Summary
Fresh leaf discs/pieces of wheat, gram, maize and mustard were incubated in a substrate consisting of 4 ml 0.2M phosphate buffer (pH 6.8), 4 ml 0.2M NaHCO3 and 0.2 ml 0.002% bromthymol blue at 0 to 4°C for 120–240 seconds. The conversion of light blue colour due to the activity of zinc dependent carbonic anhydrase into yellowish green and greenish yellow was used as an index for zinc deficiency and sufficiency, respectively, in the plant tissues. A correlation coefficient value of r=>+0.9 between the different parameters of rapid tissue test and standard enzyme extraction method, indicated the feasibility of using the present method. re]19730724
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References
Bar-Akiva, A., and Lavon, R., J. Hort. Sci. 44, 345–62 (1969).
Dwivedi, R., Snehi, and Takkar, P. N., Plant and Soil 39 (in press) (1973).
Keilin, D. and Mann, T., Biochem. J. 34, 116–76 (1940).
Kessler, B., Plant Analysis and Fertilizer Problems ed. Water Rurthor. Am. Inst. Sci. Washington Publ. No. 8 (1961).
Lindskog, S., and Malmstrom, B. G., Biochem. Biophys. Research Comm. 2, 213–17 (1960).
Lowry, O. H. et al., J. Biol. Chem. 193, 265–75 (1951).
Handbook. Punjab Agricultural University, Ludhiana Indian (1973).
Sibly, P. M., and Wood, J. G., Australian J. Sci. Research 4, 500–10 (1951).
Wood, J. G., and Sibly, P. M., Australian J. Sci. Research B. 5, 244–55 (1952).
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Dwivedi, R.S., Randhawa, N.S. Evaluation of a rapid test for the hidden hunger of zinc in plants. Plant Soil 40, 445–451 (1974). https://doi.org/10.1007/BF00011531
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DOI: https://doi.org/10.1007/BF00011531