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Immobilization of lactoperoxidase on graphene oxide nanosheets with improved activity and stability

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Abstract

Objectives

The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS).

Methods

Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using glutaraldehyde as cross-linker. Kinetic properties and stability of free and immobilized LPO were investigated.

Results

LPO was purified 59.13 fold with a specific activity of 5.78 U/mg protein. The successful immobilization of LPO on functionalized GO-NS was confirmed by using dynamic light scattering (DLS) and Fourier transform infrared spectroscopy (FT-IR). The overall results showed that the stability of the immobilized LPO was considerably improved compared to free LPO. Apparent Km and Vmax of LPO also indicated that the immobilized enzyme had greater affinity to the substrate than the native enzyme.

Conclusions

Graphene oxide nanosheets are effective means for immobilization of LPO.

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Acknowledgements

The present work was supported by the Grant no. 393740 from Isfahan University of Medical Sciences.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Bioinformatics Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Esfahān, Iran

    Seyed Ziyae Aldin Samsam Shariat, Fatemeh Borzouee & Mohammad Reza Mofid

  2. Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Esfahān, Iran

    Jaleh Varshosaz

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  1. Seyed Ziyae Aldin Samsam Shariat
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  2. Fatemeh Borzouee
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  3. Mohammad Reza Mofid
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  4. Jaleh Varshosaz
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Correspondence to Jaleh Varshosaz.

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Shariat, S.Z.A.S., Borzouee, F., Mofid, M.R. et al. Immobilization of lactoperoxidase on graphene oxide nanosheets with improved activity and stability. Biotechnol Lett 40, 1343–1353 (2018). https://doi.org/10.1007/s10529-018-2583-7

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  • DOI: https://doi.org/10.1007/s10529-018-2583-7

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