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Development of a robust marker for Psy-1 homoeologs and its application in improvement of yellow pigment content in durum wheat

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Abstract

Phytoene synthase-1 (Psy-1) homoeologs are associated with yellow pigment content (YPC) in endosperm of durum and bread wheat. In the present study, microsatellite variation in promoter region of Psy-A1 was identified in durum wheat and marker Psy-1SSR, targeting the microsatellite variation was developed which amplifies variation in Psy-A1 and Psy-B1 loci simultaneously. Psy-A1SSR was mapped within QYp.macs-7A, a major QTL for YPC identified earlier in PDW 233/Bhalegaon 4 population. Marker Psy-A1SSR was further validated in two different RIL populations and a set of 222 tetraploid wheat accessions including less cultivated tetraploid wheat species. Eight alleles of Psy-A1SSR were identified in 222 wheat accessions, while seven alleles were observed for Psy-B1SSR. Variation at Psy-A1SSR showed significant association with YPC, whereas no association was observed with Psy-B1SSR. Marker-assisted introgression of Psy-A1SSRe allele from PDW 233, to durum wheat cultivars MACS 3125 and HI 8498 resulted in improvement of YPC. Backcrossed BC3F2:4 and BC2F2:3 lines selected using Psy-A1SSR showed 89 to 98% gain in YPC over recurrent parents indicating robustness of marker. The marker can thus be utilized in marker-assisted improvement of YPC in durum wheat cultivars.

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Funding

This study was financially supported by the Department of Biotechnology, Ministry of Science & Technology, Govt. of India, vide Grant No. BT/PR11695/AGR/02/643/2008.

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  1. Genetics and Plant Breeding Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, 411004, India

    Ravindra Patil, Manoj Oak, Anuja Deshpande & Shubhada Tamhankar

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  1. Ravindra Patil
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  2. Manoj Oak
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Correspondence to Shubhada Tamhankar.

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Patil, R., Oak, M., Deshpande, A. et al. Development of a robust marker for Psy-1 homoeologs and its application in improvement of yellow pigment content in durum wheat. Mol Breeding 38, 136 (2018). https://doi.org/10.1007/s11032-018-0895-x

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