Abstract
Liquid biostimulants made from macroalgae have traditionally been based on the processing of brown seaweeds collected from the wild. In this study, liquid extracts were produced from the freshwater green macroalga Oedogonium intermedium, cultivated in a land-based system. The first part of the study was aimed at selecting extraction conditions to maximize the liquid yield and the recovery of soluble solids from Oedogonium biomass, which resulted in the selection of a biomass-to-solvent ratio of 5% (w/v), an extraction time of 4 h, and a temperature of 70 °C. These extraction conditions were used in the second part of the study to assess the effect of extraction solvent, across the whole pH range, on root growth of tomato and mung bean seedlings. Root growth of tomato seedlings was highest for the acid extract prepared with 0.05 M HCl, and the alkali extract prepared with 0.01 M KOH. Therefore, these two extracts were further tested in a mung bean assay to determine their auxin-like activity, and were also analyzed to determine their elemental and hormone profiles. The selected acid and alkali extracts, at concentrations of 10% and 2–10% (v/v), respectively, had an auxin-like activity equivalent to 5 × 10−5 M indole-3-butyric acid, with the alkali extract having the highest concentration of auxins (120 ng IAA g−1 dry weight). This study highlights the potential of O. intermedium for the production of liquid extracts with biostimulant activity.
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References
Anderson M, Gorley RN, Clarke RK (2008) Permanova+ for Primer: guide to software and statistical methods. Primer-E, Plymouth, p 214
APHA (2005) Standard methods for the examination of water and waste water, 21st edn. American Public Health Association, Washington, DC
Arioli T, Mattner SW, Winberg PC (2015) Applications of seaweed extracts in Australian agriculture: past, present and future. J Appl Phycol 27:2007–2015
Arthur GD, Aremu AO, Moyo M, Stirk WA, van Staden J (2013) Growth-promoting effects of a seaweed concentrate at various pH and water hardness conditions. S Afr J Sci 109:1–6
Augier H (1978) Les hormones des algues. Etat actuel des connaissances. VII-Applications, conclusion, bibliographie. Bot Mar 21:175–197
Briceño-Dominguez D, Hernández-Carmona G, Moyo M, Stirk WA, van Staden J (2014) Plant growth promoting activity of seaweed liquid extracts produced from Macrocystis pyrifera under different pH and temperature conditions. J Appl Phycol 26:2203–2210
Buschmann AH, Campus C, Infante J, Neori A, Israel A, Hernández-González MC, Pereda SV, Gomez-Pinchetti JL, Golberg A, Tadmor-Shalev N, Critchley AT (2017) Seaweed production: overview of the global state of exploitation, farming and emerging research activity. Eur J Phycol 52:391–406
Calvo P, Nelson L, Kloepper JW (2014) Agricultural uses of plant biostimulants. Plant Soil 383:3–41
Chauvaux N, Van Dongen W, Esmans EL, Van Onckelen HA (1997) Quantitative analysis of 1-aminocyclopropane-1-carboxylic acid by liquid chromatography coupled to electrospray tandem mass spectrometry. J Chromatogr A 775:143–150
Clarke KR, Gorley RN (2006) Primer v6: user manual/tutorial. Primer-E, Plymouth, p 190
Cole AJ, Neveux N, Whelan A, Morton J, Vis M, de Nys R, Paul NA (2016a) Adding value to the treatment of municipal wastewater through the intensive production of freshwater macroalgae. Algal Res 20:100–109
Cole AJ, Dinburg Y, Haynes BS, Herskowitz M, Jazrawi C, Landau M, Liang X, Magnusson M, Maschmeyer T, Masters AF, Meiri N, Neveux N, de Nys R, Paul NA, Rabaev M, Vidruk-Nehemya R, Yuen AKL (2016b) From macroalgae to liquid fuel via waste-water remediation, hydrothermal upgrading, carbon dioxide hydrogenation and hydrotreating. Energy Environ Sci 9:1828–1840
Cole AJ, Paul NA, de Nys R, Roberts DA (2017) Good for sewage treatment and good for agriculture: algal based compost and biochar. J Environ Manag 200:105–113
Craigie JS (2011) Seaweed extract stimuli in plant science and agriculture. J Appl Phycol 23:371–393
du Jardin P (2015) Plant biostimulants: definition, concept, main categories and regulation. Sci Hortic 196:3–14
EBIC (2017) Economic overview of the biostimulant sector in Europe. European Biostimulants Industry Council. http://www.biostimulants.eu/tag/biostimulants/
Entwisle TJ, Skinner S, Lewis SH, Foard HJ (2007) Algae of Australia: Batrachospermales, Thoreales. Oedogoniales and Zygnemaceae. CSIRO Publishing/Australian Biological Resources Study, Collingwood, Australia, p 200
Hernández-Herrera RM, Santacruz-Ruvalcaba F, Ruiz-López MA, Norrie J, Hernández-Carmona G (2014) Effect of liquid seaweed extracts on growth of tomato seedlings (Solanum lycopersicum L.). J Appl Phycol 26:619–628
Jarvis BC, Ali AHN, Shaheed AI (1983) Auxin and boron in relation to the rooting response and ageing of mung bean cuttings. New Phytol 95:509–518
Judd LA, Jackson BE, Fonteno WC (2015) Advancements in root growth measurement technologies and observation capabilities for container-grown plants. Plants 4:369–392
