Abstract
Calcium-dependent protein kinases (CDPKs) can decode upstream Ca2+ flux changes and then phosphorylate downstream signaling molecules while transducing Ca2+ signaling, thereby engaging in plant growth, development, and biotic and abiotic stress responses. Although the CDPK gene family has been identified and analyzed in various plants, knowledge concerning CDPKs in woodland strawberry (Fragaria vesca) and their expression profiles in response to biotic stresses remain very limited. In this study, we identified 19 CDPK genes in F. vesca. Phylogenetic analysis separated the FvCDPKs into 4 subgroups. Gene structure and protein motif analysis for these FvCDPKs illustrated the different evolutionary relationships within subgroups. Collinearity analysis revealed 18 syntenic pairs between AtCDPKs and FvCDPKs and 5 syntenic pairs between OsCDPKs and FvCDPKs. Biotic stress-related cis-acting elements were detected in the promoter regions of FvCDPKs. Expression profiling of FvCDPKs was performed in different tissues under different biotic stresses, including Phytophthora cactorum (PC), Botrytis cinerea (BC) and strawberry vein banding virus (SVBV) exposure. The expression pattern of FvCDPKs during pathogen infection demonstrated their distinctive functional responses to biotic stresses. In summary, this study provides new insight into FvCDPKs and their response under biotic stresses.
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This research was funded by the Natural Science Foundation of Fujian Province (2019J01809, 2021J05240, 2022J011162), the Fujian Provincial Science and Technology Project (2019N0022, 2021N5014), the Education and Research Project of Young and Middle-Aged Teachers of Fujian Province (JAT170501, JT180468, JAT200524), the Research Project of Putian Science and Technology Bureau (2021ZP08, 2021ZP10, 2020NP001), Research Foundation of Putian University (2020001), the Research and Innovation Special Foundation of Putian University (2016CX001), and the Scientific Research Project of Putian University (2018058, 2018064). This manuscript was edited by American Journal Experts (AJE) for proper English language use.
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Xiong, Y., Lin, D., Ma, S. et al. Genome-wide identification of the calcium-dependent protein kinase gene family in Fragaria vesca and expression analysis under different biotic stresses. Eur J Plant Pathol 164, 283–298 (2022). https://doi.org/10.1007/s10658-022-02560-4
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DOI: https://doi.org/10.1007/s10658-022-02560-4