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Gene expression analysis of intestinal IL-8, IL-17 A and IL-10 in patients with celiac and inflammatory bowel diseases

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A Correction to this article was published on 24 April 2024

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Abstract

Background

Celiac disease (CeD) and inflammatory bowel disease (IBD) are accompanied by impaired immune responses. To study the immune regulation of these diseases, we evaluated the expression levels of pro-inflammatory (IL-8 and IL-17 A) and anti-inflammatory (IL-10) cytokines in intestinal biopsy specimens of CeD and IBD patients in comparison to healthy subjects.

Methods and Results

Intestinal biopsies were collected from 33 patients with IBD, 47 patients with CeD, and 20 healthy individuals. Total RNA was extracted and mRNA expression levels of IL-8, IL-17 A and IL-10 were assessed by qPCR. P-value < 0.05 was considered statistically significant. The expression levels of IL-8 and IL-17 A were higher in biopsies of IBD (UC and CD) and CeD patients compared to the control group (P < 0.05). IBD patients (UC and CD) had higher IL-8 intestinal level than CeD patients (P < 0.0001 and P = 0.0007, respectively). The expression of IL-10 was significantly down-regulated in intestinal biopsies of CeD and IBD patients compared with controls (P < 0.001). In addition, the expression level of this cytokine was significantly lower in IBD patients (P < 0.001 for UC patients and P < 0.0001 for CD patients) than CeD group.

Conclusions

The three selected pro- and anti-inflammatory cytokines showed a similar expression pattern in both IBD and CeD patients. As IBD and CeD are immune-mediated disorders and are accompanied by inflammatory events, the understanding of the similarities and differences among them can help researchers to find out useful candidate therapeutic protocols. We suggest that larger cohort studies be organized to achieve more insights into this regulation.

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Acknowledgements

The Research Institute for Gastroenterology and Liver Diseases (RIGLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran is gratefully acknowledged.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Elham Aghamohamadi, Nastaran Asri †, Vahid Chaleshi, Yasaman Hajinabi, Fahimeh Sadat Gholam-Mostafaei & Mohammad Reza Zali

  2. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Aylin Odak, Mohammad Rostami-Nejad & Hamid Asadzadeh-Aghdaei

  3. Applied Biotechnology Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, 1949635881, Iran

    Maryam Eslami

  4. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, 1949635881, Iran

    Maryam Eslami

  5. Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran

    Saeed Mohammadian Haftcheshmeh

  6. Bambino Gesù Children’s Hospital-IRCCS, Research Laboratories, V.le San Paolo 15, 00146, Rome, Italy

    Andrea Masotti

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  1. Elham Aghamohamadi
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Contributions

Elham Aghamohamadi, Nastaran Asri, Aylin Odak, Yasaman Hajinabi, Fahimeh Sadat Gholam-Mostafaei and Saeed Mohammadian Haftcheshmeh: Methodology, Investigation, Writing- Original draft preparation; Elham Aghamohamadi: Software, Data curation; Mohammad Rostami-Nejad, Andrea Masotti, Vahid Chaleshi, Hamid Asadzadeh-Aghdaei, Maryam Eslami and Mohammad Reza Zali: Reviewing and Editing. Mohammad Rostami-Nejad: designed the work plan. Mohammad Rostami-Nejad: Conceptualization, Supervision.

Corresponding authors

Correspondence to Mohammad Rostami-Nejad or Andrea Masotti.

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This study was approved by the ethical committee of the Research Institute for Gastroenterology and Liver Diseases (RIGLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran IR.SBMU.RIGLD.REC.1396.156.

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The original online version of this article was revised: The affiliation details for author ‘Maryam Eslami’ was incorrectly given as ‘Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran’ but should have been ‘Applied Biotechnology Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran 1949635881, Iran’ and ‘Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran 1949635881, Iran’.

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Aghamohamadi, E., Asri †, N., Odak, A. et al. Gene expression analysis of intestinal IL-8, IL-17 A and IL-10 in patients with celiac and inflammatory bowel diseases. Mol Biol Rep 49, 6085–6091 (2022). https://doi.org/10.1007/s11033-022-07397-y

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