Khan W, Rayirath UP, Subramanian S, Jithesh MN, Rayorath P, Hodges DM, Critchley AT, Craigie JS, Norrie J, Prithiviraj B (2009) Seaweed extracts as biostimulants of plant growth and development. J Plant Growth Regul 28:386–399
Lawton RJ, de Nys R, Skinner S, Paul NA (2014) Isolation and identification of Oedogonium species and strains for biomass applications. PLoS One 9:e90223
Lawton RJ, Cole AJ, Roberts DA, Paul NA, de Nys R (2017) The industrial ecology of freshwater macroalgae for biomass applications. Algal Res 24:486–491
Lulsdorf MM, Yuan HY, Slater SMH, Vandenberg A, Han X, Zaharia LI, Abrams SR (2013) Endogenous hormone profiles during early seed development of C. arietinum and C. anatolicum. J Plant Growth Regul 71:191–198
Michalak I, Chojnacka K, Saeid A (2017) Plant growth biostimulants, dietary feed supplements and cosmetics formulated with supercritical CO2 algal extracts. Molecules 22:66
Middleton W, Jarvis BC, Booth A (1978) The boron requirement for root development in stem cuttings of Phaseolus Aureus Roxb. New Phytol 81:287–297
Neveux N, Yuen AKL, Jazrawi C, Magnusson M, Haynes BS, Masters AF, Montoya A, Paul NA, Maschmeyer T, de Nys R (2014) Biocrude yield and productivity from the hydrothermal liquefaction of marine and freshwater macroalgae. Bioresour Technol 155:334–341
Neveux N, Magnusson M, Maschmeyer T, de Nys R, Paul NA (2015) Comparing the potential production and value of high-energy liquid fuels and protein from marine and freshwater macroalgae. GCB Bioenergy 7:673–689
Neveux N, Magnusson M, Mata L, Whelan A, de Nys R, Paul NA (2016) The treatment of municipal wastewater by the macroalga Oedogonium sp. and its potential for the production of biocrude. Algal Res 13:284–292
Nissen S, Sutter E (1990) Stability of IAA and IBA in nutrient medium to several tissue culture procedures. HortScience 25:800–802
Reinhardt DH, Rost TL (1995) Primary and lateral root development of dark- and light-grown cotton seedlings under salinity stress. Bot Acta 108:457–465
Robert HS, Friml J (2009) Auxin and other signals on the move in plants. Nat Chem Biol 5:325–332
Roberts DA, Paul NA, Cole AJ, de Nys R (2015) From waste water treatment to land management: conversion of aquatic biomass to biochar for soil amelioration and the fortification of crops with essential trace elements. J Environ Manag 157:60–68
Sharma HSS, Fleming C, Selby C, Rao JR, Martin T (2014) Plant biostimulants: a review on the processing of macroalgae and use of extracts for crop management to reduce abiotic and biotic stresses. J Appl Phycol 26:465–490
Sharma HSS, Selby C, Carmichael E, McRoberts C, Rao JR, Ambrosino P, Chiurazzi M, Pucci M, Martin T (2016) Physicochemical analyses of plant biostimulant formulations and characterisation of commercial products by instrumental techniques. Chem Biol Technol Agric 3:13
Stirk WA, Ördög V, van Staden J, Jäger K (2002) Cytokinin- and auxin-like activity in Cyanophyta and microalgae. J Appl Phycol 14:215–221
Stirk WA, Arthur GD, Lourens AF, Novák O, Strnad M, van Staden J (2004) Changes in cytokinin and auxin concentrations in seaweed concentrates when stored at an elevated temperature. J Appl Phycol 16:31–39
Stirk WA, van Staden J (2006) Seaweed products as biostimulants in agriculture, world seaweed resources [DVD-ROM]: ETI Information Services Lts, Univ. Amsterdam, pp 80–84
Stirk WA, Bálint P, Tarkowská D, Novák O, Strnad M, Ördög V, van Staden J (2013) Hormone profiles in microalgae: gibberellins and brassinosteroids. Plant Physiol Biochem 70:348–353
Trejo-Téllez LI, Gómez-Merino FC (2012) Nutrient solutions for hydroponic systems, hydroponics-a standard methodology for plant biological researches. In Tech. http://www.intechopen.com/books/hydroponics-a-standardmethodology-forplant-biological-researches/nutrient-solutions-for-hydroponic-systems.
Walter A, Liebisch F, Hund A (2015) Plant phenotyping: from bean weighing to image analysis. Plant Methods 11:14
Wang C, Liu Y, Li SS, Han GZ (2014) Origin of plant auxin biosynthesis in charophyte algae. Trends Plant Sci 19:741–746
Yakhin OI, Lubyanov AA, Yakhin IA, Brown PH (2017) Biostimulants in plant science: a global perspective. Front Plant Sci 7:2049
Yamakawa T, Kurahashi O, Ishida K, Kato S, Kodama T, Minoda Y (1979) Stability of indole-3-acetic acid to autoclaving, aeration and light illumination. Agric Biol Chem 43:879–880
Acknowledgements
This research is part of the Pacific Bio Research and Development program for the integrated production of macroalgae. The authors would like to thank Anna Purcell, Shanae Read, and Dr. Alex Angell for assisting with the experimental design and data analysis.
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Neveux, N., Nugroho, A.A., Roberts, D.A. et al. Selecting extraction conditions for the production of liquid biostimulants from the freshwater macroalga Oedogonium intermedium. J Appl Phycol 32, 539–551 (2020). https://doi.org/10.1007/s10811-019-01925-3
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DOI: https://doi.org/10.1007/s10811-019-01925-3