Abstract
The effects of native isolates of Pseudomonas fluorescens, Azospirillum brasilense, and Trichoderma harzianum on rooting and acclimatization of in vitro-grown shoots and plantlets of tea were evaluated. In vitro bacterization of P. fluorescens failed to establish, while both T. harzianum and A. brasilense retarded shoot growth, eventually overtaking shoot cultures in in vitro rooting. Acclimatization of rooted plantlets in soil amended with bioinoculants, either individually or in various combinations, promoted plantlet survival. Moreover, efficiency of nutrient uptake of plantlets was higher in the presence of microorganisms. Root rot or wilting of tissue culture-derived plants was not observed in bioinoculant-treated plants, as they possessed relatively higher activities of defense enzymes, including peroxidase and phenylalanine ammonia lyase.
Abbreviations
- HMS:
-
Half-strength Murashige and Skoog medium
- IBA:
-
Indole 3-butyric acid
- PAL:
-
Phenylalanine ammonia lyase
- PDB:
-
Potato dextrose broth
- PGPR:
-
Plant growth promoting rhizobacteria
- PO:
-
Peroxidase
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Acknowledgments
The authors express their sincere thanks to Dr. P. Mohan Kumar, Director and Dr. N. Murlaeedharan, Advisor of UPASI Tea Research Foundation, Valparai for their support and encouragement during the period of study.
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Thomas, J., Ajay, D., Raj Kumar, R. et al. Influence of beneficial microorganisms during in vivo acclimatization of in vitro-derived tea (Camellia sinensis) plants. Plant Cell Tiss Organ Cult 101, 365–370 (2010). https://doi.org/10.1007/s11240-010-9687-7
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DOI: https://doi.org/10.1007/s11240-010-9687-7