Skip to main content
SpringerLink
Log in

Characterization of HbEREBP1, a wound-responsive transcription factor gene in laticifers of Hevea brasiliensis Muell. Arg.

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

AP2/ERF transcription factors play an important role in regulation of the cross-talk between ethylene and jasmonate signaling pathways mediating defense responses of plants to biotic and abiotic stresses. In this study, an AP2/ERF transcription factor gene was isolated and characterized from laticifers of rubber tree by using RACE and real time PCR. The full length cDNA, referred to as HbEREBP1, was 1,095 bp in length and contained a 732 bp open reading frame encoding a putative protein of 243 amino acid residues. The molecular mass of the putative protein is 26.4 kDa with a pI of 9.46. The deduced amino acid sequence had a specific domain of AP2 superfamily and an ethylene-responsive element binding factor-associated amphiphilic repression motif, sharing 42.4, 39.1, and 38.0% identity with that of AtERF11, AtERF4, and AtERF8 in Arabidopsis, respectively. HbEREBP1 expression was down-regulated by tapping and mechanical wounding in the laticifers of adult trees. It was also down-regulated at early stage while up-regulated at late stage upon treatment with exogenous ethephon or methyl jasmonate, which was reverse to the case of defense genes in laticifers of epicormic shoots of rubber tree. Our results suggest that HbEREBP1 may be a negative regulator of defense genes in laticifers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Gutterson N, Reuber TL (2004) Regulation of disease resistance pathways by AP2/ERF transcription factors. Curr Opin Plant Biol 7(4):465–471. doi:10.1016/j.pbi.2004.04.007

    Article  PubMed  CAS  Google Scholar 

  2. Ohme-Takagi M, Shinshi H (1995) Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. Plant Cell 7(2):173–182. doi:10.1105/tpc.7.2.173

    Article  PubMed  CAS  Google Scholar 

  3. Nakano T, Suzuki K, Fujimura T, Shinshi H (2006) Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol 140(2):411–432. doi:10.1104/pp.105.073783

    Article  PubMed  CAS  Google Scholar 

  4. Hanna-Rose W, Hansen U (1996) Active repression mechanisms of eukaryotic transcription repressors. Trends Genet 12(6):229–234. doi:10.1016/0168-9525(96)10022-6

    Article  PubMed  CAS  Google Scholar 

  5. Fujimoto SY, Ohta M, Usui A, Shinshi H, Ohme-Takagi M (2000) Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. Plant Cell 12(3):393–404

    Article  PubMed  CAS  Google Scholar 

  6. Yang Z, Tian L, Latoszek-Green M, Brown D, Wu K (2005) Arabidopsis ERF4 is a transcriptional repressor capable of modulating ethylene and abscisic acid responses. Plant Mol Biol 58(4):585–596. doi:10.1007/s11103-005-7294-5

    Article  PubMed  CAS  Google Scholar 

  7. Kagale S, Rozwadowski K (2011) EAR motif-mediated transcriptional repression in plants: an underlying mechanism for epigenetic regulation of gene expression. Epigenetics 6(2):141–146

    Google Scholar 

  8. de Fay E, Hebant C, Jacob JL (1989) Cytology and cytochemistry of the laticiferous system. Physiology of rubber tree latex: the laticiferous cell and latex-A model of cytoplasm. CRC Press Inc., Boca Raton

    Google Scholar 

  9. Hao BZ, Wu JL, Yun CY (1984) Acceleration of laticifer differentiation in Hevea brasiliensis by latex drainage. Chin J Trop Crops 5(2):19–23

    Google Scholar 

  10. Coupe M, Chrestin H (1989) Physico-Chemical and biochemical mechanisms of hormonal (ethylene) stimulation. In: D’Auzac J, Jacob JL, Chrestin H (eds) Physiology of rubber tree latex: the laticiferous cell and latex-A model of cytoplasm. CRC Press, Inc., Boca Raton, pp 295–320

