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Screening beneficial rhizobacteria from Spartina maritima for phytoremediation of metal polluted salt marshes: comparison of gram-positive and gram-negative strains

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Abstract

The aim of our work was the isolation and characterization of bacteria from the rhizosphere of Spartina maritima in the metal contaminated Odiel estuary (Huelva, SW Spain). From 25 strains, 84 % were identified as gram-positive, particularly Staphylococcus and Bacillus. Gram-negative bacteria were represented by Pantoea and Salmonella. Salt and heavy metal tolerance, metal bioabsorption, plant growth promoting (PGP) properties, and biofilm formation were investigated in the bacterial collection. Despite the higher abundance of gram-positive bacteria, gram-negative isolates displayed higher tolerance toward metal(loid)s (As, Cu, Zn, and Pb) and greater metal biosorption, as deduced from ICP-OES and SEM-EDX analyses. Besides, they exhibited better PGP properties, which were retained in the presence of metals and the ability to form biofilms. Gram-negative strains Pantoea agglomerans RSO6 and RSO7, together with gram-positive Bacillus aryabhattai RSO25, were selected for a bacterial consortium aimed to inoculate S. maritima plants in metal polluted estuaries for phytoremediation purposes.

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Acknowledgments

This work has been financed by Junta de Andalucía (Proyecto de Excelencia P11-RNM-7274). K. Paredes-Páliz has been supported by a pre-doctoral grant (SENESCYT, Ecuador). The students Rafael Gil-Baena, Yoely Lantigua, and Reyes Serrano are acknowledged for their collaboration in the isolation and preliminary characterization of the strains. Thanks are given to the Microscopy Service of the CITIUS (University of Sevilla, Spain) and particularly to Cristina Vaquero for her help in the preparation and analysis of samples by SEM-EDX.

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  1. Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, c/Profesor García González, 2, 41012, Sevilla, Spain

    Karina I. Paredes-Páliz, Miguel A. Caviedes, Bouchra Doukkali, Ignacio D. Rodríguez-Llorente & Eloísa Pajuelo

  2. Departamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, c/Profesor García González, 4, 41012, Sevilla, Spain

    Enrique Mateos-Naranjo

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  1. Karina I. Paredes-Páliz
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Paredes-Páliz, K.I., Caviedes, M.A., Doukkali, B. et al. Screening beneficial rhizobacteria from Spartina maritima for phytoremediation of metal polluted salt marshes: comparison of gram-positive and gram-negative strains. Environ Sci Pollut Res 23, 19825–19837 (2016). https://doi.org/10.1007/s11356-016-7184-1

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  • DOI: https://doi.org/10.1007/s11356-016-7184-1

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