Abstract
Calcium-dependent protein kinases (CDPKs) play significant roles in response to environmental stresses in plants. However, the CDPK gene family in pear (Pyrus bretschneideri Rehd) has not been thoroughly explored. In this study, we performed a comprehensive analysis of CDPK family genes in pear, and 30 CDPK genes were identified, unevenly distributed across 13 chromosomes, and predicted to be mainly localized at the plasma membrane. Based on phylogenetic analysis and structural features, the PbCDPKs were classified into four distinct subfamilies. In addition, many putative cis-acting elements responsive to hormones and environmental stresses were identified in the promoter regions. According to RNA-seq data, the majority of PbCDPK genes showed differential expression in different pear tissues. After inoculation of plants with the fungus Venturia nashicola, four PbCDPK genes were differentially expressed in resistant and susceptible pear cultivars, suggesting that they might play an important role in resistance to the pear scab pathogen. These candidate genes will be subjected to disease resistance and functional analysis in future work. Our results thus provide a genome-wide characterization of CDPK family genes in pear and lay the foundation for further functional analysis.
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Data availability
All data used and analyzed during this research are included in this article and its additional files. The pear genome database used during the current study are available on the website (http://gigadb.org/search/new?keyword=Pyrus).
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Acknowledgements
The authors thank all contributors to this research for their help. This research was financially supported by the National Pear Industry Technology Research System of China (Grant No. CARS-29-05), and the National Key Research and Development Plan of China (Grant No. 2018YFD0201407), and the Local Funds for Scientific and Technological Development from Central Government (Grant No. YDZJSX2022A042).
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SL contributed by methodology, data curation, writing the manuscript. JL contributed by checking the experimental data. NL contributed to the manuscript editing. PZ contributed by methodology. LL contributed by conceptualization, supervision, manuscript editing. All authors have read and approved the final manuscript.
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Liu, S., Li, J., Li, N. et al. Genome-wide identification of calcium-dependent protein kinases (CDPKs) in pear (Pyrus bretschneideri Rehd) and characterization of their responses to Venturia nashicola infection. Hortic. Environ. Biotechnol. 63, 903–915 (2022). https://doi.org/10.1007/s13580-022-00444-4
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DOI: https://doi.org/10.1007/s13580-022-00444-4