Abstract
1,3-Propanediol (1,3-PDO) has numerous industrial applications in the synthesis of the monomer of the widely used fiber polytrimethylene terephthalate. In this work, the production of 1,3-PDO by Klebsiella pneumoniae is increased by dual-substrate cultivation and fed-batch fermentation. Experimental results indicate that the production of 1,3-PDO can be elevated to 16.09 g/L using a dual substrate ratio (of glucose to crude glycerol) of 1/30 and to 20.73 g/L using an optimized dual-substrate ratio of 1/20. Ultimately, the optimal dual-substrate feeding for a 5 L scale fed-batch fermenter that maximizes 1,3-PDO production (29.69 g/L) is determined. This production yield is better than that reported in most related studies. Eventually, the molecular weight and chemical structure of 1,3-PDO were obtained by FAB-MS, 1H-NMR, and 13C-NMR. Also, in demonstrating the effectiveness of the fermentation strategy in increasing the production and production yield of 1,3-PDO, experimental results indicate that the fermentation of 1,3-PDO is highly promising for commercialization.
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The authors gratefully acknowledge the financial support of the Ministry of Science and Technology of the Republic of China, provided under grant numbers MOST 107-2221-E-155-023-MY3 and MOST 107-2218-E-155 -001.
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Prof. Yu-Hong Wei were responsible for the overall design and investigation. Wei-Chuan Chen and Chi-Ju Chuang were responsible for the experimental performances. Prof. Jo-Shu Chang, Prof. Li-Fen Wang, Prof. Po-Chi Soo, Prof. Ho-Shing Wu, and Prof. Shen-Long Tsai provided the concept of this study. All authors read and approve the final manuscript.
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The presenting author of this manuscript in ACB 2019 is Dr. Wei-Chuan Chen.
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Chen, WC., Chuang, CJ., Chang, JS. et al. Exploring Dual-Substrate Cultivation Strategy of 1,3-Propanediol Production Using Klebsiella pneumoniae. Appl Biochem Biotechnol 191, 346–359 (2020). https://doi.org/10.1007/s12010-019-03208-6
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DOI: https://doi.org/10.1007/s12010-019-03208-6