Abstract
Addition of 5 mm fumarate to cultures of Klebsiella pneumoniae enhanced the rate of glycerol consumption and the production of 1,3-propanediol (PDO). Compared to the control, the activity of glycerol dehydrogenase increased by 35, 33 and 46%, the activity of glycerol dehydratase increased by 160, 210 and 115%, and the activity of 1,3-propanediol oxidoreductase increased by 25, 39 and 85% when, respectively, 5, 15 and 25 mm fumarate were provided. At the same time, the ratio of NAD+ to NADH decreased by 20, 23 and 29%. Using a 5 l bioreactor with 5 mM fumarate addition, the specific rate of glycerol consumption and the productivity of PDO was 30 mmol/l h and 17 mmol/l h, respectively, both increased by 35% over the control.
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References
M Ailion TA Bobik JR Roth (1993) ArticleTitleTwo global regulatory systems (Crp and Arc) control the cobalamin/propanediol regulon of Salmonella typhimurium J.Bacteriol. 175 7200–7208 Occurrence Handle8226666 Occurrence Handle1:CAS:528:DyaK2cXislajuw%3D%3D
DI Andersson JR Roth (1989) ArticleTitleRedox regulation of the genes for cobinamide biosynthesis in Salmonella typhimurium J.Bacteriol. 171 6734–6739 Occurrence Handle2687249 Occurrence Handle1:CAS:528:DyaK3cXlt1Snuw%3D%3D
F Barbirato S Astruc P Soucaille C Camarasa JM Salmon A Bories (1997) ArticleTitleAnaerobic pathways of glycerol dissimilation by Enterobacter agglomerans CNCM1210: limitations and regulations Microbiology 143 2423–2432 Occurrence Handle9245823 Occurrence Handle1:CAS:528:DyaK2sXkvFKjs7w%3D Occurrence Handle10.1099/00221287-143-7-2423
H Biebl K Menzel AP Zeng WD Deckwer (1999) ArticleTitleMicrobial production of 1,3-propanediol Appl. Microbiol. Biotechnol. 52 289–297 Occurrence Handle10.1007/s002530051523 Occurrence Handle10531640 Occurrence Handle1:CAS:528:DyaK1MXmvVOgur4%3D
H Biebl AP Zeng K Menzel WD Deckwer (1998) ArticleTitleFermentation of glycerol to 1,3-propanediol and 2,3-butanediol by Klebsiella pneumoniae Appl. Microbiol. Biotechnol. 50 24–29 Occurrence Handle10.1007/s002530051251 Occurrence Handle9720196 Occurrence Handle1:CAS:528:DyaK1cXls12gtbc%3D
X Chen DJ Zhang WT Qi SJ Gao ZL Xiu P Xu (2003) ArticleTitleMicrobial fed-batch production of 1,3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions Appl. Microbiol. Biotechnol. 63 143–146 Occurrence Handle10.1007/s00253-003-1369-5 Occurrence Handle12908084 Occurrence Handle1:CAS:528:DC%2BD3sXpsVOjt70%3D
KK Cheng DH Liu Y Sun WB Liu (2004) ArticleTitle1,3-Propanediol production by Klebsiella pneumoniae under different aeration strategies Biotechnol. Lett. 26 911–915 Occurrence Handle10.1023/B:bile.0000025902.44458.f4 Occurrence Handle15269540 Occurrence Handle1:CAS:528:DC%2BD2cXkvVyktLY%3D
RG Forage ECC Lin (1982) ArticleTitle Dha system mediating aerobic and anaerobic dissimilation of glycerol in Klebsiella pneumoniae NCIB 418 J. Bacteriol. 151 591–599 Occurrence Handle6284704 Occurrence Handle1:CAS:528:DyaL38XlsVWntr8%3D
S Honda T Toraya S Fukui (1980) ArticleTitle In situ reactivation of glycerol inactivated coenzyme B12-dependent enzymes, glycerol dehydratase and diol dehydratase J. Bacteriol. 143 1458–1465 Occurrence Handle6997273 Occurrence Handle1:CAS:528:DyaL3cXlvF2qu74%3D
K Menzel K Ahrens AP Zeng WD Deckwer (1998) ArticleTitleKinetic, dynamic, and pathway studies of glycerol metabolism by Klebsiella pneumoniae in anaerobic continuous culture: IV.enzymes and fluxes of glycerol dissimilation and 1,3-propanediol formation Biotechnol. Bioeng. 60 617–626 Occurrence Handle10.1002/(SICI)1097-0290(19981205)60:5<617::AID-BIT12>3.0.CO;2-L Occurrence Handle10099470 Occurrence Handle1:CAS:528:DyaK1cXntFels7s%3D
PJ Slininger RJ Bothast (1985) ArticleTitleOptimizing aerobic conversion of glycerol to 3-hydroxypropionaldehyde Appl. Environ. Microbiol. 50 1444–1450 Occurrence Handle3911907 Occurrence Handle1:CAS:528:DyaL28XjtlSjtg%3D%3D
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Revisions requested 15 July 2005; Revisions received 30 August 2005
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Lin, R., Liu, H., Hao, J. et al. Enhancement of 1,3-propanediol Production by Klebsiella pneumoniae with Fumarate Addition. Biotechnol Lett 27, 1755–1759 (2005). https://doi.org/10.1007/s10529-005-3549-0
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DOI: https://doi.org/10.1007/s10529-005-3549-0