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Production of indole-3-acetic acid, aromatic amino acid aminotransferase activities and plant growth promotion by Pantoea agglomerans rhizosphere isolates

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Abstract

The production of auxins, such as indole-3-acetic acid (IAA), by rhizobacteria has been associated with plant growth promotion, especially root initiation and elongation. Six indole-producing bacteria isolated from the rhizosphere of legumes grown in Saskatchewan soils and identified as Pantoea agglomerans spp. were examined for their ability to promote the growth of canola, lentil and pea under gnotobiotic conditions and for tryptophan (Trp)-dependent IAA production. Five of the isolates enhanced root length, root weight or shoot weight by 15–37% in at least one of the plant species, but isolates 3–117 and 5–51 were most consistent in enhancing plant growth across the three species. Indole concentrations in the rhizosphere of plants grown under gnotobiotic conditions increased in the presence of the rhizosphere isolates and when Trp was added 3 days prior to plant harvest. Isolates 3–117, 5–51 and 5–105 were most effective in increasing rhizosphere indole concentrations. Colony hybridization confirmed that all of the isolates possessed the ipdC gene which codes for a key enzyme in the Trp-dependent IAA synthetic pathway. The activity of amino acid aminotransferase (AAT), catalyzing the first step in the Trp-dependent synthetic pathway, was examined in the presence of Trp and other aromatic amino acids. All of the isolates accumulated Trp internally and released different amounts of IAA. The production of IAA from the isolates was greatest in the presence of Trp, ranging from 2.78 to 16.34 μg mg protein−1 in the presence of 250 μg of Trp ml−1. The specific activity of AAT was correlated with the concentration of IAA produced in the presence of Trp but not when tyrosine (Tyr), phenylalanine (Phe) or aspartate (Asp) was used as a sole nitrogen source. Isolate 3–117, which produced significant concentrations of IAA in the presence and absence of Trp, was able to use aromatic amino acids as sole sources of nitrogen and was most consistent in enhancing the growth of canola, lentil and pea may have potential for development as a plant growth-promoting inoculant.

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Abbreviations

IAA:

indole-3-acetic acid

Trp:

tryptophan

Tyr:

tyrosine

Phe:

phenylalanine

Asp:

aspartate

2-OG:

2-oxoglutarate

AAT:

aromatic amino acid aminotransferase

HPLC:

high-performance liquid chromatography

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Acknowledgements

This work was supported by grants from the Natural Science and Engineering Research Council of Canada (NSERC). We thank R. O’Brien for HPLC analysis.

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  1. Biology and Physical Geography, Irving K. Barber School of Arts and Sciences, University of British Columbia Okanagan, Kelowna, BC, V1V 1V7, Canada

    Elena Sergeeva, Danielle L. M. Hirkala & Louise M. Nelson

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  1. Elena Sergeeva
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  2. Danielle L. M. Hirkala
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  3. Louise M. Nelson
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Correspondence to Louise M. Nelson.

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Responsible Editor: Peter A. H. Bakker.

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Sergeeva, E., Hirkala, D.L.M. & Nelson, L.M. Production of indole-3-acetic acid, aromatic amino acid aminotransferase activities and plant growth promotion by Pantoea agglomerans rhizosphere isolates. Plant Soil 297, 1–13 (2007). https://doi.org/10.1007/s11104-007-9314-5

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