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Using a wheat-rye amphihaploid population to map a rye gene responsible for dwarfness

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Abstract

Gene identification in cross-pollinating plants such as rye can be arduous and time consuming because of the difficulties involved with genetic population construction. Here, we provide an alternative approach for the construction of mapping populations to rapidly map genes in cross-pollinated cereal rye. The aim of the present experiments was to genetically analyze the dwarf stature expressed by a germplasm accession of rye. The dwarf phenotype was reversible when the seedlings were exposed to gibberellic acid; the reductions in plant height occurred via reductions in cell size. A mapping population was constructed by generating a set of wheat-rye amphihaploids bred from a single rye plant heterozygous for the dwarfing gene(s). The dwarfness phenotype was expressed in the amphihaploid background, and segregation in the mapping population was consistent with the presence of a single gene. Using rye SSR markers, the gene responsible was located on chromosome arm 1RL, which is also the location of the known rye dwarfing gene Ddw3. This gene is valuable for dwarf breeding of wheat as well as rye.

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Acknowledgements

We are grateful to Robert Koebner (smartenglish2008@gmail.com) for his help in improving the manuscript. We would also like to thank Dr. Bernd Hackauf and Peter Wehling, Federal Research Centre for Cultivated Plants Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut (JKI), Germany, for kindly providing the sequence information for the rye SSR markers. This research was financially supported by the Chinese National Natural Science Foundation (Grant Number 31771795) and the Sichuan Province Educational Commission’s Applied Basic Research Program (Grant Number 2017JY0142).

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, People’s Republic of China

    Sen Yang, Hongjing Zhu, Jianjun Yu, YuYang Zhong, LaiBin Zhao, YunFeng Jiang, Ming Hao, Lianquan Zhang, Shunzong Ning, XueJiao Chen, Dengcai Liu & Zhongwei Yuan

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  1. Sen Yang
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  2. Hongjing Zhu
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  3. Jianjun Yu
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  12. Zhongwei Yuan
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Contributions

SY, ZY and DL designed the study. SY, HZ, JY, YZ, LZ and XC created the mapping and verification population. SY, HZ and JY carried out molecular genotyping. SY, YJ and MH analyzed the data. SY, ZY and DL drafted the manuscript; LZ and SN participated in planning the study. All authors read and approved the final manuscript.

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Correspondence to Dengcai Liu or Zhongwei Yuan.

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The authors declare that all experiments complied with the current laws of China.

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Yang, S., Zhu, H., Yu, J. et al. Using a wheat-rye amphihaploid population to map a rye gene responsible for dwarfness. Euphytica 214, 166 (2018). https://doi.org/10.1007/s10681-018-2247-7

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