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Relationship between endogenous auxin and cytokinin levels and morphogenic responses inActinidia deliciosa tissue cultures

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Summary

Thein vitro culture ofActinidia deliciosa petioles results in a decline of cytokinin content and an increase of auxin levels. The addition of plant growth regulators (PGRs) to the medium lead to recovery of the initial auxin content, and callus induction occurs at the basal end of the explants. Endogenous auxin/cytokinin ratio was higher at this side than in the apical one, due to unequal distribution of endogenous PGRs in the cultured petioles. Some of the induced calluses showed shoot formation when they were transferred to proliferation medium. Most important differences found in hormonal content between organogenic and non-organogenic callus concerned benzyladenine levels. In this paper the relationships between explant behaviour and their hormonal content is discussed.

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Abbreviations

BM:

basal medium

BA:

6-benzyladenine

CIM:

callus induction medium

CPM:

callus proliferation medium

(diH)Z:

dihydrozeatin

DW:

dry weight

ELISA:

enzyme linked immunoassay

FW:

fresh weight

HPLC:

high performance liquid chromatography

IAA:

indole-3-acetic acid

iP:

N6-(δ2-isopentenyl)adenine

NAA:

1-naphthaleneacetic acid

PBS:

phosphate buffer

PGR:

plant growth regulator

(diH)[9R]Z:

9-β-D-ribofuranosyl-(diH)

[9R]iP:

9-β-Dribofuranosyl-iP

[9R]Z:

9-β-D-ribofuranosylzeatin

TBS:

trishydroxymethyl-aminomethane buffer

Z:

zeatin

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Authors and Affiliations

  1. Lab. Fisiología Vegetal, Dpto. B.O.S., Fac. Biología, Univ. Oviedo, C/ Catedrático Rodrigo Uría, E-33071, Oviedo, Spain

    M. L. Centeno, A. Rodríguez, I. Feito & B. Fernández

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  1. M. L. Centeno
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  2. A. Rodríguez
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  3. I. Feito
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  4. B. Fernández
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Centeno, M.L., Rodríguez, A., Feito, I. et al. Relationship between endogenous auxin and cytokinin levels and morphogenic responses inActinidia deliciosa tissue cultures. Plant Cell Reports 16, 58–62 (1996). https://doi.org/10.1007/BF01275450

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  • DOI: https://doi.org/10.1007/BF01275450

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