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Improving the yellow pigment content of bread wheat flour by selecting the three homoeologous copies of Psy1

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Abstract

The yellow pigment content (YPC) of endosperm affects the quality and nutritional value of wheat grain products. Major quantitative trait loci (QTL) for endosperm YPC have been repeatedly mapped on chromosomes 7A and 7B in durum and bread wheats. The genes coding for phytoene synthase (PSY1), which is involved in the biosynthesis of carotenoids, generally co-segregate with these QTL, indicating their role in determining YPC. Here, to study the genetic factors underlying endosperm YPC in bread wheat, the sequence polymorphism of the homoeologous A, B and D copies of genes coding for PSY1, Psy-A1, Psy-B1, and Psy-D1, was studied in a worldwide core collection, which was also phenotyped for flour YPC. Seven novel alleles of Psy-A1 and two novel alleles of Psy-B1 were detected, which confirms the high level of polymorphism of these genes. Two major QTL with respective candidate genes Psy-A1 and Psy-B1 were identified in the distal region of chromosomes 7A and 7B using progeny of a cross between Apache and Ornicar, high and low YPC cultivars, respectively. Association mapping confirms the role of these genes in YPC and shows that the D copy also significantly influences this trait. These results indicate that breeders need to consider all three Psy1 copies when seeking to improve the YPC of wheat endosperm.

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References

  • Adom KK, Sorrels ME, Liu RH (2003) Phytochemical profiles and antioxidant activity of wheat varieties. J Agric Food Chem 51:7825–7834

    Article  PubMed  CAS  Google Scholar 

  • Akhunov E, Nicolet C, Dvorak J (2009) Single nucleotide polymorphism genotyping in polyploid wheat with the Illumina GoldenGate assay. Theor Appl Genet 119:507–517

    Article  PubMed  CAS  Google Scholar 

  • Akhunov E, Akhunova AR, Anderson OD, Anderson JA, Blake N et al (2010) Nocleotide diversity maps reveal variation in diversity among wheat genomes and chromosomes. BMC Genomics 11:702

    Article  PubMed  CAS  Google Scholar 

  • Badaeva ED, Dedkova OS, Gay G, Pukhalskyi VA, Zelenin AV, Bernard S, Bernard M (2007) Chromosomal rearrangements in wheat: their type and distribution. Genome 50:907–926

    Article  PubMed  CAS  Google Scholar 

  • Balfourier F, Roussel V, Strelchenko P, Exbrayat-Vinson F, Sourdille P, Boutet G, Koenig J, Ravel C, Mitrofanova O, Beckert M, Charmet G (2007) A worldwide bread wheat core collection restricted to a full 384 deep well storage plate. Theor Appl Genet 114:1265–1275

    Article  PubMed  Google Scholar 

  • Bordes J, Ravel C, Le Gouis J, Lapierre A, Charmet G, Balfourier F (2011) Use of a global wheat core collection for association analysis of flour and dough quality traits. J Cereal Sci 54:137–147

    Article  Google Scholar 

  • CIE (Commission Internationale de l’Eclairage) (1986) Publication 152 colorimetry, 2nd edn. CIE Central Bureau Kegelgasse, Wien

  • Clarke FR, Clarke JM, McCaig TN, Knox RE, DePauw RM (2006) Inheritance of yellow pigment in concentration in four durum wheat crosses. Can J Plant Sci 86:133–141

    Article  Google Scholar 

  • Crawford AC, Stefanova K, Lambe W, McLean R, Wilson R, Barclay I, Francki MG (2011) Functional relationships of phytoene synthase 1 alleles on chromosome 7A controlling flour colour variation in selected Australian wheat genotypes. Theor Appl Genet 123:95–108

    Article  PubMed  CAS  Google Scholar 

  • Dibari B, Murat F, Chosson A, Gautier V, Poncet C, Lecomte P, Mercier I, Bergès H, Pont C, Blanco A, Salse J (2012) Deciphering the genomic structure, function and evolution of carotenogenesis related phytoene synthase in grasses. BMC Genomics 13:221

    Article  PubMed  CAS  Google Scholar 

  • Digesù AM, Platani C, Cattivelli L, Mangini G, Blanco A (2009) Genetic variability in yellow pigment components in cultivated and wild tetraploid wheats. J Cereal Sci 50:210–218

    Article  Google Scholar 

  • Elouafi I, Nachit MM, Martin LM (2001) Identification of a micro-satellite on chromosome 7B showing a strong linkage with yellow pigment in durum wheat. Hereditas 135:255–261

    Article  PubMed  CAS  Google Scholar 

  • Farré G, Bai C, Twyman RM, Capell T, Christou P, Zhu C (2011) Nutritious crops producing multiple carotenoids—a metabolic balancing act. Trends Plant Sci 16:532–540

    Article  PubMed  Google Scholar 

  • Gallagher CE, Matthews PD, Li F, Wurtel ET (2004) Gene duplication in the carotenoid biosynthetic pathway preceded evolution of the grasses. Plant Cell 135:1776–1783

