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The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds

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Abstract

Bromus auleticus (Trin.) is a grass native to the southern cone with important agronomical potential as fodder. Different breeding programs have been initiated with this grass, but plant tissue culture techniques could not be used because B. auleticus is recalcitrant. The aim of the present study was to develop a micropropagation protocol in the genus Bromus and to investigate if the association between B. auleticus and Epichloë endophytes affected in vitro culture and growth of micropropagated plantlets. In different micropropagation stages, better results were obtained with endophyte-infected (E+) seeds compared to endophyte-free (E−) seeds. The E+ seeds presented higher percentages of in vitro germination (82 ± 5 vs. 57 ± 6%), callus induction (72 ± 6 vs. 37 ± 6%), and plant regeneration from callus (89 ± 5 vs. 13 ± 5%). We also compared the biomass of shoot complexes and regenerated plantlets. After 4 weeks of culture, shoot complexes obtained from E+ seeds reached greater weight than the ones regenerated from E− seeds (173 ± 24 vs. 74 ± 9 mg). More than the 80% of the regenerated shoot complexes were rooted ex vitro and acclimated, regardless of their origin (E+ or E−). Finally, after 4 weeks of acclimatization, the plantlets regenerated from E+ seeds reached a greater weight than the ones from E− seeds, (461 ± 64 vs. 172 ± 25 mg). These results indicate that the use of endophyte-infected (E+) seeds enhances significantly B. auleticus micropropagation and promotes growth of the regenerated plantlets.

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Acknowledgements

This work was supported by funding from Agencia Nacional de Promoción Científica y Tecnológica (PICT Joven 2016-0487, PICT 2016-0877, PICT 2014-3315), CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina) Grant PIP 11220150100956CO and from Universidad de Buenos Aires UBACyT (20020150100067BA and 20020150200075BA).

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Authors and Affiliations

  1. Laboratorio de Cultivo Experimental de Plantas y Microalgas, No. 68, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina

    J. J. Regalado, V. Berdion & S. I. Pitta-Alvarez

  2. Laboratorio de Micología y Fitopatología, No. 69, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina

    V. Berdion, M. V. Vignale, M. V. Novas & L. J. Iannone

  3. Instituto de Micología y Botánica (INMIBO), CONICET - Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina

    J. J. Regalado, M. V. Vignale, M. V. Novas, S. I. Pitta-Alvarez & L. J. Iannone

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  1. J. J. Regalado
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  2. V. Berdion
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  3. M. V. Vignale
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  4. M. V. Novas
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  5. S. I. Pitta-Alvarez
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  6. L. J. Iannone
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Contributions

RJJ and PASI designed the micropropagation experiments. RJJ and BV executed the micropropagation experiments. VMV checked endophytic status of plant material. NMV and ILJ provided the plant material. RJJ wrote the manuscript. VMV, NMV and ILJ reviewed the manuscript. PASI reviewed the English of the manuscript. ILJ supervised the work.

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Correspondence to J. J. Regalado.

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Communicated by Fredy Altpeter.

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Regalado, J.J., Berdion, V., Vignale, M.V. et al. The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds. Plant Cell Tiss Organ Cult 135, 279–286 (2018). https://doi.org/10.1007/s11240-018-1462-1

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  • DOI: https://doi.org/10.1007/s11240-018-1462-1

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