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Investigation of roles of IL-8 (+ 781 C/T) and MMP-2 (-735 C/T) gene variations in early diagnosis of bladder cancer and progression

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Abstract

Background

The aim of our study is to investigate the roles of IL-8 (+ 781 C/T) and MMP-2 (-735 C/T) gene variations in early diagnosis and progression of BCA.

Methods

Polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) methods were used to determine the genotype distributions of IL-8 (+ 781 C/T) and MMP-2 (-735 C/T) gene variations.

Results

In our study, the genotype distributions of IL-8 (+ 781 C/T) and MMP-2 (-735 C/T) gene variations were not found to be significantly different between the patient and control groups. In addition, C and T allele frequencies for these gene variations were not different from the Hardy-Weinberg distribution in patient and control groups. However, when the combined genotype analyzes for these gene variations were evaluated, CC-CC and CT-CC combined genotypes for + 781 C/T / -735 C/T gene variations were observed significantly more in the patient group compared to other genotypes.

Conclusion

Although IL-8 (+ 781 C/T) and MMP-2 (-735 C/T) gene variations were not found to be genetic risk factors in the Thrace population in our study, CC-CC and CT-CC combined genotypes were determined as genetic risk factors for BCA susceptibility. The combined genotypes obtained as a result of the combined genotype analysis of these genetic variations that are effective in tumor progression may be considered to be important biomarkers for the early diagnosis and progression of BCA.

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Acknowledgements

This study was performed at departments of Biophysics and Urology in Trakya University Faculty of Medicine.

Funding

This study was not supported by any specific grant from funding.

Author information

Authors and Affiliations

  1. Department of Biophysics, Faculty of Medicine, Haliç University, Istanbul, Turkey

    Nevra Alkanli

  2. Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, Turkey

    Arzu Ay

  3. Department of Urology, Faculty of Medicine, Trakya University, Edirne, Turkey

    Gokhan Cevik

Authors
  1. Nevra Alkanli
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  2. Arzu Ay
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  3. Gokhan Cevik
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by [Nevra Alkanli], [Arzu Ay], and [Gokhan Cevik]. The first draft of the manuscript was written by [Nevra Alkanli and Arzu Ay] and all authors commented on previous versions of the manuscript.

Corresponding author

Correspondence to Nevra Alkanli.

Ethics declarations

Ethical approval

For our study, ethics committee approval was obtained with the TÜTF-BAEK 2020/387 protocol code from Trakya University Faculty of Medicine Non-Invasive Clinical Research Ethics Committee.

Consent to participate

Signed informed consent form was collected from each individual in the patient group with BCA and healthy control group.

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The authors declare that they have no conflict of interest.

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Alkanli, N., Ay, A. & Cevik, G. Investigation of roles of IL-8 (+ 781 C/T) and MMP-2 (-735 C/T) gene variations in early diagnosis of bladder cancer and progression. Mol Biol Rep 50, 443–451 (2023). https://doi.org/10.1007/s11033-022-07881-5

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  • DOI: https://doi.org/10.1007/s11033-022-07881-5

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