    Google Scholar 

  11. Gidrol X, Chrestin H, Mounoury G, D’Auzac J (1988) Early activation by ethylene of the tonoplast H-pumping ATPase in the latex from Hevea brasiliensis. Plant Physiol 86(3):899–903

    Article  PubMed  CAS  Google Scholar 

  12. Tang C, Huang D, Yang J, Liu S, Sakr S, Li H, Zhou Y, Qin Y (2010) The sucrose transporter HbSUT3 plays an active role in sucrose loading to laticifer and rubber productivity in exploited trees of Hevea brasiliensis (para rubber tree). Plant Cell Environ 33(10):1708–1720. doi:10.1111/j.1365-3040.2010.02175.x

    Article  PubMed  CAS  Google Scholar 

  13. Zhu J, Zhang Z (2009) Ethylene stimulation of latex production in Hevea brasiliensis. Plant Signal Behav 4(11):1072–1074

    Article  PubMed  CAS  Google Scholar 

  14. Browse J (2005) Jasmonate: an oxylipin signal with many roles in plants. Vitam Horm 72:431–456. doi:10.1016/S0083-6729(05)72012-4

    Article  PubMed  CAS  Google Scholar 

  15. Balbi V, Devoto A (2008) Jasmonate signalling network in Arabidopsis thaliana: crucial regulatory nodes and new physiological scenarios. New Phytol 177(2):301–318. doi:10.1111/j.1469-8137.2007.02292.x

    Article  PubMed  CAS  Google Scholar 

  16. Zhou M-L, Zhu X-M, Shao J-R, Wu Y-M, Tang Y-X (2010) Transcriptional response of the catharanthine biosynthesis pathway to methyl jasmonate/nitric oxide elicitation in Catharanthus roseus hairy root culture. Appl Microbiol Biotechnol 88(3):737–750. doi:10.1007/s00253-010-2822-x

    Article  PubMed  CAS  Google Scholar 

  17. Hao B-Z, Wu J-L (2000) Laticifer differentiation in Hevea brasiliensis: Induction by exogenous jasmonic acid and linolenic acid. Ann Bot 85:37–43

    Article  CAS  Google Scholar 

  18. Kombrink E, Schroder M, Hahlbrock K (1988) Several “pathogenesis-related” proteins in potato are 1,3-beta-glucanases and chitinases. Proc Natl Acad Sci USA 85(3):782–786

    Article  PubMed  CAS  Google Scholar 

  19. Ko JH, Chow KS, Han KH (2003) Transcriptome analysis reveals novel features of the molecular events occurring in the laticifers of Hevea brasiliensis (para rubber tree). Plant Mol Biol 53(4):479–492. doi:10.1023/B:PLAN.0000019119.66643.5d

    Article  PubMed  CAS  Google Scholar 

  20. Kush A, Goyvaerts E, Chye ML, Chua NH (1990) Laticifer-specific gene expression in Hevea brasiliensis (rubber tree). Proc Natl Acad Sci USA 87(5):1787–1790

    Article  PubMed  CAS  Google Scholar 

  21. Xiao X, Li H, Tang C (2009) A silver-staining cDNA-AFLP protocol suitable for transcript profiling in the latex of Hevea brasiliensis (para rubber tree). Mol Biotechnol 42(1):91–99. doi:10.1007/s12033-008-9139-3

    Article  PubMed  CAS  Google Scholar 

  22. Tang C, Qi J, Li H, Zhang C, Wang Y (2007) A convenient and efficient protocol for isolating high-quality RNA from latex of Hevea brasiliensis (para rubber tree). J Biochem Biophys Methods 70(5):749–754. doi:10.1016/j.jbbm.2007.04.002

    Article  PubMed  CAS  Google Scholar 

  23. Kitajima S, Koyama T, Ohme-Takagi M, Shinshi H, Sato F (2000) Characterization of gene expression of NsERFs, transcription factors of basic PR genes from Nicotiana sylvestris. Plant Cell Physiol 41(6):817–824

