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Foliar spray of Auxin/IAA modulates photosynthesis, elemental composition, ROS localization and antioxidant machinery to promote growth of Brassica juncea

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Abstract

Auxins (Aux) are primary growth regulators that regulate almost every aspect of growth and development in plants. It plays a vital role in various plant processes besides controlling the key aspects of cell division, cell expansion, and cell differentiation. Considering the significance of Aux, and its potential applications, a study was conducted to observe the impact of indole acetic acid (IAA), a most active and abundant form of Aux on Brassica juncea plants growing under natural environmental conditions. Different concentrations (0, 10−10, 10−8, 10−6 M) of IAA were applied once in a day at 25-day stage of growth for 5 days, consecutively. Various parameters (growth, photosynthetic, biochemical, oxidative biomarkers and nutrient composition) were assessed at different days after sowing (DAS). Scanning electron microscopy (SEM) of leaf stomata, reactive oxygen species (ROS) localization in leaf and roots, and confocal microscopy were also conducted. The results revealed that all the IAA concentrations were effective in growth promotion and ROS reduction, however, the 10−8 M of IAA exhibited the maximum improvement in all the above mentioned parameters as compared to the control.

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Acknowledgements

The authors are thankful to the Aligarh Muslim University for providing all the required facilities during the experiment and Anayat Rasool Mir is also thankful to the University Grant Commission, New Delhi, India for the award of Non-Net Fellowship.

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  1. Plant Physiology Section, Department of Botany, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, India

    Anayat Rasool Mir, Husna Siddiqui & Shamsul Hayat

  2. Department of Biology, College of Science and Humanities, Prince Sattam bin Abdulaziz University, Al-Kharj, 11942, Saudi Arabia

    Pravej Alam

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  1. Anayat Rasool Mir
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Mir, A.R., Siddiqui, H., Alam, P. et al. Foliar spray of Auxin/IAA modulates photosynthesis, elemental composition, ROS localization and antioxidant machinery to promote growth of Brassica juncea. Physiol Mol Biol Plants 26, 2503–2520 (2020). https://doi.org/10.1007/s12298-020-00914-y

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