amine Mazri
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  2. Ilham Belkoura
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Correspondence to Mouaad Amine Mazri.

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Mazri, M.A., Belkoura, I., Meziani, R. et al. Somatic embryogenesis from bud and leaf explants of date palm (Phoenix dactylifera L.) cv. Najda. 3 Biotech 7, 58 (2017). https://doi.org/10.1007/s13205-017-0676-y

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