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Callogenesis and rhizogenesis in date palm leaf segments: are there similarities between the two auxin-induced pathways?

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Abstract

In date palm (Phoenix dactylifera L. cv. Ahmar, Arecaceae), as for many monocotyledons, callogenesis is a prerequisite for the initiation of somatic embryogenesis, and requires the presence of auxin in the medium. Immature leaf explants were cultivated in medium supplemented with either 1 or 54 μM 1-naphtaleneacetic acid in order to induce either rhizogenesis or callogenesis. Histological studies performed throughout the culture period established that precocious cell reactivation is similar in both morphogenetic pathways. Early cytological modifications are associated with cell reactivation and are observed in the pluripotent cells of perivascular sheaths. Divergence between the callogenesis and rhizogenesis pathways is observed later, during the subsequent determination and morphological differentiation phases. We established that in date palm, the rhizogenesis and callogenesis pathways are initiated from the same cell type, the ultimate developmental fate depending upon auxin concentration.

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Abbreviations

CIM:

Callus induction medium

FP:

Fascicular parenchyma

NAA:

1-Naphtaleneacetic acid

NBB:

Naphtol blue-black

PAS:

Periodic acid Schiff

RP:

Root primordium

RIM:

Root induction medium

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Acknowledgments

H. Saïd-Ahmed gratefully acknowledges grant from EGIDE-Ministère des affaires étrangères (France) for his Master’s degree. B. Gueye and D. Sané were supported by grants from the department for capacity-building support to scientific communities in the South (DSF, Institut de Recherche pour le Développement-IRD). Histological sections of nodular callus and root/root-like structure at d63 were kindly provided by Mrs M. Collin (IRD). F. Aberlenc-Bertossi (IRD) is also thanked for her comments on oil and date palms histology. The authors are indebted to Prof S. Hawkins (UMR 1281, USTL) for his critical reading of the manuscript, valuable discussion and checking English language.

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Authors and Affiliations

  1. Faculté des Sciences et Techniques, Laboratoire de Biotechnologies Végétales, Université Cheikh Anta Diop, BP 5005, Dakar, Sénégal

    B. Gueye & D. Sané

  2. UMR USTL, INRA 1281 Stress abiotiques et différenciation des végétaux cultivés, Université des Sciences et Technologies de Lille, Bâtiment SN2, 3ème étage, 59650, Villeneuve d’Ascq Cedex, France

    H. Saïd-Ahmed, J.-L. Hilbert & A.-S. Blervacq

  3. Centre d’Etudes et de Recherches de Djibouti, CERD-ISV, Route de l’aéroport, BP 486, Djibouti, République de Djibouti

    H. Saïd-Ahmed

  4. CIRAD, UMR DIAPC, Palm Development Group, 34394, Montpellier, France

    F. Morcillo

  5. IRD, UMR DIAPC, Palm Development Group, 34394, Montpellier, France

    A. Borgel

  6. CIRAD, UMR DAP, Plateau d’Histologie et d’Imagerie cellulaire Végétale (PHIV), 34398, Montpellier, France

    J.-L. Verdeil

Authors
  1. B. Gueye
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  2. H. Saïd-Ahmed
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  3. F. Morcillo
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  4. A. Borgel
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  5. D. Sané
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  6. J.-L. Hilbert
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  7. J.-L. Verdeil
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  8. A.-S. Blervacq
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Corresponding author

Correspondence to A.-S. Blervacq.

Additional information

B. Gueye and H. Saïd-Ahmed have contributed equally.

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Gueye, B., Saïd-Ahmed, H., Morcillo, F. et al. Callogenesis and rhizogenesis in date palm leaf segments: are there similarities between the two auxin-induced pathways?. Plant Cell Tiss Organ Cult 98, 47–58 (2009). https://doi.org/10.1007/s11240-009-9537-7

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  • DOI: https://doi.org/10.1007/s11240-009-9537-7

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