    CAS  Google Scholar 

  • Gouesnard B, Bataillon T, Decoux G, Rozale C, Schoen DL, David JL (2001) An algorithm for building germplasm core collections by maximizing allelic or phenotypic richness. J Hered 92:93–94

    Article  PubMed  CAS  Google Scholar 

  • Guo AY, Zhu QH, Chen X, Luo JC (2007) GSDS: a gene structure display server. Yi Chuan 29:1023–1026

    Article  PubMed  CAS  Google Scholar 

  • Haseneyer G, Ravel C, Dardevet M, Balfourier F, Sourdille S, Charmet G, Brunel D, Sauer S, Geiger HH, Graner A, Stracke S (2008) High level of conservation between genes coding for the GAMYB transcription factor in barley (Hordeum vulgare L.) and bread wheat (Triticum aestivum L.) collections. Theor Appl Genet 117:321–331

    Article  PubMed  CAS  Google Scholar 

  • He XY, Zhang YL, He ZH, Wu YP, Xiao YG, Ma CX, Xia XC (2008) Characterization of phytoene synthase 1 gene (Psy1) located on common wheat chromosome 7A and development of a functional marker. Theor Appl Genet 116:213–221

    Article  PubMed  CAS  Google Scholar 

  • He XY, He ZH, Ma W, Appels R, Xia XC (2009) Allelic variants of phytoene synthase 1 (Psy1) genes in Chinese and CIMMYT wheat cultivars and development of functional markers for flour colour. Mol Breed 23:553–563

    Article  CAS  Google Scholar 

  • Hentschel V, Kranl K, Hollmann J, Lindhauer MG, Bohmand V, Bitsch R (2002) Spectrophotometric determination of yellow pigment content and evaluation of carotenoids by high-performance liquid chromatography in durum wheat grain. J Agric Food Chem 50:6663–6666

    Article  PubMed  CAS  Google Scholar 

  • Horvath A, Didier A, Koenig J, Exbrayat F, Charmet G, Balfourier F (2009) Analysis of diversity and linkage disequilibrium along the chromosome 3B of bread wheat (Triticum aestivum L.). Theor Appl Genet 119:1523–1537

    Article  PubMed  CAS  Google Scholar 

  • Howitt C, Cavanagh C, Bowerman A, Cazzonelli C, Rampling L, Mimica J, Pogson B (2009) Alternative splicing, activation of cryptic exons and amino acid substitutions in carotenoid biosynthetic genes are associated with lutein accumulation in wheat endosperm. Funct Integr Genomics 9:363–376

    Article  PubMed  CAS  Google Scholar 

  • Humphries JM, Khachik F (2003) Distribution of lutein, zeaxanthin, and related geometrical isomers in fruit, vegetables, wheat, and pasta products. J Agric Food Chem 51:1322–1327

    Article  PubMed  CAS  Google Scholar 

  • Korol AB, Ronin YI, Nevo E (1998) Approximate analysis of QTL–environment interaction with no limits on the number of environments. Genetics 148:2015–2028

    PubMed  CAS  Google Scholar 

  • Kuchel H, Langridge P, Mosionek L, Williams K, Jefferies SP (2006) The genetic control of milling yield, dough rheology and baking quality of wheat. Theor Appl Genet 112:1487–1495

    Article  PubMed  CAS  Google Scholar 

  • Lander ES, Geen P, Abrahanson J, Barlow A, Daly MJ, Lincoln SE, Newbur L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Laurie DA, Bennett MD (1988) The production of haploid wheat plants from wheat × maize crosses. Theor Appl Genet 76:393–397

    Article  Google Scholar 

  • Leenhardt F, Lyan B, Rock E, Boussard A, Potus J, Chanliaud E, Remesy C (2006) Genetic variability of carotenoid concentration, and lipoxygenase and peroxidase activities among cultivated wheat species and bread wheat varieties. Eur J Agron 25:170–176

    Article  CAS  Google Scholar 

  • Li F, Vallabhaneni R, Wurtzel ET (2008a) Psy3, a new member of the phytoene synthase gene family conserved in the Poaceae and regulator of abiotic stress-induced root carotenogenesis. Plant Physiol 46:1333–1345

    Article  Google Scholar 

  • Li F, Vallabhaneni R, Yu J, Rochefort T, Wurtzel ET (2008b) The maize phytoene synthase gene family: overlapping roles for carotenogenesis in endosperm, photomorphogenesis, and thermal stress tolerance. Plant Physiol 147:1334–1346

    Article  PubMed  CAS  Google Scholar 

  • Mares DJ, Campbell AW (2001) Mapping components of flour and noodle colour in Australian wheat. Aust J Agric Res 52:1297–1309

    Article  CAS  Google Scholar 

  • Moran N, Jarvik T (2010) Lateral transfer of genes from fungi underlies carotenoid production in aphids. Science 328:624–627