    PubMed  CAS  Google Scholar 

  24. Harfouche AL, Rugini E, Mencarelli F, Botondi R, Muleo R (2008) Salicylic acid induces H2O2 production and endochitinase gene expression but not ethylene biosynthesis in Castanea sativa in vitro model system. J Plant Physiol 165(7):734–744. doi:10.1016/j.jplph.2007.03.010

    Article  PubMed  CAS  Google Scholar 

  25. Okamuro JK, Caster B, Villarroel R, Van Montagu M, Jofuku KD (1997) The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proc Natl Acad Sci USA 94(13):7076–7081

    Article  PubMed  CAS  Google Scholar 

  26. Ohta M, Matsui K, Hiratsu K, Shinshi H, Ohme-Takagi M (2001) Repression domains of class II ERF transcriptional repressors share an essential motif for active repression. Plant Cell 13(8):1959–1968

    Article  PubMed  CAS  Google Scholar 

  27. Hao B-Z, Wu J-L, Meng C-X, Gao Z-Q, Tian H-Y (2004) Laticifer wound plugging in Hevea brasiliensis: The role of a protein-network with rubber particle aggregations in stopping latex flow and protecting wounded laticifers. J Rubber Res 7(4):281–299

    Google Scholar 

  28. Yang S-G, Yu J-H, Shi M-J, Chen Y-Y, Tian W-M (2008) Effects of tapping, mechanical wounding and expgenous jasmonates on the expression of genes encoding for defense proteins in the latex of Hevea brasiliensis. Chin J of Trop Crops 29(5):535–540

    Google Scholar 

  29. McGrath KC, Dombrecht B, Manners JM, Schenk PM, Edgar CI, Maclean DJ, Scheible WR, Udvardi MK, Kazan K (2005) Repressor- and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression. Plant Physiol 139(2):949–959. doi:10.1104/pp.105.068544

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the earmarked fund for China Agriculture Research System (CARS-34-GW1) and Chinese National Nonprofit Institute Research Grant of CATAS-RRI (XJSYWFZX2009-09), and equally contributed by the Rubber Research Institute, CATAS and Hainan University.

Author information

Authors and Affiliations

  1. Ministry of Agriculture Key Laboratory for Rubber Biology, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, 571737, China

    Yue-Yi Chen, Li-Feng Wang, Long-Jun Dai, Shu-Guang Yang & Wei-Min Tian

  2. College of Horticulture and Landscape Architecture, Hainan University, Danzhou, Hainan, 571737, China

    Yue-Yi Chen

Authors
  1. Yue-Yi Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Li-Feng Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Long-Jun Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shu-Guang Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wei-Min Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wei-Min Tian.

Electronic supplementary material

Below is the link to the electronic supplementary material.

11033_2011_1146_MOESM1_ESM.jpg

Fig. S1 Sequence alignment of the deduced HbEREBP1 protein and other ERF proteins. The HbEREBP1 (GenBank accession No.: HQ171930) was from Hevea brasiliensis while AtERF3 (GenBank accession No.: GI7531109), AtERF4 (GenBank accession No.: GI7531110), AtERF11 (GenBank accession No.:GI57012863) were from Arabidopsis, and NsERF3 (GenBank accession No.: GI57012875) was from Nicotiana sylvestris. The identical amino acids were shaded in dark and the well-conserved residues were shaded in gray. The conserved domain and motif was underlined. Supplementary material 1 (JPEG 352 kb)

11033_2011_1146_MOESM2_ESM.jpg

Fig. S2 Phylogenetic tree of HbEREBP1 from rubber tree and ERF-like members from Arabidopsis with indicated GenBank accession numbers. The gene names instead of GenBank accession numbers were given for the members of B1a subgroup. Supplementary material 2 (JPEG 56 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, YY., Wang, LF., Dai, LJ. et al. Characterization of HbEREBP1, a wound-responsive transcription factor gene in laticifers of Hevea brasiliensis Muell. Arg.. Mol Biol Rep 39, 3713–3719 (2012). https://doi.org/10.1007/s11033-011-1146-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-011-1146-y

Keywords

Search

Navigation