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Direct shoot bud induction and plant regeneration in Capsicum frutescens Mill.: influence of polyamines and polarity

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Abstract

Direct shoot bud induction and plant regeneration was achieved in Capsicum frutescens var. KTOC. Aseptically grown seedling explants devoid of roots, apical meristem and cotyledons were inoculated in an inverted position in medium comprising of Murashige and Skoog (Physiol Plant 15:472–497, 1962) basal medium supplemented with 2-(N-morpholine) ethanesulphonic acid buffer along with 2.28 μM indole-3-acetic acid, 10 μM silver nitrate and either of 13.31–89.77 μM benzyl adenine (BA), 9.29–23.23 μM kinetin, 0.91–9.12 μM zeatin, 2.46–9.84 μM 2-isopentenyl adenine. Profuse shoot bud induction was observed only in explants grown on a media supplemented with BA (26.63 μM) as a cytokinin source and 19.4 ± 4.2 shoot buds per explant was obtained in inverted mode under continuous light. Incorporation of polyamine inhibitors in the culture medium completely inhibited shoothoot bud induction. Incorporation of exogenous polyamines improved the induction of shoot buds under 24 h photoperiod. These buds were elongated in MS medium containing 2.8 μM gibberellic acid. Transfer of these shoots to hormone-free MS medium resulted in rooting and rooted plants were transferred to fields. This protocol can be efficiently used for mass propagation and presumably also for regeneration of genetically transformed C. frutescens.

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Abbreviations

ADC:

Arginine decarboxylase

AgNO3 :

Silver nitrate

BA:

N 6-benzyladenine

DFMA:

α-dl-Difluromethyl arginine

DFMO:

α-dl-Difluromethyl ornithine

GA3 :

Gibberellic acid

IAA:

Indole-3-acetic acid

2iP:

Isopentenyladenine

MES:

2-(N-morpholine) ethanesulphonic acid

MS:

Murashige and Skoog medium

PAA:

Phenyl acetic acid

Put:

Putrescine

Spd:

Spermidine

Spm:

Spermine

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Acknowledgments

The authors VK, AS and BCNP gratefully acknowledge Council of Scientific and Industrial Research (CSIR), New Delhi for research fellowships. This research was financially supported by the Department of Biotechnology, New Delhi, India. We are thankful to DRDO, Pithoragarh, Uttaranchal for providing the seeds of C. frutescens var. KTOC.

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Authors and Affiliations

  1. Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore, 570 020, Karnataka, India

    Vinod Kumar, Ashwani Sharma, Bellur Chayapathy Narasimha Prasad, Harishchandra Bhaskar Gururaj, Parvatam Giridhar & Gokare Aswathanarayana Ravishankar

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  1. Vinod Kumar
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  2. Ashwani Sharma
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  3. Bellur Chayapathy Narasimha Prasad
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  4. Harishchandra Bhaskar Gururaj
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  5. Parvatam Giridhar
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  6. Gokare Aswathanarayana Ravishankar
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Corresponding author

Correspondence to Gokare Aswathanarayana Ravishankar.

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Communicated by J. Kepczynski.

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Kumar, V., Sharma, A., Narasimha Prasad, B.C. et al. Direct shoot bud induction and plant regeneration in Capsicum frutescens Mill.: influence of polyamines and polarity. Acta Physiol Plant 29, 11–18 (2007). https://doi.org/10.1007/s11738-006-0002-5

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  • DOI: https://doi.org/10.1007/s11738-006-0002-5

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