    Article  PubMed  CAS  Google Scholar 

  • Nicot N, Chiquet V, Gandon B, Amilhat L, LeGeai F, Leroy P, Bernard M, Sourdille P (2004) Study of simple sequence repeat (SSR) markers from wheat expressed sequence tags (ESTs). Theor Appl Genet 109:800–805

    Article  PubMed  CAS  Google Scholar 

  • Parker GD, Chalmers KJ, Rathjen AJ, Landridge P (1998) Mapping loci associated with flour colour in wheat. Theor Appl Genet 97:238–245

    Article  CAS  Google Scholar 

  • Pozniak CJ, Knox RE, Clarke FR, Clarke JM (2007) Identification of QTL and association of a phytoene synthase gene with endosperm colour in durum wheat. Theor Appl Genet 114:525–537

    Article  PubMed  CAS  Google Scholar 

  • Ravel C, Praud S, Murigneux A, Canaguier A, Sapet F, Samson D, Balfourier F, Dufour P, Chalhoub B, Brunel D, Beckert M, Charmet G (2006) Single-nucleotide polymorphisms (SNPs) frequency in a set of selected lines of bread wheat (Triticum aestivum L.). Genome 49:1131–1139

    Article  PubMed  CAS  Google Scholar 

  • Ravel C, Martre P, Romeuf I, Dardevet M, El-Malki R, Bordes J, Duchateau N, Brunel D, Balfourier F, Charmet G (2009) Nucleotide polymorphism in the wheat transcriptional activator Spa influences its pattern of expression and has pleiotropic effects on grain protein composition, dough viscoelasticity, and grain hardness. Plant Physiol 151:2133–2144

    Article  PubMed  CAS  Google Scholar 

  • Reimer S, Pozniak CJ, Clarke FR, Clarke JM, Somers DJ, Knox RE, Singh AK (2008) Association mapping of yellow pigment in an elite collection of durum wheat cultivars and breeding lines. Genome 51:1016–1025

    Article  PubMed  CAS  Google Scholar 

  • Saintenac C, Faure S, Remay A, Choulet F, Ravel C, Paux E, Balfourier F, Feuillet C, Sourdille P (2011) Variation in crossover rates across a 3-Mb contig of bread wheat (Triticum aestivum) reveals the presence of a meiotic recombination hotspot. Chromosoma 120:185–198

    Article  PubMed  CAS  Google Scholar 

  • Wang JW, He XY, He ZH, Wang H, Xia XC (2009) Cloning and phylogenetic analysis of phytoene synthase 1 (Psy1) genes in common wheat and related species. Hereditas 146:208–256

    Article  PubMed  Google Scholar 

  • Ward J, Poutanen K, Gebruers K, Piironen V, Lampi AM, Nystrröm L, Anderson AM, Aman P, Boros D, Rakszegi M, Bedö Z, Shewry P (2008) The HEALTHGRAIN cereal diversity screen, concept, result and prospect. J Agric Food Chem 56:9699–9709

    Article  PubMed  CAS  Google Scholar 

  • Zhang W, Dubcovsky J (2008) Association between allelic variation at the phytoene synthase 1 gene and yellow pigment content in the wheat grain. Theor Appl Genet 116:635–645

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Rachel Carol and her team from Emendo Bioscience Ltd for English corrections. They are grateful to the staff of the Clermont-Ferrand INRA experimental unit led by B. Debote for field trials. Only sequences of novel alleles were deposited in Genbank. When sequences were 100 % identical for several lines, only one was deposited.

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Authors and Affiliations

  1. INRA, Blaise Pascal University, UMR1095 Genetics, Diversity and Ecophysiology of Cereals, 5 chemin de Beaulieu, 63100, Clermont-Ferrand, France

    Catherine Ravel, Mireille Dardevet, Jacques Bordes, Jean Louis Joseph, Marie Reine Perretant, Florence Exbrayat, Charles Poncet, François Balfourier & Gilles Charmet

  2. INRA, UMR 1019 UNH, 63122, Saint Genès-Champanelle, France

    Fanny Leenhardt

  3. Ulice, ZAC les portes de Riom, BP173, 63200, Riom, France

    Elisabeth Chanliaud

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  1. Catherine Ravel
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  2. Mireille Dardevet
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  3. Fanny Leenhardt
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  4. Jacques Bordes
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  5. Jean Louis Joseph
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  6. Marie Reine Perretant
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  7. Florence Exbrayat
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  8. Charles Poncet
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  9. François Balfourier
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  10. Elisabeth Chanliaud
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  11. Gilles Charmet
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Correspondence to Catherine Ravel.

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Ravel, C., Dardevet, M., Leenhardt, F. et al. Improving the yellow pigment content of bread wheat flour by selecting the three homoeologous copies of Psy1 . Mol Breeding 31, 87–99 (2013). https://doi.org/10.1007/s11032-012-9772-1

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